Isolation of a previously undescribed rickettsia from an aborted bovine fetus

Dilbeck, P. M.; Evermann, James F.; Crawford, Timothy B.; Ward, A. C. S.; Leathers, C. W.; Holland, C J; Mebus, Charles A.; Logan, Linda; Rurangirwa, F. R.; McGuire, Travis C.

doi:10.1128/jcm.28.4.814-816.1990

Cited by 86 publications

(52 citation statements)

References 4 publications

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“…Further, there is evidence for a potential pathogenicity of some environmental chlamydiae (Corsaro & Greub, 2006;Horn, 2008), such as Simkania negevensis (family Simkaniaceae) and Waddlia chondrophila (family Waddliaceae), which can thrive both in amebae and in mammalian cells, and which were associated with respiratory disease and/or miscarriage in humans (Dilbeck et al, 1990;Rurangirwa et al, 1999;Kahane et al, 2001;Henning et al, 2002;Friedman et al, 2003;Michel et al, 2004Michel et al, , 2005Kahane et al, 2007;Goy et al, 2008;Haider et al, 2008;Kahane et al, 2008;Baud & Greub, 2011;Kebbi-Beghdadi et al, 2011a). In contrast, the Parachlamydiaceae, including Parachlamydia acanthamoebae and Protochlamydia amoebophila, are primarily considered symbionts of amebae (Horn, 2008) and appear to have a limited capacity to grow in nonprotozoan host cells (Maurin et al, 2002;Greub et al, 2003;Collingro et al, 2005;Casson et al, 2006;Hayashi et al, 2010;Roger et al, 2010;Kebbi-Beghdadi et al, 2011b;Sixt et al, 2012).…”

Section: Environmental Chlamydiaea Hidden Diversitymentioning

confidence: 99%

Chlamydial metabolism revisited: interspecies metabolic variability and developmental stage-specific physiologic activities

Omsland¹,

Sixt²,

et al. 2014

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Chlamydiae are a group of obligate intracellular bacteria comprising important human and animal pathogens as well as symbionts of ubiquitous protists. They are characterized by a developmental cycle including two main morphologically and physiologically distinct stages, the replicating reticulate body and the infectious non-dividing elementary body. In this review we reconstruct the history of studies that have led to our current perception of chlamydial physiology, focusing on their energy and central carbon metabolism. We then compare the metabolic capabilities of pathogenic and environmental chlamydiae highlighting interspecies variability among the metabolically more flexible environmental strains. We discuss recent findings suggesting that chlamydiae may not live as energy parasites throughout the developmental cycle and that elementary bodies are not metabolically inert but exhibit metabolic activity under appropriate axenic conditions. The observed host-free metabolic activity of elementary bodies may reflect adequate recapitulation of the intracellular environment, but there is evidence that this activity is biologically relevant and required for extracellular survival and maintenance of infectivity. The recent discoveries call for a reconsideration of chlamydial metabolism and future in-depth analyses to better understand how species- and stage-specific differences in chlamydial physiology may affect virulence, tissue tropism, and host adaptation.

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Section: Environmental Chlamydiaea Hidden Diversitymentioning

confidence: 99%

Chlamydial metabolism revisited: interspecies metabolic variability and developmental stage-specific physiologic activities

Omsland¹,

Sixt²,

et al. 2014

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“…The role of free-living amoebae as reservoir for the novel chlamydiae may be important since amoebae can successfully colonize manmade water systems such as cooling towers, humidifiers, hospital water networks or drinking water distribution systems. It deserves particular attention since there is evidence for a potential pathogenicity of some environmental chlamydiae (Corsaro and Greub, 2006), such as Simkania negevensis, Waddlia chondrophila and Parachlamydia acanthamoebae, present in amoebae and mammalian cells, associated with respiratory disease and/or miscarriage in humans (Dilbeck et al, 1990;Kahane et al, 2008;Greub, 2009;Baud and Greub, 2011). However, the impact of environmental chlamydiae on public health is currently under debate (Corsaro and Greub, 2006;Lamoth et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Chlamydial seasonal dynamics and isolation of ‘Candidatus Neptunochlamydia vexilliferae’ from a Tyrrhenian coastal lake

