Isolation of Adipose-Derived Stromal Vascular Fraction Cells Using a Novel Point-of-Care Device: Cell Characterization and Review of the Literature

Abstract

The GID SVF-1 and GID SVF-2 are closed-system, disposable, scalable cellular isolation devices designed for isolating the human adipose-derived stromal vascular fraction (hSVF) from lipoaspirates at the clinical point of care. The purpose of this study was to characterize the device performance with respect to cell yield and viability of the hSVF, as well as the hSVF purity and cellular composition. Our results demonstrate that adipose-derived hSVF can be safely obtained using both devices and standardized methods, yielding cells that are free of bacterial contaminants as measured by endotoxin levels, Gram stain, and culture. In addition, they produce cellular preparations with compositions consistent with reported values within the peer-reviewed literature using similar methods.

A Novel Porcine Model for Future Studies of Cell-enriched Fat Grafting

“…This amount of SVF cells correlates to results from human studies in which the average SVF yield has been reported to range from 7.19 × 10 5 to 9.55 × 10 5 live cells/g adipose tissue. 32 – 34 In addition, similar to previous human studies, we demonstrated a significantly higher SVF yield from adipose tissue harvested by surgical excision compared with liposuction, 35 – 37 and a higher density of SVF cells in the lipoaspirate from the abdomen compared with the back. 35 , 38 , 39 …”

Adipose tissue stem cells in regenerative medicine

“…Adipose tissue adult stem cells, termed stem cells or mesenchymal stem cells (MSCs), can be harvested through surgery and direct excision, liposuction in the trunk and extremities as well as the Coleman technique for fat tissue transplantation and remodelling. This technique has shown a higher yield of viable adipocytes than conventional liposuction [ 1 , 17 , 18 ]. In liposuction, the unwanted fat of a part of the body is removed by aspiration and injected in the areas where increased volume is required or to achieve a cosmetic improvement.…”

“…Stem cells can be classified by their origin as: (a) embryonic; (b) fetal; (c) adult and (d) induced pluripotent. The classification can be simplified as embryonic and adult mesenchymal [ 1 ].…”

“…It is a tissue that originates in the mesoderm and is formed by adipocytes and a fraction of stromal cells that include vascular smooth muscle cells, endothelial cells, fibroblasts, monocytes, macrophages, pre-adipocyte lymphocytes and ADSCs. ADSCs may undergo differentiation to mesodermal or trans-mesodermal lineages and give rise to cells that are naturally of ectodermal origin [ 1 ].…”

“…The procedure based on the separation of the vascular stroma contained in adipose tissue has allowed access to stem cells without resorting to embryonic tissue, facilitating its use in regenerative medicine [ 1 , 12 ]. The (CD34 +) ADSCs are those that can differentiate and form nonhematopoietic colonies, in turn they form a compartment in which various subpopulations are identified: pro endothelial (CD146 + / CD31 + / CD34 +); pericytes (CD146 + / CD31– / CD34–), a transient subpopulation (CD146 + / CD31– / CD34 +); and stromal cells that show a greater potential to form adipocytes (CD146– / CD31– / CD34 +) [ 15 ].…”