Isolation of an Ozone-Sensitive and Jasmonate-Semi-Insensitive Arabidopsis Mutant (oji1)

Kanna, Machi; Tamaoki, Masanori; Kubo, Akihiro; Nakajima, Nobuyoshi; Rakwal, Randeep; Agrawal, Ganesh Kumar; Tamogami, Shigeru; Ioki, Motohide; Ogawa, Daisuke; Saji, Hikaru; Aono, Mitsuko

doi:10.1093/pcp/pcg157

Cited by 54 publications

(57 citation statements)

References 44 publications

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“…Interestingly, JA signaling is also linked to ROS. For example, JA induces the accumulation of antioxidant pathways, and in a JA-deficient Arabidopsis mutant (opr3) the induction of these pathways upon ozone exposure is abolished (Sasaki-Sekimoto et al, 2005), and oji1, a JA-insensitive mutant, is hypersensitive to O 3 treatments (Kanna et al, 2003). On the other hand, JA generates H 2 O 2 , an agent we found to activate MPK7, as a secondary signal for the activation of defense genes (Orozco-Cardenas and Ryan, 1999;Orozco-Cardenas et al, 2001).…”

Section: Discussionmentioning

confidence: 77%

The Arabidopsis Mitogen-Activated Protein Kinase Kinase MKK3 Is Upstream of Group C Mitogen-Activated Protein Kinases and Participates in Pathogen Signaling

et al. 2007

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Although the Arabidopsis thaliana genome contains genes encoding 20 mitogen-activated protein kinases (MAPKs) and 10 MAPK kinases (MAPKKs), most of them are still functionally uncharacterized. In this work, we analyzed the function of the group B MAPK kinase, MKK3. Transgenic ProMKK3:GUS lines showed basal expression in vascular tissues that was strongly induced by Pseudomonas syringae pv tomato strain DC3000 (Pst DC3000) infection but not by abiotic stresses. The growth of virulent Pst DC3000 was increased in mkk3 knockout plants and decreased in MKK3-overexpressing plants. Moreover, MKK3 overexpression lines showed increased expression of several PR genes. By yeast two-hybrid analysis, coimmunoprecipitation, and protein kinase assays, MKK3 was revealed to be an upstream activator of the group C MAPKs MPK1, MPK2, MPK7, and MPK14. Flagellin-derived flg22 peptide strongly activated MPK6 but resulted in poor activation of MPK7. By contrast, MPK6 and MPK7 were both activated by H 2 O 2 , but only MPK7 activation was enhanced by MKK3. In agreement with the notion that MKK3 regulates the expression of PR genes, ProPR1:GUS expression was strongly enhanced by coexpression of MKK3-MPK7. Our results reveal that the MKK3 pathway plays a role in pathogen defense and further underscore the importance and complexity of MAPK signaling in plant stress responses.

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Section: Discussionmentioning

confidence: 77%

The Arabidopsis Mitogen-Activated Protein Kinase Kinase MKK3 Is Upstream of Group C Mitogen-Activated Protein Kinases and Participates in Pathogen Signaling

et al. 2007

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“…It has been shown that the lesion propagation in O 3 damage is under hormonal control (Overmyer et al, 2000;Rao et al, 2000Rao et al, , 2002Moeder et al, 2002;Kanna et al, 2003). Both jasmonateinsensitive (jar1 and coi1) and ET-overproducing mutants (eto1 and eto2) are O 3 sensitive as a result of lesion propagation, whereas the ET-or SA-insensitive mutants (etr1, ein2, npr1, and NahG) are O 3 tolerant.…”

Section: Is Rcd1 An Ozone Sensitive or Hormone Mutant?mentioning

confidence: 99%

“…Both jasmonateinsensitive (jar1 and coi1) and ET-overproducing mutants (eto1 and eto2) are O 3 sensitive as a result of lesion propagation, whereas the ET-or SA-insensitive mutants (etr1, ein2, npr1, and NahG) are O 3 tolerant. Furthermore, the mutants and accessions that have first been described as O 3 sensitive have also turned out to be partially JA insensitive (oji1, rcd1, and Cvi-0) or ET overproducers (rcd1) (Overmyer et al, 2000;Rao et al, 2000;Kanna et al, 2003). Moreover, the endogenous ROS production triggered by O 3 is ET dependent (Overmyer et al, 2000;Moeder et al, 2002;Wohlgemuth et al, 2002;Kanna et al, 2003).…”

