Isolation of an iridovirus-like agent from common frogs (Rana temporaria)

Drury, S. E. N.; Gough, R. E.; Cunningham, A. A.

doi:10.1136/vr.137.3.72

Cited by 55 publications

(41 citation statements)

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“…In addition, this bacterium is frequently isolated from aquatic species, with or without disease (Bollinger et al 1999). Taking into account the virus isolation, the immunohistochemical results and the ranavirus distribution in tadpoles, it was concluded that A. hydrophila was not significant to disease development, and could thus be considered a secondary agent or postmortem invader, as has been previously observed in frogs with ranavirus disease (Drury et al 1995, Cunningham et al 1996. The CPE we observed in EPC monolayers infected with filtrates of visceral tissue homogenates taken from diseased tadpoles and with passaged virus was consistent with that caused by iridoviruses isolated previously from fish and amphibians (Fraser et al 1993, Cunningham et al 1996, Jancovich et al 1997, Tapiovaara et al 1998).…”

Section: Discussionmentioning

confidence: 79%

Pathology, isolation and molecular characterisation of a ranavirus from the common midwife toad Alytes obstetricans on the Iberian Peninsula

Balseiro

Dalton²,

Cerro³

et al. 2009

Dis. Aquat. Org.

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We describe the pathology, isolation and characterisation of a virus responsible for an outbreak of a systemic haemorrhagic disease causing high mortality in tadpoles of the common midwife toad Alytes obstetricans in the 'Picos de Europa' National Park in northern Spain. The virus, provisionally designated as the common midwife toad virus (CMTV), was isolated from homogenates of visceral tissue from diseased toad tadpoles following inoculation on epithelioma papulosum cyprini (EPC) cells. Molecular characterisation of the virus, including sequence analysis of the DNA polymerase and major capsid protein genes, showed that the isolated virus was a ranavirus with marked sequence identity to other members of the genus Ranavirus. A rabbit antiserum raised against purified virions was prepared and used to definitively demonstrate systemic distribution of the virus in diseased tadpoles, indicating that the isolated virus was the primary pathogen.

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Section: Discussionmentioning

confidence: 79%

Pathology, isolation and molecular characterisation of a ranavirus from the common midwife toad Alytes obstetricans on the Iberian Peninsula

Balseiro

Dalton²,

Cerro³

et al. 2009

Dis. Aquat. Org.

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“…This virus was detected in captive animals in Australia (Speare and Smith 1992 ). About the same time, epizootic die-offs were being recorded in the southwestern USA and the UK (Collins et al 1988 ;Cunningham et al 1993 ), but it was not until the mid-1990s that the etiology of the disease was determined to be a ranavirus (Drury et al 1995 ;Cunningham et al 1996 ;Jancovich et al 1997 , Fig. 2 ).…”

Section: Ranaviruses Infecting Amphibiansmentioning

confidence: 99%

“…In Europe, the fi rst outbreaks of FV3-like viruses occurred in the southeastern UK in common frogs (Cunningham et al 1993(Cunningham et al , 1996Drury et al 1995 ). The emergence of these viruses in common frogs was followed quickly by their emergence in common toads ( Bufo bufo ; Hyatt et al 2000 ;Cunningham et al 2007 ).…”

Section: Frog Virusmentioning

confidence: 99%

Distribution and Host Range of Ranaviruses

et al. 2015

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“…For example, ranaviruses (RVs) have been isolated from amphibians in North America (6,18,24,34,35), Asia (27,66), Australia (56), and the United Kingdom (10,19), from fish (2,41,46), and from reptiles (3,14,30,37,42,43). In fact, ranaviruses are now considered agents of emerging infectious disease (9).…”

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confidence: 99%

Evidence for Multiple Recent Host Species Shifts among the Ranaviruses (FamilyIridoviridae)

Jancovich

Brémont

Touchman

et al. 2010

J Virol

121

123

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Members of the genus Ranavirus (family Iridoviridae) have been recognized as major viral pathogens of cold-blooded vertebrates. Ranaviruses have been associated with amphibians, fish, and reptiles. At this time, the relationships between ranavirus species are still unclear. Previous studies suggested that ranaviruses from salamanders are more closely related to ranaviruses from fish than they are to ranaviruses from other amphibians, such as frogs. Therefore, to gain a better understanding of the relationships among ranavirus isolates, the genome of epizootic hematopoietic necrosis virus (EHNV), an Australian fish pathogen, was sequenced. Our findings suggest that the ancestral ranavirus was a fish virus and that several recent host shifts have taken place, with subsequent speciation of viruses in their new hosts. The data suggesting several recent host shifts among ranavirus species increase concern that these pathogens of cold-blooded vertebrates may have the capacity to cross numerous poikilothermic species barriers and the potential to cause devastating disease in their new hosts.

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Isolation of an iridovirus-like agent from common frogs (Rana temporaria)

Cited by 55 publications

References 0 publications

Pathology, isolation and molecular characterisation of a ranavirus from the common midwife toad Alytes obstetricans on the Iberian Peninsula

Pathology, isolation and molecular characterisation of a ranavirus from the common midwife toad Alytes obstetricans on the Iberian Peninsula

Distribution and Host Range of Ranaviruses

Evidence for Multiple Recent Host Species Shifts among the Ranaviruses (FamilyIridoviridae)

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