Pizzetti

Schulz

Tyml

et al. 2015

Environmental Microbiology

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The Chlamydiae are a phylum of obligate intracellular bacteria comprising important human and animal pathogens, yet their occurrence in the environment, their phylogenetic diversity and their host range has been largely underestimated. We investigated the seasonality of environmental chlamydiae in a Tyrrhenian coastal lake. By catalysed reporter deposition fluorescence in situ hybridization, we quantified the small planktonic cells and detected a peak in the abundance of environmental chlamydiae in early autumn with up to 5.9 × 10(4) cells ml(-1) . Super-resolution microscopy improved the visualization and quantification of these bacteria and enabled the detection of pleomorphic chlamydial cells in their protist host directly in an environmental sample. To isolate environmental chlamydiae together with their host, we applied a high-throughput limited dilution approach and successfully recovered a Vexillifera sp., strain harbouring chlamydiae (93% 16S rRNA sequence identity to Simkania negevensis), tentatively named 'Candidatus Neptunochlamydia vexilliferae'. Transmission electron microscopy in combination with fluorescence in situ hybridization was used to prove the intracellular location of these bacteria representing the first strain of marine chlamydiae stably maintained alongside with their host in a laboratory culture. Taken together, this study contributes to a better understanding of the distribution and diversity of environmental chlamydiae in previously neglected marine environments.

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“…Вперше Waddlia chondrophila була виділена з аборт-плоду великої рогатої худоби у США в 1990 році (Dilbeck et al, 1990). Dilbeck P.M. et al, був підтверджений зв'язок між антитілами проти Waddlia chondrophila і абортами великої рогатої худоби.…”

Section: вступunclassified

PCR-test for identification and species differentiation of Waddlia chondrophila

et al. 2019

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In recent times, the number of species of chlamydia associated with a number of inflammatory diseases in animals has expanded considerably. Moreover, in addition to chlamydia, attention was attracted by Сhlamydia-like organisms, which are not only dangerous for animals, but also carry a zoonotic threat. Currently, the most studied Chlamydia-like organisms are Waddlia chondrophila and Parachlamydia acanthamoebae. They are associated with diseases of the reproductive and respiratory systems in cattle and humans. Considering the danger to animals and the zoonotic threat of chlamydia-like organisms along with the absence of tools to discover them in Ukraine, the aim of our work was to develop a PCR test for the identification and species differentiation of Waddlia chondrophila. Conservative 16S rRNA genes were chosen as target genes for developing a PCR test system for identifying Waddlia chondrophila. Primers were selected specifically to be able to create multiplex combinations with the previously developed PCR test for Parachlamydia acanthamoebae. Both primers were designed with the same physical characteristics to provide simultaneous amplification under the same conditions in single or multiplex PCR. For the specificity evaluation of the primers, a panel of following DNA samples was used: Waddlia chondrophila, Parachlamydia acanthamoebae, Chlamydia avium, Chlamydia pecorum, Chlamydia abortus, Chlamydia psittaci, Chlamydia suis, Chlamydia caviae, Clavochlamydia salmonicola, Piscichlamydia salmonis. PCR product of 88 base pairs (b.p.) was formed during amplification only when the Waddlia chondrophila control DNA was present in the sample, as was expected. The small size of the PCR product theoretically allows the use of this pair of oligonucleotide primers for real-time PCR tests. After testing on clinical samples, developed PCR test system for identifying and species differentiation of Waddlia chondrophila can be used by scientists for extensive monitoring, by veterinary medicine doctors to clarify the diagnosis, and might be introduced into the practice of laboratories of veterinary and humane medicine. 1 Полтавська державна аграрна академія, м. Полтава, Україна 2 Інститут свинарства і агропромислового виробництва НААН, м. Полтава, Україна Останнім часом кількість видів хламідій, пов'язаних з рядом запальних захворювань тварин, значно розшилась. Більш того, окрім хламідій, увагу привернули хламідіє-подібні організми, які не тільки є небезпечними для тварин, але й несуть зоонозну загрозу. Найбільш вивченими, на даний момент хламідіє-спорідненими організмами є Waddlia chondrophila та Parachlamydia acanthamoebae, їх пов'язують з захворюваннями репродуктивної та респіраторної систем великої рогатої худоби та людини. З огляду

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Isolation of a previously undescribed rickettsia from an aborted bovine fetus

Cited by 86 publications

References 4 publications

Chlamydial metabolism revisited: interspecies metabolic variability and developmental stage-specific physiologic activities

Chlamydial metabolism revisited: interspecies metabolic variability and developmental stage-specific physiologic activities

Chlamydial seasonal dynamics and isolation of ‘Candidatus Neptunochlamydia vexilliferae’ from a Tyrrhenian coastal lake

PCR-test for identification and species differentiation of Waddlia chondrophila

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