Isolation of a powerful antioxidant from Olea europaea fruit-mill waste: 3,4-Dihydroxyphenylglycol

Potential use of olive mill wastewater in the preparation of functional beverages: A review

“…Rodrìguez, Lama, Trujillo, Espartero, and Fernà ndez-Bolanos (2009) reported that a post-treatment of olive mill waste extract (aqueous extract) with high temperature (90°C) for 120 min, increased the concentration of 3,4-dihydroxyphenylglycol (DHPG) by 237%. DHPG is structurally similar to hydroxytyrosol but with an additional hydroxyl group and a higher antioxidant activity (Rodrìguez et al, 2009).…”

Recycling of Two-Phase Olive-Mill Cake “Alperujo” by Co-composting with Animal Manures

“…Apart from this, several alternatives have been proposed to minimize the environmental impact and to recycle ALP, such as the production of building ceramic material (de la Casa et al 2012), the obtaining and recovery of antioxidant compounds (Madrona et al 2009;Rodríguez et al 2009), the production of thermal or electrical energy, after the residual oil extraction (Arvanitoyannis, Kassaveti, and Stefanatos 2007), for soil bioremediation (Burgos et al 2010;de la Fuente et al 2011), and as organic fertilizer (Kavdir and Killi 2008; Lozano-García, Parras-Alcántara, and del Toro Carrillo de Albornoz 2011). However, most of these uses are generally very expensive and unable to completely solve the problem of these residues, and only the agricultural use is an economically and ecologically acceptable way to dispose of them.…”

Determination of 3,4-dihydroxyphenylglycol, hydroxytyrosol and tyrosol purified from olive oil by-products with HPLC in animal plasma and tissues

“…The first eluent was chosen to recover DHPG, avoiding potential degradation of this phenol in a consequent concentration step. We recently observed that using high concentrations of ethanol or methanol produces split peaks for DHPG during HPLC analysis (Rodríguez et al, 2009a(Rodríguez et al, , 2009b. Tissue extractions were carried out using two concentrations of ethanol (50% and 100%), and both gave similar recoveries for all three phenols (data not shown).…”

“…We chose 100% ethanol to extract DHPG, hydroxytyrosol and tyrosol from the tissues as it gave a cleaner extract with less interfering peaks close to the DHPG peak. We observed previously that DHPG is not stable in alkaline conditions, or when adding acid such as ascorbic acid to prevent the oxidation of phenols, as it degrades during the concentration process when the pH is out with the range of 6.0-6.5 (Rodríguez et al, 2009a(Rodríguez et al, , 2009b. Therefore, our method to extract phenols from plasma and tissues avoids the addition of any acid to the final solution, oxidation being prevented by use of a vacuum manifold and nitrogen during sample concentration.…”

“…Tyrosol was purified from the fraction that was eluted with water obtained from the first chromatographic column by an additional column of polystyrene based resins (Amberchrom Ò CG161). DHPG was also purified by a novel chromatographic system under patent in which water was the only eluent (Rodríguez, Lama, Trujillo, Espartero, & Fernández-Bolaños, 2009a, 2009b. In this case, three chromatographic steps were necessary to get the pure compound.…”