Isolation of an Extensively Drug-Resistant Pseudomonas aeruginosa
            <i>exoS</i>
            <sup>+</sup>
            /O4 Strain Belonging to the “High-Risk” Clone ST654 and Coproducer of NDM-1 and the Novel VIM-80

Opazo-Capurro, Andrés; Morales-León, Felipe; Jerez, Christian; Olivares-Pacheco, Jorge; Alcalde-Rico, Manuel; González-Muñoz, Paulina; Bello-Toledo, Helia; Cardenas-Arias, Adriana; Esposito, Fernanda; Lincopán, Nilton; Illesca, Vijna; González-Rocha, Gerardo

doi:10.1128/spectrum.01439-22

Cited by 3 publications

(1 citation statement)

References 8 publications

(16 reference statements)

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“…NDM-1 has been identified mostly in Escherichia coli , Klebsiella pneumoniae , and, to a lesser extent, Acinetobacter and Pseudomonas . P. aeruginosa strain carriers of the bla NDM-1 -encoding gene have been found to circulate in various countries internationally, belonging in different Sequence Types (STs): ST773 in Libya; STs 38, 773, 235, 357, and 654 in Asia; STs 235 and 654 in Serbia; ST 773 in Korea; ST654 in Chile; ST274 in Ethiopia; ST1966 in China; and ST 308 in Singapore and Malaysia [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

First Detection and Molecular Characterization of Pseudomonas aeruginosa blaNDM-1 ST308 in Greece

Tsilipounidaki,

Gkountinoudis,

Florou

et al. 2023

Microorganisms

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The objective of the present study is to report the detection and the molecular characterization of nine blaNDM-1-positive Pseudomonas aeruginosa isolates, all of which belonged to the epidemic high-risk international clone ST308, and all were isolated from patients in a tertiary care hospital in Central Greece from May to July 2023.The isolates were characterized by whole genome sequencing to obtain multi-locus sequencing typing (MLST) and identify the blaNDM1-environment and resistome and virulence genes content. In silico MLST analysis showed that all isolates belonged to the high-risk ST308 international clone. All strains possessed 22 different genes, encoding resistance to various antimicrobial agents. Whole genome sequencing revealed that the blaNDM-1 was chromosomally located within the integrative and conjugative element ICETn43716385 and that it was part of one cassette along with two other resistance genes, floR and msrE. Two additional resistance cassettes were also found in the genome, which included the arrays of aph(6)-Id, aph(3″)-Ib, floR, sul2 and aadA10, qnrVC1, aac(3)-Id, dfrB5, aac(6′)-II. Additionally, the strains possessed various virulence genes, e.g., aprA, exoU, lasA, lasB, toxA, and estA. All of the isolates shared identical genomes, which showed 98% similarity with the P. aeruginosa ST308 genome (acc. no CP020703), previously reported from Singapore. To our knowledge, this is the first report of ST308 blaNDM-1-positive P. aeruginosa isolation in Europe, which indicates the transmission dynamics of this high-risk clone.

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Section: Introductionmentioning

confidence: 99%

First Detection and Molecular Characterization of Pseudomonas aeruginosa blaNDM-1 ST308 in Greece

Tsilipounidaki,

Gkountinoudis,

Florou

et al. 2023

Microorganisms

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Metallo-beta-lactamase blaNDM-1 in extremely drug-resistant high-risk Pseudomonas aeruginosa clone ST235 and non–high-risk clone ST2407 in Brazil

Morgado,

Freitas,

Rodrigues

et al. 2024

Journal of Global Antimicrobial Resistance

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Genomic and Phylogenomic Characterization of Carbapenem-resistant Pseudomonas aeruginosa ‘High-risk’ Clone O4/ExoS+/ST654 Circulating in Chilean Hospitals

Opazo-Capurro,

Aguilar-Vera,

González-Muñoz

et al. 2024

Journal of Global Antimicrobial Resistance

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Isolation of an Extensively Drug-Resistant Pseudomonas aeruginosa exoS ⁺ /O4 Strain Belonging to the “High-Risk” Clone ST654 and Coproducer of NDM-1 and the Novel VIM-80

Abstract: The aim of this study was to investigate the genomic features of an extensively drug-resistant (XDR) Pseudomonas aeruginosa isolate (P-469) emerging in Chile. Antibiotic susceptibility was determined by disk diffusion and “colistin agar” test.

Cited by 3 publications

References 8 publications

First Detection and Molecular Characterization of Pseudomonas aeruginosa blaNDM-1 ST308 in Greece

First Detection and Molecular Characterization of Pseudomonas aeruginosa blaNDM-1 ST308 in Greece

Metallo-beta-lactamase blaNDM-1 in extremely drug-resistant high-risk Pseudomonas aeruginosa clone ST235 and non–high-risk clone ST2407 in Brazil

Genomic and Phylogenomic Characterization of Carbapenem-resistant Pseudomonas aeruginosa ‘High-risk’ Clone O4/ExoS+/ST654 Circulating in Chilean Hospitals

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Isolation of an Extensively Drug-Resistant Pseudomonas aeruginosa exoS + /O4 Strain Belonging to the “High-Risk” Clone ST654 and Coproducer of NDM-1 and the Novel VIM-80

Abstract: The aim of this study was to investigate the genomic features of an extensively drug-resistant (XDR) Pseudomonas aeruginosa isolate (P-469) emerging in Chile. Antibiotic susceptibility was determined by disk diffusion and “colistin agar” test.

Cited by 3 publications

References 8 publications

First Detection and Molecular Characterization of Pseudomonas aeruginosa blaNDM-1 ST308 in Greece

First Detection and Molecular Characterization of Pseudomonas aeruginosa blaNDM-1 ST308 in Greece

Metallo-beta-lactamase blaNDM-1 in extremely drug-resistant high-risk Pseudomonas aeruginosa clone ST235 and non–high-risk clone ST2407 in Brazil

Genomic and Phylogenomic Characterization of Carbapenem-resistant Pseudomonas aeruginosa ‘High-risk’ Clone O4/ExoS+/ST654 Circulating in Chilean Hospitals

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Isolation of an Extensively Drug-Resistant Pseudomonas aeruginosa exoS ⁺ /O4 Strain Belonging to the “High-Risk” Clone ST654 and Coproducer of NDM-1 and the Novel VIM-80