Isolation of a type C RNA virus from an established human histiocytic lymphoma cell line.

Abstract

A type C RNA virus has been detected in the culture fluids of the SU-DHL-1 human histiocytic lymphoma cell line previously established in this laboratory. In electron micrographs, the virus closely resembled other typical mammalian type C RNA tumor viruses in size and morphology. Viral RNA-dependent DNA polymerase activity has been demonstrated in particles (densities of 1.15 and 1.22 g/ml) in the microsomal cytoplasmic fraction and in pellets of culture fluids. The enzyme is partially inhibited by antibodies to the RNAdependent DNA polyerases of simian sarcoma virus and RD-i 14 virus but not by antibody to the polymerase of murine leukemia virus, suggesting some degree of relatedness to type C viruses of subhuman primate origin. Typical syncytial microplaques were induced when SU-DHL-1 cells were cocultivated with rat XC cells. Although no focus formation was noted in similarly cocultivated mouse UC1-B cell cultures, the numbers of foci induced in rat embryo fibroblasts by murine sarcoma virus were significantly increased by coinfection with the virus from SU-DHL-1 cell culture fluids. No other evidence of infectivity, inducibility, or capacity for helper rescue of defective murine sarcoma virus genomes has been detected to date in cocultivation studies with a spectrum of fibroblastic and other nonlymphoid indicator cell lines of human and other species of origin. Type C RNA viruses have been identified as causative agents of leukemias, lymphomas, and sarcomas in avian, murine, feline, cavian, bovine, and subhuman primate species (cf. ref.

Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma

“…Most of these isolates are very closely related to previously isolated type C primate viruses (5)(6)(7), making interpretations of their origin difficult. The detection of retrovirus particles from some novel cell lines from patients with diffuse histocytic lymphoma has been recently described (8) and, although the DNA polymerase from these particles is also related to the reverse transcriptase (RT; RNA-dependent DNA nucleotidyltransferase) of type C primate viruses [simian sarcoma virus-gibbon ape leukemia virus (SSV-GALV) group], peptide maps of the purified DNA polymerases show significant differences between them (10,11).…”

“…Although some studies have suggested the presence of retrovirus information in some human tissues (3,4), the detection and isolation of complete, well-defined particles from humans has proven exceedingly difficult. The recent development of continuously growing cell lines from patients with various neoplastic diseases, and in some cases from normal tissues, has led to reports of retroviruses in some of them (5)(6)(7)(8)(9). Most of these isolates are very closely related to previously isolated type C primate viruses (5)(6)(7), making interpretations of their origin difficult.…”

In vitro susceptibilities of normal human skin fibroblasts to oncoviruses, and the decreased susceptibility to HSV of fibroblasts from untreated Hodgkin's patients

“…Both the aetiology and pathogenesis of Hodgkin's disease are unresolved, but it is usually considered primarily a disease of the lympho-reticuloendothelial cells, sometimes with secondary stimulation of growth of other cell types such as fibroblasts (Wahl et al, 1978;Kaplan et al 1977). If, indeed, the isolated HD fibroblasts differ from normal fibroblasts as indicated by our study, 2 more possibilities arise.…”

A Comparison of the Intracellular Precursor Polyproteins of Simian Sarcoma-associated Virus [SiSV(SiAV)] and Three Human Virus Isolates: HL23V, HEL12V and A1476V

“…Retroviruses have been isolated from several leukaemic patients by co-cultivation of tumour tissues with mammalian cell lines (Gallagher & Gallo, 1975;Panem et al, 1975;Smith et al, 1979;Gabelman et al, 1975;Kaplan et al, 1977). The best studied of these human virus isolates is HL23 virus (Gallagher & Gallo, 1975).…”