Isolation of a retroviruslike sequence from the TL locus of the C57BL/10 murine major histocompatibility complex

Abstract

Two retroviruslike sequences have been isolated from the TL locus of the major histocompatibility complex of C57BL/10 mice. One sequence (TLev2) hybridizes only with probes derived from the pol region of the murine leukemia provirus AKR; the other sequence (TLevl) hybridizes with gag, pol, and env AKR region probes. This 9-kilobase endogenous, TL region-associated virus (TLevl) has been further characterized. The TLevl genome has been shown to contain murine leukemia virus-related sequences bounded by retroviruslike, VL30 long terminal repeats. Hybridization of TLevl-derived probes to mouse genomic digests reveals multiple copies which show distinct patterns compared with those observed with murine leukemia virus probes. The study of TLevl may prove significant with respect to the interaction of retroviral sequences within the genome, expression of genes within the TL locus, and polymorphisms within the major histocompatibility complex. * Corresponding author. of NZB xenotropic and an amphotropic virus were gifts of Raymond R. O'Neill, National Institutes of Health. An endogenous VL30 BVL-1 (26) clone was a gift of Micheal Getz, Mayo Clinic Foundation, Rochester, Minn.DNA preparation. (i) High-molecular-weight genomic DNA. Cells were washed and suspended in buffer containing 50 mM Tris (pH 7.5), 150 mM NaCl and 2 mM EDTA at a 296 on August 5, 2020 by guest

The Potential Roles of Endogenous Retroviruses in Autoimmunity

“…On tbe basis of the observation that T cells from MRL-lpr/lpr mice are resistant to antigen-induced apoptosis, abnormally low Fas expression has been proposed to impair Fas-mediated peripberal deletion of activated T cells, resulting in accumulation of aberrant T cells in MRLlpr/lpr mice (Russell et al 1993, Bossu et al 1993). Meruelo et al (1983Meruelo et al ( , 1984 demonstrated, by restriction fragment length polymorphism (RFLP) hybridization studies witb MuLV env probes, multiple integrations of ERV sequences in loci encoding lymphocyte differentiation antigens (LyI7 and Ly21 ) and major (TL region) and minor (H-3, 13, 28, 30, 38) bistocompatibility antigens in mice, some of which were cloned and sequenced and shown to be comprised of MuLV-related sequences (Pampeno & Meruelo 1986, Rossomando & Meruelo 1986. Surprisingly, hybridization of digested genomic DNA with one of the ERV sequences mapping in tbe minor histocompatibility antigen H-30 region, revealed that tbe related ERV insertions were located in the vicinity of 15 additional minor histocompatibility loci (Rossomando & Meruelo 1986).…”

Radiation Leukemia Virus and Its Effect on H‐2 Gene Expression

“…To date, however, this observation has not been made. Endogenous retroviral sequences have, however, been mapped both to the H-2 Tla region (Meruelo et al, 1984Pampeno & Meruelo, 1986 and to many minor histocompatibility loci (Meruelo er al., 1983;. The significance of these observations in the context of MHC regulation following transformation is not clear however.…”

“…Our group has long noted an association between endogenous viral sequences and genes encoding class I MHC antigens, minor histocompatibility antigens and several Ly antigens (Meruelo et al, 1983(Meruelo et al, ,1984Pampeno & Meruelo, 1986. Hybridization studies have shown that sequences hybridizing to xenotropic and ecotropic viral envelope probes are associated with Ly-17 on chromosome 1; H-3 and H-13 on chromosome 2; Ly-21 on chromosome 7; H-28 on chromosome 3; H-16 on chromosome 4; Thy-I on chromosome 9; H-30 on chromosome 15; and H-38 on chromosome 12.…”

“…Two retroviral-like sequences have also been mapped to the class I TL locus of the MHC (Meruelo et al, 1984;Pampeno & Meruelo, 1986. These two elements, TLeul and TLev2, have been characterized extensively and their examination has led to the extension of the TLb molecular map (Brown et al, , 1989.…”

“…4 are the relative locations of the TLev genomes as well as the nearby TLb genes of the C57BL/6 mouse. One sequence (TLev2) appears to be a defective viral genome while the other (TLevl) contains a 9-kb genome which has been shown to be comprised of murine leukemia virus-(MuLV) related sequences bounded by retrovirus-like VL30 long terminal repeats (Pampeno & Meruelo, 1986).…”