Isolation of anoxygenic photosynthetic bacteria from Songkhla Lake for use in a two-staged biohydrogen production process from palm oil mill effluent

Breakdown of food waste by anaerobic fermentation and non-oxygen producing photosynthesis using a photosynthetic bacterium

“…Sterile food waste was used in this study to ensure that any effects on NH 3 , NO 3 -, P, BOD, COD and gas production must have been due to the Rhodopseudomonas inoculum and not a result of the metabolism of any organisms already present.Raw food waste would contain many different and highly variable populations of microbes.A particular advantage of Rhodopseudomonads is that they are able to readily break down lipids. R. palustris can produce hydrogen gas from palm oil milling effluent which has a very high oil and fatty acid content (VFAs-Volatile Fatty Acids) (Suwansaard et al, 2009). There are very few organic compounds which Rhodopseudomonas cannot use as a carbon source (Larimer et al, 2004;KEGG, 2013).…”

“…Rhodopseudomonas strains are also capable of hydrogen gas production both anaerobically in the dark and photosynthetically using organic compounds as a hydrogen source (Hillmer and Gest, 1977;Mangels et al, 1986;Suwansaard et al, 2009;Panwichian, et al, 2010;Suwansaard et al, 2010) R. palustris can produce hydrogen gas from palm oil milling effluent which has a very high oil and fatty acid content (VFAs-Volatile Fatty Acids) (Suwansaard et al, 2009).…”

“…The isolate NW16 removed a greater percentage of the heavy metals than the isolate KMS24. Furthermore, a strain of Rhodopseudomonas palustris, TN1 isolated from Songkhla Lake in Southern Thailand was found to be an effective producer of hydrogen gas (Suwansaard et al, 2009;Suwansaard et al, 2010) from palm oil mill effluent. Rhodopseudomonas palustris, TN1 was also shown to be able to grow on VFAs (Volatile Fatty Acids) and will also grow on added acetate, butyrate, and propionate as carbon sources.…”

“…The BOD and COD of the food is comparable to industrial effluent from olive mill waste which has been successfully broken down using Rhodopseudomonas (Pintucci et al, 2013). Rhodopseudomonas has also been used successfully to break down palm oil mill waste (Suwansaard et al, 2009). R. palustris is also very efficient in breaking down fatty acids which would arise from the hydrolysis of cooking oils (Choorit et al, 2011).…”

“…Figs 4.26 and 4.27 show daily production of H 2 and cumulative production of H 2 over the 7 days of the incubation experiment. Nitrogen fixation can increase the H 2 production yield from nitrogenase but H 2 production can occur without N 2 fixation but it is important to know that H 2 production is highly variable both between strains and under different growth conditions (Suwansaard et al, 2009). Rhodopseudomonas palustris has the peculiarity of encoding three different nitrogenases in its genome (Larimer et al, 2004;Oda et al, 2005).…”