Isolation of acetylated bile acids from the sponge Siphonochalina fortis and DNA damage evaluation by the comet assay

Cano, Laura Patricia Patiño; Bartolotta, Susana; Casanova, Natalia A.; Siless, Gastón Ezequiel; Portmann, Erika; Schejter, Laura; Palermo, Jorge Alejandro; Carballo, Marta A.

doi:10.1016/j.steroids.2013.05.020

Cited by 10 publications

(4 citation statements)

References 23 publications

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“…Therefore, the remaining 5 candidates of acetylated BAs were putatively assigned as 3- O -acetyl-3β-UDCA/MDCA, 3- O -acetyl-UDCA, 3- O -acetyl-HDCA, 3- O -acetyl-3β-DCA, and 3- O -acetyl-CDCA (Figure B2). It should be noted that it is the first time for acetylated BAs to be discovered in mammals, and before this, only a few of them have been reported in sponges …”

Section: Resultsmentioning

confidence: 99%

Alternating Dual-Collision Energy Scanning Mass Spectrometry Approach: Discovery of Novel Microbial Bile-Acid Conjugates

Zhu

Wang

et al. 2022

Anal. Chem.

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Bile acids (BAs) are a type of gut microbiota–host cometabolites with abundant structural diversity, and they play critical roles in maintaining host–microbiota homeostasis. In this study, we developed a new N-(4-aminomethylphenyl) pyridinium (AMPP) derivatization-assisted alternating dual-collision energy scanning mass spectrometry (AMPP-dual-CE MS) method for the profiling of BAs derived from host–gut microbiota cometabolism in mice. Using the proposed method, we discovered two new types of amino acid conjugations (alanine conjugation and proline conjugation) and acetyl conjugation with host BAs, for the first time, from mouse intestine contents and feces. Additionally, we also determined and identified nine new leucine- and phenylalanine-conjugated BAs. These findings broaden our knowledge of the composition of the BA pool and provide insight into the mechanism of host–gut microbiota cometabolism of BAs.

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Section: Resultsmentioning

confidence: 99%

Alternating Dual-Collision Energy Scanning Mass Spectrometry Approach: Discovery of Novel Microbial Bile-Acid Conjugates

Zhu

Wang

et al. 2022

Anal. Chem.

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“…According to the results of synchrotron beam diffraction on the crystal variolin B ( Figure 2 ), an alkaloid with anticancer activity from the Antarctic sponge Kirckpatrickia variolosa binds DNA via the intercalative mode [ 34 ]. Acetylated bile acids from Siphonochalina fortis did not induce DNA breaks in human lymphocytes [ 61 ].…”

Section: Results Of Literature Searchmentioning

confidence: 99%

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

Sjakste

Gajski

2023

Toxins

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Envenomation by animal venoms remains a serious medical and social problem, especially in tropical countries. On the other hand, animal venoms are widely used as a source of biologically active compounds for the development of novel drugs. Numerous derivatives of animal venoms are already used in clinical practice. When analysing the mechanisms of action of animal venoms, attention is usually focused on the main target of the venom’s enzymes and peptides such as neurotoxic, cytotoxic or haemorrhagic effects. In the present review, we would like to draw attention to the “hidden” effects of animal venoms and their derivatives in regard to DNA damage and/or protection against DNA damage. Alkaloids and terpenoids isolated from sponges such as avarol, ingenamine G or variolin B manifest the capability to bind DNA in vitro and produce DNA breaks. Trabectidin, isolated from a sea squirt, also binds and damages DNA. A similar action is possible for peptides isolated from bee and wasp venoms such as mastoparan, melectin and melittin. However, DNA lesions produced by the crude venoms of jellyfish, scorpions, spiders and snakes arise as a consequence of cell membrane damage and the subsequent oxidative stress, whereas certain animal venoms or their components produce a genoprotective effect. Current research data point to the possibility of using animal venoms and their components in the development of various potential therapeutic agents; however, before their possible clinical use the route of injection, molecular target, mechanism of action, exact dosage, possible side effects and other fundamental parameters should be further investigated.

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“…Preliminary results demonstrated that sponges are also responsible for the majority of the organic and inorganic carbon fixation (Bergagna, ). Additionally, cnidarians and sponges have been shown to harbour natural marine products of interest for biotechnology development (Molinski, Dalisay, Lievens, & Saludes, ; Patiño Cano et al, , ; Rojo de Almeida et al, ), and constitute some of the in‐progress studies of the area with promising preliminary results (Prieto et al, ).…”

Section: Discussionmentioning

confidence: 99%

Benthic communities in the Southwest Atlantic Ocean: Conservation value of animal forests at the Burdwood Bank slope

Schejter

Genzano

Gaitán

et al. 2020

Aquatic Conservation

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The characterization of benthic communities at the Burdwood Bank slope (≥200 m depth) is provided, comprising data from the marine protected area (MPA) Namuncurá II (created in December 2018), as well as from some deep bathyal surrounding areas in the Southwest Atlantic Ocean. This information was acquired during two research cruises with RV Puerto Deseado (Argentina) during 2016 and 2017. Marine animal forests were detected in the study area: coral gardens, mainly composed of alcyonaceans, were detected in the south and west of Burdwood Bank (MPA Namuncurá II) and neighbouring areas, whereas pennatulacean aggregations were detected in the north‐west of the plateau. The three‐dimensional structures of live but also dead corals provide substrate and refuge to many associated species. Benthic communities dominated by sponges were also recorded between 200 and 300 m in depth. Six sites that meet the characteristics of vulnerable marine ecosystems (VMEs) are reported. Conservation strategies applied to scarcely known austral Argentinean waters led to the establishment of a pioneer MPA (Namuncurá I). Following the management plan that aimed to establish the biodiversity of Namuncurá I and neighbouring areas, the area of protection has now been enlarged to include the southern slope of the bank (currently Namuncurá II), where marine animal forests were detected, at depths below 200 m. The data presented here provide empirical evidence to support the conservation value of the region.

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Isolation of acetylated bile acids from the sponge Siphonochalina fortis and DNA damage evaluation by the comet assay

Cited by 10 publications

References 23 publications

Alternating Dual-Collision Energy Scanning Mass Spectrometry Approach: Discovery of Novel Microbial Bile-Acid Conjugates

Alternating Dual-Collision Energy Scanning Mass Spectrometry Approach: Discovery of Novel Microbial Bile-Acid Conjugates

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

Benthic communities in the Southwest Atlantic Ocean: Conservation value of animal forests at the Burdwood Bank slope

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