Isolation of an exopolysaccharide-producing Streptococcus thermophilus from Algerian raw cow milk

Bennama, Rabha; Fernández, María; Ladero, Víctor; Álvarez, Miguel A.; Nadra, Rechidi-Sidhoum; Bensoltane, Ahmed

doi:10.1007/s00217-011-1620-8

Cited by 16 publications

(12 citation statements)

References 23 publications

(1 reference statement)

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“…5). In fact, the capsule formation in this strain was already described by Bennama et al [11]. The influence of this type of capsular EPS on the texture of fermented dairy products was confirmed by many authors [4,22].…”

Section: International Journal Of Bioscience Biochemistry Andsupporting

confidence: 76%

“…While at 37°C, a firm texture has characterized the samples of fermented skimmed milk. These textural characteristics could be related to the production of two types of EPS, previously observed in this strain [11]. Ever and about the texture, the microscopic observations performed on samples taken from fermented substrates showed the presence of capsule in this strain (Fig.…”

Section: International Journal Of Bioscience Biochemistry Andsupporting

confidence: 71%

“…Strain, Media and Growth Conditions S. thermophilus BN1, a strain isolated from raw milk [11] was grown in M17 supplemented with lactose (2% w/v) (LM17) at 42ºC under anaerobic conditions. EPS production by this strain was evaluated using as substrates: skimmed milk, whole milk, (Candia, Algeria) and cheese whey collected from the cheese factory "Sidi Saâda" (Relizane, Algeria).…”

Section: Methodsmentioning

confidence: 99%

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Effect of Some Fermentation Substrates and Growth Temperature on Exopolysaccharide Production by Streptococcus Thermophilus BN1

Bennama¹,

Nadra²,

Bensoltane³

2012

IJBBB

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Abstract-The purpose of this research was to evaluate, the effect of incubation temperature and some substrates on exopolysaccharides (EPS) production. The S. thermophilus BN1 an EPS-producer strain isolated from cow milk was examined in this study. Skimmed milk, whole milk and cheese whey, were selected as culture media and two different temperatures were also tested. The EPS produced in the different studied conditions was purified and quantified. The strain BN1 was able to produce EPS in all established conditions. However, significant differences were observed in the amounts of produced EPS. The strain has been shown to produce high amounts of EPS at 37°C compared to 42°C (P<0.05). In skimmed milk at 37°C, the EPS amounts reached 548 PDM mg l-1, followed by 375 PDM mg l-1 and 325 PDM mg l-1 respectively in whey and whole milk. In addition, a slight significant difference was noted on the biomass, pH and lactic acid values obtained from the three fermented substrates (p <0.05). The present results demonstrate that suboptimal growth temperature (37°C) had a significant effect on the EPS production by S. thermophilus BN1.

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Section: International Journal Of Bioscience Biochemistry Andsupporting

confidence: 76%

Section: International Journal Of Bioscience Biochemistry Andsupporting

confidence: 71%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Some Fermentation Substrates and Growth Temperature on Exopolysaccharide Production by Streptococcus Thermophilus BN1

Bennama¹,

Nadra²,

Bensoltane³

2012

IJBBB

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“…They may also be recovered by the process of precipitation after treatment with organic solvents soluble in water. The most commonly used are ethanol, acetone, methanol, and isopropanol (CERNING et al, 1994;MOREIRA;PINO;VENDRUSCOLO, 2003;GÓMEZ, 2006;BENNAMA et al, 2012). Extracellular microbial biopolymers have been widely used in several industry sectors adding value to various products, especially those food products, to improve their rheological characteristics.…”

Section: Resultsmentioning

confidence: 99%

An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation

et al. 2012

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The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs, which was confirmed by the formation of a precipitate when part of this colony was mixed with absolute alcohol. The established conditions for obtaining a high number of isolates producing EPS were 10% sucrose, pH 7.5 and 28 ºC. This method proved to be effective and economical because several strains could be tested on the same plate, with immediate confirmation.

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“…Lactic acid bacteria (LAB) are generally regarded as safe, gram-positive microorganisms [1] that play an essential role in the industrial production of various products and are excellent producers of exopolysaccharides (EPS). As bacteria belonging to this group are harmless to human and animal health [2] therefore investing in the production of EPS for industrial use including food industry has become particularly interesting.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Various Process Parameters Using Response Surface Methodology for Exopolysaccharide Production from a Novel Strain Pediococcus acidilactici KM0 (Accession Number KX671557) Isolated from Milk Cream

Sharma¹,

Sharma²,

Bajwa³

et al. 2017

IJERMT

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Abstractediococcus acidilactici KM0 (accession number KX671557) isolated from milk cream was optimized by response surface methodology by using the Design Of Experiments (DOE) to evaluate the interactive effects of the process parameters i.e. incubation time (h), temperature (•C), pH, carbon concentration, nitrogen concentration production for the production of EPS . Maximum EPS production of 32.64 mg/ml observed after 24 h incubation at 35 •C using 1.50 % and 3% of carbon and nitrogen concentration respectively, at pH 6.5.

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Isolation of an exopolysaccharide-producing Streptococcus thermophilus from Algerian raw cow milk

Cited by 16 publications

References 23 publications

Effect of Some Fermentation Substrates and Growth Temperature on Exopolysaccharide Production by Streptococcus Thermophilus BN1

Effect of Some Fermentation Substrates and Growth Temperature on Exopolysaccharide Production by Streptococcus Thermophilus BN1

An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation

Optimization of Various Process Parameters Using Response Surface Methodology for Exopolysaccharide Production from a Novel Strain Pediococcus acidilactici KM0 (Accession Number KX671557) Isolated from Milk Cream

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