Isolation of a specific antigen with alkaline phosphatase activity from soluble extracts of<i>Paracoccidioides brasiliensis</i>

Yarzábal, L. A.; Andrieu, S; Bout, Daniel; Naquira, F.

doi:10.1080/00362177685190411

Cited by 23 publications

(13 citation statements)

References 9 publications

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“…Research on purification and clinical application of serological and skin tests has been done with antigens prepared with various citoplasmic and cell wall fractions of P. brasiliensis (3,10,14,15,24,35) and other dimorphic pathogenic fungi (13,16,29). Salvin & Smith (29) isolated an antigenic material from Histoplasma capsulatum, responsible for the induction of a delayed reaction in hamsters and humans.…”

Section: Antigenic Properties Of P Brasiliensis Cell Wallsmentioning

confidence: 99%

Paracoccidioides brasileensis: Cell wall structure and virulence

San-Blas

1977

Mycopathologia

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Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis or South American blastomycosis. Many aspects of the disease and its agent are unknown. One of the most important factors regarding the infection and the host-parasite relationships seems to be the fungal cell wall whose biochemical aspects are reviewed here. Biochemical studies, done mainly by Kanetsuna et al., (21,22), have demonstrated that the yeastlike (Y) and the mycelial (M) forms have chitin as a common polysaccharide, with alpha-1, 3-glucan in the Y form and beta-1, 3-glucan in the M form. These polysaccharides are fibrillar and determine to some degree the fungal shape. Moreover, an amorphous galactomannan is found in the cell wall of the M form. This compound is responsible for the antigenic properties of the cell wall (1). Recent studies (30-33) suggest that the cell wall does not possess a stable chemical structure but a rather changing one, as a function of the environment in which the fungus is grown. At the same time, the cell wall composition seems to correlate with the degree of virulence of the particular strain. From these observations it may be deduced that the constituent polysaccharides of P. brasiliensis cell wall, play an important role in the active protection of the fungus against the defensive mechanisms of the host.

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Section: Antigenic Properties Of P Brasiliensis Cell Wallsmentioning

confidence: 99%

Paracoccidioides brasileensis: Cell wall structure and virulence

San-Blas

1977

Mycopathologia

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“…Antiserum to antigen E/2 was prepared in rabbits according to the method described by Yarz~ibal et al [6]. Monospecificity of the serum was tested by gel immunodiffusion and bidimensional immunoelectrophoresis using crude soluble extracts of yeast and mycelial phases of P. brasiliensis as antigens.…”

Section: Serological Reagentsmentioning

confidence: 99%

“…Two of the specific antigens have been isolated and monovalent (monospecific) antisera have been produced against them [6,7]. The first, called E/l, showing alkaline phosphatase activity in immunoelectrophoregrams, is revealed only by a minor percentage of patients suffering mycologically confirmed paracoccidioidomycosis [6].…”

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confidence: 99%

“…The first, called E/l, showing alkaline phosphatase activity in immunoelectrophoregrams, is revealed only by a minor percentage of patients suffering mycologically confirmed paracoccidioidomycosis [6]. The second, named E/2, which manifests a weakly positive charge during agarose-gel electrophoresis at pH 8.2, showed immunological identity with the antigen of the band E [7], and reacted with all the human sera that formed this diagnostic immunoelectrophoretic band.…”

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confidence: 99%

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Sub-cellular localization of antigen E/2 ofParacoccidioides brasiliensisan immunoenzymatic electron microscopy study

Campo-Aasen¹,

Cabral²,

Yarzábal³

1980

Med Mycol

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The species-or genus-specific soluble antigen E/2 of Paracoccidioides brasiliensis was detected in yeast cells of the fungus by means of an immunoenzymatic technique. Peroxidase-labelled anti-E/2 immunoglobulins revealed the antigen as irregular vesicles or spots in the cytoplasm and as a fine coating on the plasma membrane of yeast cells of seven Venezuelan strains of P. brasiliensis. The antigen deposits seem to move to the periphery of the cells as the cultures age.Paracoccidioides brasiliensis, causal agent of paracoccidioidiomycosis, is an antigenically complex microorganism which possess at least 25 precipitant immunogens [4]. Early reports [5] have demonstrated the presence of species-or genus-specific antigens among such immunogens.Two of the specific antigens have been isolated and monovalent (monospecific) antisera have been produced against them [6,7]. The first, called E/l, showing alkaline phosphatase activity in immunoelectrophoregrams, is revealed only by a minor percentage of patients suffering mycologically confirmed paracoccidioidomycosis [6]. The second, named E/2, which manifests a weakly positive charge during agarose-gel electrophoresis at pH 8.2, showed immunological identity with the antigen of the band E [7], and reacted with all the human sera that formed this diagnostic immunoelectrophoretic band. Both mycelial and yeast phases of the fungus synthesized the substance. Antigen E/2 is therefore an important factor in the modulation of the host's immune response in paracoccidioidomycosis, and the determination of its cellular localization must be of interest in order to obtain fundamental information about the synthesis and release of this substance.The present studies concern the application of the immunoperoxidase technique to demonstrate the subcellular distribution of antigen E/2. An experimental monovalent (monospecific) antiserum anti-E/2 was conjugated with horseradish peroxidase and used to examine the yeast ceils of the fungus by electron microscopy. P. brasiliensis strains No. 1313P. brasiliensis strains No. , 2111 and 9570 from the Centro de Micologl'a M~dica de Caracas, Venezuela, were examined. All these MATERIALS AND METHODS Microorganisms

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“…It is only limited by the risk of false positive reactions, due to the common antigens shared by pathogenic fungi; also, the fact that relatively large quantities of reactants (150 microliter of antigen and serum) must be used, is another limitation. The recent experimental production of monospecific antisera against species-specific antigens of Aspergillus fumigatus (3) and Paracoccidioic[es brasiliensis (19) and the existence of micro-variants of the DDG (8) suggested to us the possibility of rendering the test more specific, by combining one of the microtechniques with the use of a reference monospecific antiserum.…”

mentioning

confidence: 99%