Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain

Gao, Yuhua; Li, Xiangchen; Zheng, Da Wei; Guan, Weijun; Ma, Yuehui

doi:10.3390/ijms16035990

Cited by 6 publications

(2 citation statements)

References 22 publications

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“…However, the applications are highly restricted due to various ethical concerns. Due to the challenges in neural stem cell extraction and the difficulty in mesenchymal stem cell (MSC) transdifferentiation, techniques for induced pluripotent stem cell (iPS) producing are developed via various reprogramming processes with specific transcription factors, such as Oct4, Sox2, Klf4, and c-Myc [127]. However, such therapy may face the challenges in safety concern for iPS induced teratomas [128], efficiency limitation in continuous stem cell delivery, and the invasive procedures in clinical practice to reach the central venous system.…”

Section: Neuroregenerative Therapymentioning

confidence: 99%

Intra-maxillary Molecular Releasing and its Application in the Assistance of Neurodegenerative Disease Therapeutics

J¹

2016

IJCTD

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Neurodegenerative disease (ND) is a complex concept involves multiple pathogenesis including neuronal demyelination, apoptosis under oxidative stress, neuroinflammation with further cortical and spinal atrophy, which may lead to cognition impairment and multiple neural dysfunctions. It may present as a chronic and irreversibly progressive disorder and shows the common pathological pictures among its various types including Alzheimer's disease (AD), Parkinson's disease (AD), Huntington's disease (HD), multiple sclerosis (MS), and amyotrophic lateral sclerosis (ALS). The symptoms often occur among the elderly above 50 years old and last from years to decades. Such facts lead ND as the highest priority to geriatrics and its related medical care. Due to its chronic progressively characteristics, regularly invasive procedure arrangements may face the difficulty and regarded as unbearable in the clinical practice. Unfortunately, current available therapy is mainly on symptom relief and the potential therapeutic targets may focus on the macromolecules including oligomer, peptides, or even functional proteins with the quaternary structure since multiple proteinopathy involvement in ND pathogenesis. Such macromolecular administrations cause the challenges in the routine drug delivery selections due to the impossibility of the traditional oral intake.Intra-maxillary molecular releasing is a newly drug delivery pathway via dental implant and may directly reach the inside bone marrow. It presents the advantages in long-term and continuous macromolecular releasing with relative painless process, which is appropriate for further applications in ND therapeutics. The stability of dental implant inside the oral cavity has also been proved for decades among more than millions around the world. However, such delivery pathway is currently under development and further interdisciplinary investigations are needed including therapeutics, pharmacokinetics, neurology, molecular biology, clinical dentistry, chemical engineering, automatics and further complementary metal-oxidesemiconductor (CMOS) technology to ensure the efficiency and prove the safety concern.

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Section: Neuroregenerative Therapymentioning

confidence: 99%

Intra-maxillary Molecular Releasing and its Application in the Assistance of Neurodegenerative Disease Therapeutics

J¹

2016

IJCTD

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“…Nestin, NSE, and GFAP were traditional makers of NSCs, neuron cells, and glial neurons, respectively (Hartfuss et al., 2001; Isgrò et al., 2015; Shaik et al., 2019). Oct4 and SOX2, two intracellular transcription factors, were used as indicators of stem cells (Gao et al., 2015; Lugert et al., 2010). We isolated NSCs from fetal mice and found that Ssd can induce the G0/G1 phase arrest and inhibit the differentiation, and Ssd can increase the stemness potential and thermotolerance of NSCs.…”

Section: Introductionmentioning

confidence: 99%

Saikosaponin‐d improved the stemness of mouse neural stem cells and increased their thermotolerance potential

Xiao

et al. 2021

Intl J of Devlp Neuroscience

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Purpose To investigate the effect of saikosaponin‐d (Ssd) on proliferation, differentiation, and stemness of neural stem cells (NSCs), and to observe whether Ssd has a protective effect on NSCs at medium‐high and high temperature. Materials and methods NSCs were extracted from 15‐day fetal mice. After subculture, Ssd treatment was performed. Cell cycle and apoptosis rate were detected by flow cytometry. Western Blot and immunofluorescence assay were used to detect the expression and spatial distribution of Nestin, NSE, GFAP, Oct4, and SOX2. Cell growth morphology was observed under a microscope; the concentration of extracellular lactate dehydrogenase (LDH) was determined by ELISA. Results Compared with the control group, the proportion of NSCs in the G0/G1 phase increased in the Ssd treatment group; on the contrary, the proportion in the G2/M phase significantly decreased. Microscopically, our results also suggested the sphere‐formation rate increased significantly. Besides, the percentage of dead cells in the Ssd group at 38.5, 40°C were reduced, and the level of LDH release was dropped. Conclusion Ssd improved the stemness of NSCs, inhibited their differentiation into neural cells, and reduced cell damage under high temperature. Therefore, we speculate that Ssd can improve the thermotolerance of NSCs and protect the nervous system of children with fever.

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Tissue-engineered nerve graft with tetramethylpyrazine for repair of sciatic nerve defects in rats

Xiang

Wei

Yang

et al. 2017

Neuroscience Letters

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Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain

Cited by 6 publications

References 22 publications

Intra-maxillary Molecular Releasing and its Application in the Assistance of Neurodegenerative Disease Therapeutics

Intra-maxillary Molecular Releasing and its Application in the Assistance of Neurodegenerative Disease Therapeutics

Saikosaponin‐d improved the stemness of mouse neural stem cells and increased their thermotolerance potential

Tissue-engineered nerve graft with tetramethylpyrazine for repair of sciatic nerve defects in rats

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