Isolation of an extracellular proteinase (Keratinase) from<i>Microsporum canis</i>

Takiuchi, Iwao; Higuchi, Daisuke; Sei, Yoshihiro; Koga, Miho

doi:10.1080/00362178285380411

Cited by 96 publications

(35 citation statements)

References 16 publications

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“…A T. mentagrophytes 48 kDa protease (Yu et al, 1968) may be meMep4 and a 45 kDa M. canis protease (Takiuchi et al, 1982(Takiuchi et al, , 1984 is probably caMep3 or caMep4. In contrast, the T. rubrum keratinolytic protease active as a dimer with subunits of 44 kDa (Asahi et al, 1985) and the T. mentagrophytes 41 kDa protease (Tsuboi et al, 1989) are not fungalysins but serine proteases as attested by the effect of different inhibitors.…”

Section: Discussionmentioning

confidence: 99%

Multiplication of an ancestral gene encoding secreted fungalysin preceded species differentiation in the dermatophytes Trichophyton and Microsporum

et al. 2004

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Dermatophytes are human and animal pathogenic fungi which cause cutaneous infections and grow exclusively in the stratum corneum, nails and hair. In a culture medium containing soy proteins as sole nitrogen source a substantial proteolytic activity was secreted by Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. This proteolytic activity was 55-75 % inhibited by o-phenanthroline, attesting that metalloproteases were secreted by all three species. Using a consensus probe constructed on previously characterized genes encoding metalloproteases (MEP) of the M36 fungalysin family in Aspergillus fumigatus, Aspergillus oryzae and M. canis, a five-member MEP family was isolated from genomic libraries of T. rubrum, T. mentagrophytes and M. canis. A phylogenetic analysis of genomic and protein sequences revealed a robust tree consisting of five main clades, each of them including a MEP sequence type from each dermatophyte species. Each MEP type was remarkably conserved across species (72-97 % amino acid sequence identity). The tree topology clearly indicated that the multiplication of MEP genes in dermatophytes occurred prior to species divergence. In culture medium containing soy proteins as a sole nitrogen source secreted Meps accounted for 19-36 % of total secreted protein extracts; characterization of protein bands by proteolysis and mass spectrometry revealed that the three dermatophyte species secreted two Meps (Mep3 and Mep4) encoded by orthologous genes.

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Section: Discussionmentioning

confidence: 99%

Multiplication of an ancestral gene encoding secreted fungalysin preceded species differentiation in the dermatophytes Trichophyton and Microsporum

et al. 2004

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“…In dermatophyte and fungal infections, there is clear evidence suggesting that proteinases produced by these organisms play an important role in the pathogenesis of tissue invasion (2,11,21,25,(28)(29)(30)(31). Although H. toruloidea causes a similar infection, no proteinase from this organism has been reported.…”

mentioning

confidence: 99%

Purification and characterization of an extracellular proteinase from hendersonula toruloidea

Rojanavanich¹,

Yoshiike²,

Tsuboi³

et al. 1990

Journal of Dermatological Science

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“…The stratum corneum is made up of keratin. The dermatophytes produce keratinases, which hydrolyze this substance and facilitate the growth of these organisms in the stratum corneum (Takiuchi et al, 1982). Thus, the dermatophyte infections are limited to superficial locations of the skin.…”

Section: Overviewmentioning

confidence: 99%

Immune Surveillance against Dermatophyte Infection

Koga

2005

Fungal Immunology

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Isolation of an extracellular proteinase (Keratinase) fromMicrosporum canis

Cited by 96 publications

References 16 publications

Multiplication of an ancestral gene encoding secreted fungalysin preceded species differentiation in the dermatophytes Trichophyton and Microsporum

Multiplication of an ancestral gene encoding secreted fungalysin preceded species differentiation in the dermatophytes Trichophyton and Microsporum

Purification and characterization of an extracellular proteinase from hendersonula toruloidea

Immune Surveillance against Dermatophyte Infection

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