Section: Is Rcd1 An Ozone Sensitive or Hormone Mutant?mentioning

confidence: 99%

“…Furthermore, the mutants and accessions that have first been described as O 3 sensitive have also turned out to be partially JA insensitive (oji1, rcd1, and Cvi-0) or ET overproducers (rcd1) (Overmyer et al, 2000;Rao et al, 2000;Kanna et al, 2003). Moreover, the endogenous ROS production triggered by O 3 is ET dependent (Overmyer et al, 2000;Moeder et al, 2002;Wohlgemuth et al, 2002;Kanna et al, 2003). Thus, it is conceivable that in several instances O 3 sensitivity is a result of altered interactions between hormonal signaling pathways, which regulate the propagation of cell death through endogenous ROS production (Overmyer et al, 2003).…”

Section: Is Rcd1 An Ozone Sensitive or Hormone Mutant?mentioning

confidence: 99%

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Arabidopsis RADICAL-INDUCED CELL DEATH1 Belongs to the WWE Protein–Protein Interaction Domain Protein Family and Modulates Abscisic Acid, Ethylene, and Methyl Jasmonate Responses

et al. 2004

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Experiments with several Arabidopsis thaliana mutants have revealed a web of interactions between hormonal signaling. Here, we show that the Arabidopsis mutant radical-induced cell death1 (rcd1), although hypersensitive to apoplastic superoxide and ozone, is more resistant to chloroplastic superoxide formation, exhibits reduced sensitivity to abscisic acid, ethylene, and methyl jasmonate, and has altered expression of several hormonally regulated genes. Furthermore, rcd1 has higher stomatal conductance than the wild type. The rcd1-1 mutation was mapped to the gene At1g32230 where it disrupts an intron splice site resulting in a truncated protein. RCD1 belongs to the (ADP-ribosyl)transferase domain-containing subfamily of the WWE protein-protein interaction domain protein family. The results suggest that RCD1 could act as an integrative node in hormonal signaling and in the regulation of several stress-responsive genes.

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“…Jasmonate (JA) has been implicated in signaling in response to both biotic and abiotic stresses (Balbi and Devoto, 2008;Wasternack, 2006;2007). Wounding and pathogen infection, or exposure to ozone, cause endogenous JA accumulation (Howe, 2004;Kanna et al, 2003;Rao et al, 2000;Vijayan, et al, 1998;Wasternack, 2006). Heat stress also induces the JA signal transduction pathway (Clarke et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of Arabidopsis thaliana WRKY39 in Heat Stress

Zhou

Chen

et al. 2010

Molecules and Cells

206

127

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Arabidopsis thaliana WRKY39, a transcription factor that is induced by heat stress, is a member of the group II WRKY proteins and responds to both abiotic and biotic stress. Heat-treated seeds and plants of WRKY39 knock-down mutants had increased susceptibility to heat stress, showing reduced germination, decreased survival, and elevated electrolyte leakage compared with wild-type plants. In contrast, WRKY39 over-expressing plants exhibited enhanced thermotolerance compared with wild-type plants. RT-PCR and qRT-PCR analysis of wrky39 mutants and WRKY39 over-expressing plants identified putative genes regulated by WRKY39. Consistent with a role for WRKY39 in heat tolerance, the expression levels of salicylic acid (SA)-regulated PR1 and SA-related MBF1c genes were downregulated in wrky39 mutants. In contrast, over-expression of WRKY39 increased the expression of PR1 and MBF1c. The WRKY39 transcript was induced in response to treatment with SA or methyljasmonate. Analysis of heat stressinduced WRKY39 in defense signaling mutants, including coi1, ein2, and sid2, further indicated that WRKY39 was positively co-regulated by the SA and jasmonate (JA) signaling pathways. Together, these findings reveal that heat stress-induced WRKY39 positively regulates the cooperation between the SA-and JA-activated signaling pathways that mediate responses to heat stress.

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Isolation of an Ozone-Sensitive and Jasmonate-Semi-Insensitive Arabidopsis Mutant (oji1)

Cited by 54 publications

References 44 publications

The Arabidopsis Mitogen-Activated Protein Kinase Kinase MKK3 Is Upstream of Group C Mitogen-Activated Protein Kinases and Participates in Pathogen Signaling

The Arabidopsis Mitogen-Activated Protein Kinase Kinase MKK3 Is Upstream of Group C Mitogen-Activated Protein Kinases and Participates in Pathogen Signaling

Arabidopsis RADICAL-INDUCED CELL DEATH1 Belongs to the WWE Protein–Protein Interaction Domain Protein Family and Modulates Abscisic Acid, Ethylene, and Methyl Jasmonate Responses

Functional Characterization of Arabidopsis thaliana WRKY39 in Heat Stress

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