Isolation of an anti-tumor polysaccharide from Auricularia polytricha (jew’s ear) and its effects on macrophage activation

In vivo immunostimulatory and tumor-inhibitory activities of polysaccharides isolated from solid-state-cultured Trametes robiniophila Murrill

“…Macrophages play an important role in the defense mechanism of killing tumor cell, and they can be stimulated by various agents including LPS (Kang et al 2002), and NO, which are usually considered as significant indicator for macrophage activation, and has been recognized as an important messenger in immunostimulation against tumor cells (Yu et al 2009). Therefore, we detected if PST could activate macrophage through its phagocytosis ability and endogenous NO production.…”

“…Preparation of thioglycollate-eliciting peritoneal macrophages and phagocytosis assay Macrophages phagocytosis was measured by neutral red uptake method as described (Yu et al 2009). Each animal was injected intraperitoneally with 2 ml of 3% thioglycollate 4 days before the last treatment.…”

Structure characterization and antitumor activity of an α β-glucan polysaccharide from Auricularia polytricha

“…Recently, some polysaccharides extracted from A. polytricha have been reported to possess anticancer activities, and the antitumor effects seem to be related to their structural features. For example, a β-(1→3)-linked backbone seems essential (Ma, Wang, Zhang, Zhang, & Ding, 2010;Yu et al, 2009), and β-(1→6)-linked side chains of glucose residues increase antitumor activity (Misaki et al, 1981). Ma, Wang, and Zhang (2008) reported a water soluble β-glucan with significant antitumor activity which was composed of a main chain of (1→4)-linked D-glucopyranosyl with branching points at O-6 of (1→6)-linked D-glucopyranosyl residues.…”

Immunostimulatory activity of the polysaccharides from Hyriopsis cumingii

“…Macrophages phagocytize extraneous materials to produce phagosome and then scavenge those extraneous matters by using some enzymes including acid phosphatase. Thus, the immune activity of macrophages can be represented indirectly by its devouring percentage [22,23]. The phagocytosis indices of crude HCPS and its purified fractions on devouring neutral red of peritoneal macrophages were all exceed 1.0 (Fig.…”