Isolation of Anti-Mannose Antibodies By Affinity Chromatography On Mannosyl-Sepharose

Abstract

Anti-a-Q-mannose antibodies have been isolated from the serum of rabbits immunized with the glycoconjugate of a-Q-mannose and bovine semm albumin joined by aminophenyI bridges. The antibodies were purified by affinity chromatography with adsorption on a mannosyl-Sepharose 4B column and elution with a-2-mannose or methyl a-D-mannoside. Such antibodies should be especially useful for studying the Gtection and progression of diseases caused by viruses, pathogenic microorganisms or transformed cells and could be useful as curative agents.

Generation of antibodies specific to d-mannitol, a unique haptenic allergen, using reductively aminated d-mannose–bovine serum albumin conjugate as the immunogen

“…These antibodies are generally specific to cyclic pyranose or furanose structures of sugars. Although antibodies to D-mannose (Pazur et al, 1992), mannotetraose (Zopf et al, 1978), and mannosyl trisaccharide (Vargas et al, 1992) have been described using p-aminophenyl glycosides of the respective saccharides covalently coupled to a carrier protein as synthetic antigens, an anti-carbohydrate antibody specific to D-mannitol, to our knowledge, has not been reported so far.…”