Isolation of Andrographis paniculata Leaf Protein with Antifungal Property

Ghosh, Modhumita; Thangamani, D.; Thapliyal, Manisha; Yasodha, R.; Gurumurthi, K.

doi:10.1556/aphyt.39.2004.4.5

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…Initially, standard extraction procedures were followed that are normally used for the disruption of cells. 40 Thus, repeated detergent-based lysis and corresponding sonication methods were applied to six repeat extraction samples of SEC. 41 Attempts to detect the presence of proteins in the extracts by SDS-PAGE and the traditional Lowry method proved unsuccessful with all of the samples.…”

Section: Resultsmentioning

confidence: 99%

“…The pellet was discarded and the supernatant was desalted using a Sephadex G-50 column and the eluent (acetonitrile-water) was evaporated: the residue was dissolved in water, lyophilized and used for protein identication. 40 SONICATION LYSIS PROTEIN EXTRACTION OF SPOROPOLLENIN. The protocol followed was in accordance with that report by Bruggeman and Westerhoff 41 SEC (100 mg) were pulverized in liquid nitrogen using a mortar and pestle, sonicated in trichloroacetic acid : acetone (1 : 9 w/v) solution in a sonic water bath.…”

Section: Taste Trialmentioning

confidence: 99%

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Protein free microcapsules obtained from plant spores as a model for drug delivery: ibuprofen encapsulation, release and taste masking

et al. 2013

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Sporopollenin exine capsules (SEC) extracted from Lycopodium clavatum spores were shown to encapsulate ibuprofen as a drug model, with 97 AE 1% efficiency as measured by recovery of the loaded drug and absence of the drug on the SEC surface by scanning electron microscopy (SEM). The encapsulated ibuprofen was shown to be unchanged from its bulk crystalline form by solid state NMR, FTIR and XRD. Essential for drug delivery applications, SEC were shown to be non-toxic to human endothelial cells and free of allergenic protein epitopes by MALDI-TOF-MS and ESI-QqToF-MS. Potential application for targeted release into the intestinal region of the gastrointestinal tract (GIT) was demonstrated by 88 AE 1% of the drug being retained in simulated gastric fluid (SGF) after 45 minutes and 85 AE 2% being released after 5 min in buffer (PBS; pH 7.4). The SEC were shown to provide significant taste masking of encapsulated ibuprofen in a double blind trial with 10 human volunteers.

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Section: Resultsmentioning

confidence: 99%

Section: Taste Trialmentioning

confidence: 99%

Protein free microcapsules obtained from plant spores as a model for drug delivery: ibuprofen encapsulation, release and taste masking

et al. 2013

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“…Atta-ur-Rahman and Choudhary (1995) stated that diterpenoid alkaloids are commonly found to have anti-microbial properties. Similarly, Ghosh et al, (2004) reported that the crude protein extract from the leaves of Andrographis paniculata was found to inhibit the spore germination of two major pathogens Aspergillus flavus and Macrophomina phaseolina.…”

Section: Anti-fungal Activity Of Different Solvent Extractsmentioning

confidence: 95%