Isolation of a small molecule with anti–MRSA activity from a mangrove symbiont Streptomyces sp. PVRK–1 and its biomedical studies in Zebrafish embryos

Kannan, Rajaretinam Rajesh; Iniyan, Appadurai Muthamil; Prakash, Vincent Samuel Gnana

doi:10.1016/s2221-1691(11)60077-4

Cited by 14 publications

(11 citation statements)

References 37 publications

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“…Some other mutations of tubular heart and changes in cardiomorphology have been observed for both extracts after 12 h. Loss of α1β1-subunit is associated with small heart, diminished cardiac contraction, and absence of circulation[28], which affects the physiology and development of the heart but does not affect HBR. In the current study, no has and small mutations were detected in the small molecules from C. dactylon and S. acuta [18]. Bradycardia was observed following treatment with the C. dactylon extract and the LC 50 value was determined.…”

Section: Discussionmentioning

confidence: 48%

“…Embryos were raised in 10 mmol/L HEPES-buffered E3 medium in a dark incubator at 28°C until 60 hpf. Eggs containing dead or obviously weak embryos were removed[18],[19]. The study protocol was approved by the local institutional review board at the authors' affiliated institution and animal study was carried out in strict accordance with the established institutional guidelines.…”

Section: Methodsmentioning

confidence: 99%

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Cynodon dactylon and Sida acuta extracts impact on the function of the cardiovascular system in zebrafish embryos

Kannan

Vincent

2012

Journal of Biomedical Research

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The aim of the present study was to screen cardioactive herbs from Western Ghats of India. The heart beat rate (HBR) and blood flow during systole and diastole were tested in zebrafish embryos. We found that Cynodon dactylon (C. dactylon) induced increases in the HBR in zebrafish embryos with a HBR of (3.968±0.344) beats/s, which was significantly higher than that caused by betamethosone [(3.770±0.344) beats/s]. The EC50 value of C. dactylon was 3.738 µg/mL. The methanolic extract of Sida acuta (S. acuta) led to decreases in the HBR in zebrafish embryos [(1.877±0.079) beats/s], which was greater than that caused by nebivolol (positive control). The EC50 value of Sida acuta was 1.195 µg/mL. The untreated embryos had a HBR of (2.685±0.160) beats/s at 3 d post fertilization (dpf). The velocities of blood flow during the cardiac cycle were (2,291.667±72.169) µm/s for the control, (4,250±125.000) µm/s for C. dactylon and (1,083.333±72.169) µm/s for S. acuta. The LC50 values were 32.6 µg/mL for C. dactylon and 20.9 µg/mL for S. acuta. In addition, the extracts exhibited no chemical genetic effects in the drug dosage range tested. In conclusion, we developed an assay that can measure changes in cardiac function in response to herbal small molecules and determine the cardiogenic effects by microvideography.

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Section: Discussionmentioning

confidence: 48%

Section: Methodsmentioning

confidence: 99%

Cynodon dactylon and Sida acuta extracts impact on the function of the cardiovascular system in zebrafish embryos

Kannan

Vincent

2012

Journal of Biomedical Research

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“…The multidrug resistant phenotype is a characteristic feature of the MRSA strains and the antibiotic resistance is common for many classes of antibiotics such as aminoglycosides, macrolides, and fluoroquinolones. Nevertheless, all strains were sensitive to vancomycin (Awadalla et al, 2010;Kannan, 2011).…”

Section: Susceptibility Of Mrsa Isolates To Different Classes Of Antimentioning

confidence: 99%

Production of bioactive metabolites from different marine endophytic Streptomyces species and testing them against methicillin-resistant Staphylococcus aureus (MRSA) and cancer cell lines

