Isolation of Angola-like Marburg virus from Egyptian rousette bats from West Africa

Amman, Brian R.; Bird, Brian H.; Bakarr, Ibrahim A.; Bangura, James; Schuh, Amy J.; Johnny, Jonathan; Sealy, Tara K.; Conteh, Immah; Koroma, A H; Foday, I. K.; Amara, Emmanuel; Bangura, Abdulai A.; Gbakima, Aiah A; Tremeau‐Bravard, Alexandre; Belaganahalli, Manjunatha N.; Dhanota, Jasjeet K.; Chow, Andrew; Ontiveros, Victoria; Gibson, Alexandra A.; Turay, Joseph A; Patel, Ketan; Graziano, James; Bangura, Camilla; Kamanda, Emmanuel S.; Osborne, Augustus; Saidu, Emmanuel; Musa, Jonathan; Bangura, Doris; Williams, Samuel Maxwell Tom; Wadsworth, Richard A.; Turay, Mohamed; Edwin, Lavalie; Mereweather-Thompson, Vanessa; Kargbo, Dickson; Bairoh, Fatmata V.; Kanu, Marilyn C; Robert, Willie; Lungai, Victor; Wadoum, Raoul Emeric Guetiya; Coomber, Moinya; Kanu, Osman T.; Jambai, Amara; Kamara, Sorie Mohamed; Taboy, Céline H.; Singh, Tushar; Mazet, Jonna A. K.; Nichol, Stuart T.; Goldstein, Tracey; Towner, Jonathan S.; Lebbie, Aiah

doi:10.1038/s41467-020-14327-8

Cited by 85 publications

(92 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ebola virus (EBOV) and Marburg virus (MARV), belonging to the genera Ebolavirus and Marburgvirus , respectively, of the family Filoviridae , cause severe hemorrhagic fever in humans and nonhuman primates [ 1 , 2 ]. While the ecology of EBOV and MARV is largely unknown, some species of fruit bats are suspected to be natural reservoirs of these viruses [ 3 , 4 , 5 , 6 , 7 ]. In 2002, a novel filovirus, Lloviu virus (LLOV), phylogenetically distinct from the viruses in the genera Ebolavirus and Marburgvirus , was discovered in carcasses of insectivorous bats (Schreiber’s bent-winged bat: Miniopterus schreibersii ) in Spain [ 8 ] and this virus was designated a new filovirus member belonging to the genus Cuevavirus [ 2 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus

et al. 2020

View full text Add to dashboard Cite

Lloviu virus (LLOV), a bat-derived filovirus that is phylogenetically distinct from human pathogenic filoviruses such as Ebola virus (EBOV) and Marburg virus (MARV), was discovered in Europe. However, since infectious LLOV has never been isolated, the biological properties of this virus remain poorly understood. We found that vesicular stomatitis virus (VSV) pseudotyped with the glycoprotein (GP) of LLOV (VSV–LLOV) showed higher infectivity in one bat (Miniopterus sp.)-derived cell line than in the other bat-derived cell lines tested, which was distinct from the tropism of VSV pseudotyped with EBOV (VSV–EBOV) and MARV GPs. We then focused on the interaction between GP and Niemann–Pick C1 (NPC1) protein, one of the cellular receptors of filoviruses. We introduced the Miniopterus bat and human NPC1 genes into NPC1-knockout Vero E6 cells and their susceptibilities to the viruses were compared. The cell line expressing the bat NPC1 showed higher susceptibility to VSV–LLOV than that expressing human NPC1, whereas the opposite preference was seen for VSV–EBOV. Using a site-directed mutagenesis approach, amino acid residues involved in the differential tropism were identified in the NPC1 and GP molecules. Our results suggest that the interaction between GP and NPC1 is an important factor in the tropism of LLOV to a particular bat species.

show abstract

Section: Introductionmentioning

confidence: 99%

Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Recently there is an emerging interest in bats since they recognized to be hosts for large swath of pathogens that affect humans and livestock [ 3 ]. In fact, bats are considered the natural reservoir of a large variety of zoonotic viruses including henipaviruses [ 4 ], filoviruses [ 5 ], some lyssaviruses [ 6 ], SARS-CoV [ 7 ], Middle East Respiratory Syndrome [ 8 ] and SARS-CoV-2 [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…The Egyptian fruit bat is the only megachrioptera amongthese species and the most common bat found in close proximity to humans in urban areas, national parks and nature reserves [ 10 ]. As true for other bats species, Egyptian fruit bats can harbor many different viruses without showing clinical signs and are the natural reservoir for the Marburg [ 5 ] and Sosuga viruses [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Israeli Rousettus aegyptiacus Pox Virus (IsrRAPXV) Infection in Juvenile Egyptian Fruit Bat (Rousettus aegyptiacus): Clinical Findings and Molecular Detection

