Isolation of a thermostable uricase-producing bacterium and study on its enzyme production conditions

Zhou, Xue-Lai; Ma, Xin; Sun, Guogui; Li, Xia; Guo, Kai

doi:10.1016/j.procbio.2005.05.002

Cited by 28 publications

(15 citation statements)

References 12 publications

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“…The highest dried cell weight (1.85 g) for uricase production was also observed at pH 6. Anderson and Vijayakumar [11] found pH 8.5 give highest production of uricase using Pseudomonas aeruginosa while Zhou et al [3] found that pH 7.5 was optimum pH for Microbacterium for production of uricase.…”

Section: A Effect Of Phmentioning

confidence: 99%

“…Although this enzyme is widely present in most vertebrates, it was found absent in human [3]. When the level of uric acid increases in blood over than the normal value it can cause renal failure and may contribute to a group of diseases such as gout, leukimia, toxemia of pregnancy, severe renal impairment and idiopathic calcium urate nephrolithiasis [4].…”

Section: Introductionmentioning

confidence: 99%

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Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus

Hatijah¹,

Ruhayu²

2013

IJBBB

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Abstract-Urate oxidase or uricase is an enzyme that catalyses the oxidation of uric acid to allantoin and plays important rule in purine metabolism. The first important application discovered for uricase was in clinical biochemistry as diagnosis reagent for measurement of uric acid in blood. The precipitation of uric acid can leading to gout symptom. The main purpose of this research is to optimize the culture condition for maximum uricase production by Aspergillus flavus. The parameters studied were pH (ranged pH4 to pH8), the sucrose concentration (10g/l to 50 g/l) and the agitation rate (100 to 300 rpm). The Aspergillus flavus were inoculated in yeast extract with sucrose and incubated for 24 hours at 200 rpm and 30 o C. The maximum enzyme activity obtained from the experiment is 0.03974 U/ml at pH 6, 200 rpm and 30 g/l sucrose concentration.

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Section: A Effect Of Phmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus

Hatijah¹,

Ruhayu²

2013

IJBBB

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“…At present, most enzymes applied in the clinical test are used in solution, and most proteins, including this enzyme, are relatively unstable when dissolved in aqueous solution. Therefore, for the enzymatic examination application, research was undertaken to search for a thermostable enzyme (Guo et al 2006;Huang et al 1998;Zhou et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of a thermostable uricase from Microbacterium sp. strain ZZJ4-1

Lou

Zhou

et al. 2007

World J Microbiol Biotechnol

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In order to study the properties of a thermostable uricase produced by Microbacterium sp. strain ZZJ4-1, the enzyme was purified by ammonium sulfate precipitation and DEAE-cellulose ion exchange, hydrophobic and molecular sieve chromatography. The molecular mass of the purified enzyme was estimated to be 34 kDa by SDS-PAGE. The enzyme was stable between pH 7.0 and 10.00. The optimal reaction temperature of the enzyme was 30°C at pH 8.5. The K m and K cat of the enzyme were 0.31 mM and 3.01 s -1 , respectively. Fe 3+ could enhance the enzyme activity, whereas Ag + , Hg 2+ , o-phenanthroline and SDS inhibited the activity of the enzyme considerably. After purification, the enzyme was purified 19.7-fold with 31% yield. As compared with uricases from other microbial sources, the purified enzyme showed excellent thermostability and other unique characteristics. The results of this work showed that strains of Microbacterium could be candidates for the production of a thermostable uricase, which has the potential clinical application in measurement of uric acid.

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“…Streptomyces exfoliatus UR10 was considered an excellent intracellular uricase producer. Most of the microbial uricase from Microbacterium ZZJ4-1, Proteus vulgaris 1753, P. vulgaris B317-C, Streptomyces graminofaciens, Streptomyces albidoflavus, and Streptomyces cyanogenus (7,26,38,39) was intracellular, and cell disruption was necessary to obtain the enzyme. However, in some microbial resources such as Bacillus fastidiosus (40) and Pseudomonas aeruginosa (41), extracellular uricase has been found without cell disruption.…”

Section: Discussionmentioning

confidence: 99%

Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes

Aly¹,

Tork²,

Al-Garni³

et al. 2013

Turk J Biol

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Uricase plays an important role in nitrogen metabolism and can be used medically as a diagnostic reagent. From soil, wastewater, and poultry waste samples collected in Jeddah, 49 bacterial isolates were obtained on either nutrient agar or starch nitrate agar. All the obtained isolates were screened on minimal medium containing 0.5% uric acid for uricase production. The most active bacterium (isolate UR10) produced about 0.5 U/mL of intracellular uricase and was identified as a species belonging to the genus Streptomyces using morphological, physiological, and biochemical characters. By 16S rDNA, it was identified as Streptomyces exfoliatus UR10. Maximum uricase production was obtained using medium 2 with 0.2% uric acid as an inducer, an initial pH of 6.5, and an incubation temperature of 37 °C at 100 rpm. At the end of the incubation period, the cells were collected and disturbed, and the uricase enzyme was precipitated by ammonium sulfate. The enzyme was purified using different column chromatography methods, and the molecular weight of the purified uricase was determined by SDS-PAGE electrophoresis. The optimum temperature for maximum uricase activity was 45 °C; the optimum pH was 8. Co 2+ , Ni 2+ , Zn 2+ , Cu 2+ , and Pb 2+ decreased the enzyme activity, whereas Ca 2+ , Mn 2+ , Mg 2+ , and Fe 2+ stimulated it. In conclusion, uricase was produced by Streptomyces in a medium containing uric acid as inducer, and this enzyme can be used to detect and quantify uric acid in urine and/or blood.

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Isolation of a thermostable uricase-producing bacterium and study on its enzyme production conditions

Cited by 28 publications

References 12 publications

Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus

Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus

Purification and characterization of a thermostable uricase from Microbacterium sp. strain ZZJ4-1

Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes

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