El-Gendy¹,

Kamel²,

Hekal³

et al. 2018

bta

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The aim of the work was to identify Actinomycetes strains that are able to produce high levels of anti-MRSA metabolites with a potential use in curing resistant Staphylococcus aureus infections. Twenty-six endophytic Actinomycetes strains were isolated from various marine invertebrates living in two different marine ecosystems (the Mediterranean Sea and the Red Sea). Among them, Streptomyces sp. RS-9 (obtained from the soft coral Sarcophyton sp. from the Red Sea) and Streptomyces sp. MS-26 (obtained from the jellyfish derived from the Mediterranean Sea) demonstrated the highest antagonistic activity against 18 isolates of methicillin-resistant Staphylococcus aureus (MRSA) and 2 isolates of methicillin-resistant coagulase-negative Staphylococci (MRCoNS). The optimization of culture conditions, which includes the time course (at the 6 th and the 3 rd day of growth), the incubation temperature (27.5EC), pH (8.0 and 6.0), nutritional requirements comprising different metals, carbon (glucose and glycerol, respectively), and nitrogen (mixture of valine with peanut and a mixture of soybean with methionine and asparagines, respectively) sources for RS-9 and MS-26 strains, respectively, resulted in a 2.2-fold and 2.5-fold increase in the productivity of anti-MRSA metabolites. The GC/MS analysis proved the presence of different bioactive compounds in Streptomyces sp. RS-9 and Streptomyces sp. MS-26 extracts. Two pure compounds, A) 1-Methyl-2-acetyl-6-acetoxy-7-methoxy-(1,2,3,4-tetrahydroisoquinoline) and B)-4-(dimethylamino)-1,4,4d,5,12,12a-hexahydro-3,10,11,12a-tetrahydroxy-6-methyl-1,12-dioxo-,[4S-(4-α,4a,al]-(2-naphthacene carboxamide, which is a well-known antibiotic called anhydrotetracycline), were isolated from Streptomyces sp. MS-26. Those compounds possessed potent anti-MRSA activity against all the tested clinical MRSA and MRCoNS isolates. The minimum inhibition concentration (MIC) of the above two compounds ranged from 16 to 1024 μgml !1 and 4 to 128 μgml !1 , respectively. The minimum bactericidal concentration (MBC) ranged from 16 to 1024 μgml !1 and 8 to 128 μgml !1 , respectively with anticancer activity against colon cancer (HCT-16), liver cancer (HepG-2), and lung cancer (A-549) cell lines.

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“…The R. mucronata rhizome soil symbiont Streptomyces sp. PVRK1 was reported to produce anti-MRSA molecule [48]. Kathiresan and Selvam [22] characterized two bacteria to enhance the growth of mangrove seedlings from rhizosphere soil of R. mucronata.…”

Section: Chemical Screening Of Anti-mrsa Agentsmentioning

confidence: 99%

Characterization of Culturable Actinomycetes Associated with Halophytic Rhizosphere as Potential Source of Antibiotics

Iniyan

Kannan

Vincent

2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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The global-scale failure of bacterial drug resistance calls for an urgent need to develop novel antibiotics. Actinomycetes from marshy land or mangrove environment are relatively less explored for novel antibiotics against drug resistant pathogens. In the present work, the authors have characterized a few actinomycete isolates from rhizosphere region of two halophytes, Rhizophora mucronata and Achrostichum aureum which showed potential antagonistic property against Methicillin-resistant Staphylococcus aureus. 16S rDNA phylogenetic analyses revealed four clusters of actinomycetes belonging to the genera Streptomyces, Saccharopolyspora, Rhodococcus and Micromonospora. The bioactive metabolites were produced through agar plate fermentation and extracted with ethyl acetate. Thin layer chromatography bioautography confirmed the presence of anti-MRSA substances by 2,3,5-triphenyl-tetrazolium chloride based detection. The most active five compounds were purified through column chromatography and were found to be diverse molecules based on UV-Vis spectrum and reverse phase high performance liquid chromatography. Time kill experiments of partially purified active principles had proved the antibiotic potential against MRSA. Further activity-guided HPLC fractionation and characterization of these MRSA active actinomycetes are expected to reveal novel antibiotics with feasible potentials against broad spectrum of pathogens.

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Isolation of a small molecule with anti–MRSA activity from a mangrove symbiont Streptomyces sp. PVRK–1 and its biomedical studies in Zebrafish embryos

Cited by 14 publications

References 37 publications

Cynodon dactylon and Sida acuta extracts impact on the function of the cardiovascular system in zebrafish embryos

Cynodon dactylon and Sida acuta extracts impact on the function of the cardiovascular system in zebrafish embryos

Production of bioactive metabolites from different marine endophytic Streptomyces species and testing them against methicillin-resistant Staphylococcus aureus (MRSA) and cancer cell lines

Characterization of Culturable Actinomycetes Associated with Halophytic Rhizosphere as Potential Source of Antibiotics

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