Isolation of a Viral Agent from Pseudocowpox Disease

Moscovici, C.; Cohen, Edward P.; Sanders, James E.; DeLong, S.S.

doi:10.1126/science.141.3584.915

Cited by 31 publications

(10 citation statements)

References 4 publications

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“…These morphologic properties are in keeping with those reported for the paravaccinia subgroup of viruses (4,14,15,21,24,27,29,30,31,32,34,38,39,40). The dimensions of strain 66/1456Sc virion (280 m~ • 160 m~) also fall within the size range of the paravaccinia viruses, namely, from 200 to 325 m~ in length and from 105 to 200 m~ in width (1,4,14,15,26,27,31,38,39). It is of interest that the size of the isolated particle as determined by filtration through gradocol membranes, is much greater than the value obtained by electron microscopy.…”

Section: Discussionsupporting

confidence: 89%

“…Thus its behavior in the presence of these lipid solvents is characteristic of the paravaceinia viruses (12,14,18,23,24,25,37). As with the latter, the isolate under study contains DNA (10,23,30), is sensitive to pH 3.0, is devoid of hemagglutinationhemadsorption activity (1,12,14,25,27,38), and lacks pathogenicity for rabbits, mice, and chicken embryos (8,14,16,18,22,27,38).…”

Section: Discussionmentioning

confidence: 97%

“…In contrast to the vaccinia virus, paravaccinia viruses are smaller, oval in shape, and have more superficial bands (34). They are responsible for the following diseases: contagious pustular dermatitis (CPD) or "orf" (1,4,15,29,30,34); bovine papula r stomatitis (BPS) (4,31,34,35,36,39,40); and pseudo-cowpox or milker's nodules (8,10,14,18,21,24,26,27,32). Additional strains have been isolated from the milk of cattle (12) and from vaccinia lymph (38).…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of a paravaccinia virus from cattle

Castrucci

McKeecher

Cilli

et al. 1970

Archiv f Virusforschung

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SummaryA strain of paravaccinia virus (66/1456Sc) was isolated from nasal flushings and pharyngeal swabbings collected from one calf involved in an outbreak of a respiratory disease syndrome which occurred in a group of 20 stabled cattle.The isolate appeared to be a lipovirus of the DNA type, pH 3 labile, heat sensitive, and not affected by either 2-(alpha-hydroxybenzyl)-benzimidazole in a concentration of 50 ~g per ml or by a 0.125~o solution of trypsin. The isolate multiplies in bovine embryo kidney (BEK) cell cultures, producing CPE characterized by the formation of "closed plaques" and intracytoplasmic inclusions. It possesses morphologic characteristics peculiar to those of the paravaccinia viruses, and dimensions of approximately 280 by 160 m~.The virus is devoid of hemagglutination or hemadsorption activity, and failed to infect sheep, rabbits, or mice, or to produce lesions on the CAM of fertile hens' eggs.Cattle inoculated intranasally, intravenously, or intramuscularly with the isolate, or by the intradermal route in the neck or muzzle, remained clinically healthy. However, when inoculated into the submucosa of calves, or intradermally into the teats of milking cows, the virus produced small ulcerative lesions in the former and erythematous nodules in the latter animals. Neutralizing antibodies were not produced by any of the animals injected with the agent, except by rabbits in one of three attempts.The virus under study was partially neutralized by antiserum to a strain of pseudo-cowpox virus, but no serologic crossing occurred between the isolate and antiserums to any of the following viruses: parainfluenza-3, vaccinia, bovine viral diarrhea, infectious bovine rhinotracheitis, pseudorabies, Shope fibroma, or sheep pox.The isolate reactivated heat-inactivated Lancy-Vaxina strain of vaccinia virus in CAM and BEK cell cultures. Studies of the isolate in cattle indicate that it was not responsible for the respiratory signs observed in the disease outbreak from which iV was isolated.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Characteristics of a paravaccinia virus from cattle

Castrucci

McKeecher

Cilli

et al. 1970

Archiv f Virusforschung

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“…The oncogene v-myb was originally defined by the chicken retroviruses AMV and E26 and is responsible for the transformation of myelomonocytic hematopoietic cells by these viruses (Moscovici and Gazzolo, 1982). The c-myb gene, from which v-myb is derived, is essential for the proliferation of hematopoietic precursor cells.…”

Section: Introductionmentioning

confidence: 99%

Functional antagonism between members of the myb family: B-myb inhibits v-myb-induced gene activation.

Foos¹,

Grimm²,

Klempnauer³

1992

The EMBO Journal

103

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The oncogene v‐myb and its cellular progenitor c‐myb encode nuclear, DNA binding phosphoproteins that control the expression of certain target genes in immature hematopoietic cells. Here, we report the isolation of a myb‐related chicken gene, chicken B‐myb. We show that expression of B‐myb, unlike that of c‐myb, is not restricted to hematopoietic cells, suggesting that B‐myb functions in a broader spectrum of cell types than c‐myb. We have identified the authentic chicken B‐myb protein as a nuclear protein of approximately 110 kDa. We show that the B‐myb protein specifically recognizes v‐myb binding sites in vitro and that binding is mediated by an N‐terminally located DNA binding domain. Although B‐myb protein recognizes myb binding sites, B‐myb fails to transactivate several myb‐responsive gene constructs as well as the endogenous myb‐responsive gene mim‐1. Instead, we find that B‐myb represses v‐myb‐ and c‐myb‐mediated activation of the mim‐1 gene, most likely by competing with other myb proteins for binding sites. Our results raise the possibility that B‐myb is an inhibitory member of the myb family.

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“…The retroviral oncogene v-myb, originally defined by the chicken retroviruses AMV and E26, transforms myelomonocytic hematopoietic cells in vivo and in vitro (Moscovici and Gazzolo, 1982). The progenitor of v-myb, c-myb, is expressed at high levels in immature cells of various hematopoietic lineages (Westin et al, 1982;Craig and Block, 1984;Gonda and Metcalf, 1984).…”

Section: Introductionmentioning

confidence: 99%

Estrogen-dependent alterations in differentiation state of myeloid cells caused by a v-myb/estrogen receptor fusion protein.

Burk

Klempnauer

1991

The EMBO Journal

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The oncogene v‐myb and its cellular progenitor c‐myb encode nuclear, DNA binding phosphoproteins that are thought to regulate the expression of myb‐responsive genes during myeloid differentiation. To identify such myb‐regulated genes, and to explore the mechanisms by which v‐myb affects their expression, we have established a conditional expression system for v‐myb. We have converted the v‐myb protein to an estrogen‐inducible transactivator by fusing the protein to the hormone binding domain of the human estrogen receptor. Expression of the chimeric protein in a chicken macrophage cell‐line causes estrogen‐dependent, reversible changes in the differentiation state as well as alterations in the gene expression program of the cells. We have used this estrogen‐dependent v‐myb expression system to identify a novel v‐myb regulated gene.

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Isolation of a Viral Agent from Pseudocowpox Disease

Cited by 31 publications

References 4 publications

Characteristics of a paravaccinia virus from cattle

Characteristics of a paravaccinia virus from cattle

Functional antagonism between members of the myb family: B-myb inhibits v-myb-induced gene activation.

Estrogen-dependent alterations in differentiation state of myeloid cells caused by a v-myb/estrogen receptor fusion protein.

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