David¹,

Davidson²,

Karniely³

et al. 2021

Viruses

View full text Add to dashboard Cite

During 2019, five carcasses of juvenile Egyptian fruit bats (Rousettus aegyptiacus) were submitted to the Kimron Veterinary Institute. These bats exhibited typical poxvirus like lesion plaques of different sizes on the skin, abdomen and the ventral side of the wings. Clinical and histopathological findings suggested a poxvirus infection. Infectious virus was isolated from skin swabs, skin tissue and tongue of the dead bats and was further confirmed to be a Poxvirus by molecular diagnosis using PCR with pan-chordopoxviruses primers. All the dead bats were found positive for two Poxvirus genes encoding a metalloproteinase and DNA dependent DNA polymerase. In this study, a novel real time quantitative PCR (qPCR) assay was established to further confirmed the presence of specific poxvirus viral DNA in all pathologically tested tissues. Moreover, according to sequence analysis, the virus was found to be highly similar to the recently discovered Israeli Rousettus aegyptiacus Pox Virus (IsrRAPXV).

show abstract

“…MARV has been isolated from the Egyptian rousette bat (ERB, Rousettus aegyptiacus) [5][6][7] , and ecological and experimental studies have demonstrated that ERB are a reservoir for the virus 6,8 . Experimental infections of ERBs with MARV have consistently demonstrated that despite viral replication in multiple tissues, animals develop a mostly subclinical disease, which is characterized by mild pathology involving transient elevation of alanine aminotransferase, elevated lymphocyte and monocyte counts, and some evidence of minimal inflammatory infiltration in the liver 9,10 .…”

Section: Introductionmentioning

confidence: 99%

Marburg and Ebola virus infections elicit a complex, muted inflammatory state in bats

Jayaprakash¹,

Ronk

Prasad

et al. 2020

Preprint

View full text Add to dashboard Cite

The filoviruses Ebola (EBOV) and Marburg (MARV) cause severe disease in humans. In contrast, the Egyptian rousette bat (Rousettus aegyptiacus), a natural reservoir of MARV, exhibits a subclinical phenotype with limited MARV replication and nearly undetectable EBOV replication. Rousettus cell lines support replication of filoviruses, however. To understand the bat-filovirus interaction, transcriptomes of tissues from EBOV-and MARV-infected R. aegyptiacus bats were analyzed. While viral transcripts were only detected in liver, a systemic response was observed involving other tissues as well. By focusing on evolutionarily divergent (from human homologues) protein-coding genes, we identified novel transcriptional pathways that suggest infected bats exhibit impaired coagulation, vasodilation, aberrant iron regulation, and impaired complement system leading to muted antibody responses. Furthermore, a robust T-cell response and an antiinflammatory state driven by M2 macrophages were identified. These processes likely control infection and limit pathology. All data can be freely explored and downloaded through our tools (http://katahdin.girihlet.com/shiny/bat/). bat-filovirus-jayaprakashWe experimentally infected Egyptian rousettes with EBOV and MARV and analyzed mRNA transcripts from multiple tissue types: liver, kidney, spleen, peripheral blood mononuclear cells (PBMCs), and testes. For that purpose, we compiled a comprehensive list of bat transcripts, based on our sequence data together with existing genome annotations 28 , and, where possible, identified genes with human or mouse homologues.The majority of the differentially-expressed genes upon filoviral infection were common to bats and humans 29 . These genes include components of a number of pathways involved in the innate, inflammatory, acute phase, and adaptive immune responses, as well as in the activation of the complement/coagulation pathway. Despite this broad similarity of the responses, filoviral infections in vivo result in very different outcomes in bats compared to humans. bat-filovirus-jayaprakash 2 bat-filovirus-jayaprakash 4

show abstract

Isolation of Angola-like Marburg virus from Egyptian rousette bats from West Africa

Cited by 85 publications

References 47 publications

Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus

Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus

Israeli Rousettus aegyptiacus Pox Virus (IsrRAPXV) Infection in Juvenile Egyptian Fruit Bat (Rousettus aegyptiacus): Clinical Findings and Molecular Detection

Marburg and Ebola virus infections elicit a complex, muted inflammatory state in bats

Contact Info

Product

Resources

About