Isolation of an Escherichia coli mutant defective in cytochrome biosynthesis

Kajie, Shin-ichi

doi:10.1016/0378-1097(84)90334-3

Cited by 1 publication

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References 14 publications

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“…(14). We tested our originalfdnA isolate for aerobic growth on succinate minimal medium, and it was unable to grow (data not shown), although it grew as well as the wild type did on glucose minimal medium.…”

Section: Iouil O-olt2 (+Pmsf)mentioning

confidence: 99%

Formate-nitrate respiration in Salmonella typhimurium: studies of two rha-linked fdn genes

et al. 1988

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Localized mutagenesis was used to obtain rha-linked mutations in Salmonella typhimurium, resulting in defects in the nitrate reductase-linked formate dehydrogenase (FDHN). Thefdn mutants obtained fell into two groups which differed in several respects. Group I isolates lacked FDHN activity under all conditions examined and exhibited wild-type levels of the hydrogenase-linked formate dehydrogenase (FDHH). Group II isolates appeared defective in FDHN only when freshly prepared extracts were assayed; restoration of both FDHN and formate-nitrate reduction activity occurred on incubation of extracts for 2 to 3 h. Protease inhibitors prevented restoration. Group II isolates were also characterized by a conditional FDHH activity; this activity was absent unless the growth medium designed to optimize wild-type FDHH was altered either by lowering glucose concentration or by adding thiosulfate. Cotransduction offdn with rha ranged from 4 to 22% for the group I isolates and from 20 to 40% for the group II isolates. Temperature-sensitive isolates from both groups synthesized FDHN activity with altered thermostability. In vitro complementation occurred in mixed extracts of amber mutants of the two respective classes. The results are consistent with two distinct rha-linkedfdn genes, for which we suggest using the designations fdnB (group I) and fdnC (group II).

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Section: Iouil O-olt2 (+Pmsf)mentioning

confidence: 99%