Isolation of a p15 polypeptide from bovine leukemia virus and detection of specific antibodies in leukemic cattle

Kaaden, Oskar‐Rüger; Frenzel, B.; Dietzschold, Bernhard; Weiland, Frank; Mussgay, M.

doi:10.1016/0042-6822(77)90475-5

Cited by 13 publications

(7 citation statements)

References 16 publications

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“…1490 and 567) animals reacted with p15 in the RIPA. The significance of this reaction is not clear but is in agreement with the results of other groups showing that BLV-pl5 reacts with the sera from animals of leukemic herds (Kaaden et al, 1977) and that 1/7 sera positive against gp51 in immunodiffusion also possessed anti-pl5 precipitating antibodies (Frenzel et al, 1977).…”

Section: Discussionsupporting

confidence: 83%

Spontaneous immune response of bovine leukemia‐virusinfected cattle against five different viral proteins

Deshayes

Lévy

Parodi

et al. 1980

Intl Journal of Cancer

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The sera from cattle exposed to bovine leukemia virus (BLV) have been studied by the radioimmunoprecipitation assay (RIPA) of disrupted virus proteins. All sera of animals with the adult form of lymphosarcoma precipitated four different viral proteins: gp51, gp35, p24 and p12. In contrast, the sera of five animals with the juvenile or thymic forms of bovine lymphosarcoma were completely devoid of precipitating activity against BLV proteins, confirming the absence of relationship between these rare malignant diseases and BLV infection. The sera of non-leukemic cattle from areas of high risk of exposure to BLV were either negative or positive to a lower degree than the sera of lymphosarcomatous cows. When positive they precipitated gp51, p24 and p12. Only one serum precipitated gp35. The difference was probably quantitative, the sera of leukemic animals having higher levels of precipitating activity against all the viral proteins. The anti-gp51 reactivity was in most cases the strongest, whatever the origin of the serum. In the rare sera which were positive in the classical anti-gp51 radioimmunoassay (RIA) but negative in the anti-p24 RIA, an anti-p24 activity was, however, detected by the RIPA. These results suggest that BLV-infected cows regularly produce antibodies reacting with four different viral proteins including minor viral components. In three cases with no other special features a low level of activity was detected against a fifth component: p15. There was no apparent correlation, however, between the antibody response and an hypothetical protection against BLV-induced disease.

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Section: Discussionsupporting

confidence: 83%

Spontaneous immune response of bovine leukemia‐virusinfected cattle against five different viral proteins

Deshayes

Lévy

Parodi

et al. 1980

Intl Journal of Cancer

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“…Bovine leukemia virus (BLV) has been implicated as an etiologic agent in lymphosarcoma of domestic cattle (Miller et al, 1969;Dutta ef al., 1969;Kawakami et al, 1969). Several laboratories are presently involved in the isolation and characterization of BLV antigens in attempts to develop diagnostic methods for detection of virus-infected animals (Devare et al, 1976;McDonald and Ferrer, 1976;Kaaden et al, 1977;Bex et at., 1979). Interest in this area of investigation has resulted, in part, from the fact that lymphosarcoma of domestic cattle provides a unique model system for studies on the role of oncornaviruses in tumours of their natural hosts.…”

mentioning

confidence: 99%

Bovine leukemia virus‐specific antibodies in french cattle. III. Prevalence of the BLV‐gp 51 radioimmunoassay for the detection of BLV‐infected animals

Lévy¹,

Deshayes²,

Parodi³

et al. 1980

Intl Journal of Cancer

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The control of the spread of BLV infection among cattle requires very sensitive methods of detection. The BLV-gp51 radioimmunoassay presents great advantages over the other serological methods, including the BLV-p24 radioimmunoassay. This is clear from studies of normal animals from the high-leukemia-incidence region which show that anti-gp51 antibodies reach higher titers than anti-p24 or may even exist alone. Moreover, a sequential survey reveals that the anti-gp antibodies appear earlier. On the other hand, it was not possible to detect a viral antigen expression in the tissues of the infected animals.

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“…Three of these polypeptides have been purified to homogeneity, namely, p24 (10), p15 (16), and gp6O (7), and their virus-coded nature is suggested by the fact that infected animals synthesize antibodies against them, a characteristic now widely used in the treatment of the disease (5).…”

mentioning

confidence: 99%

Translation of bovine leukemia virus virion RNAs in heterologous protein-synthesizing systems

Ghysdael¹,

Kettmann²,

Burny³

1979

J Virol

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Bovine leukemia virus 60 to 70S RNA was heat denatured, the polyadenylic acid-containing species were separated by velocity sedimentation, and several size classes were translated in a micrococcal nuclease-treated cell-free system from rabbit reticulocytes. The major RNA species sedimented at 38S and migrated as a single component of molecular weight 2.95 x 10' when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The predominant polypeptides of the in vitro translation of bovine leukemia virus 38S RNA were products with molecular weights of 70,000 and 45,000; minor components with molecular weights of 145,000 and 18,000 were also observed. Two lines of evidence indicate that the 70,000and 45,000-molecular weight polypeptides represent translation products of the gag gene of the bovine leukemia virus genome (Pr709"t' and Pr45,"('). First, they are specifically precipitated by a monospecific antiserum to the major internal protein, p24, and second, they are synthesized and correctly processed into virion proteins p24, p15, and plO in Xenopus laevis oocytes microinjected with bovine leukemia virus 38S RNA. The 145,000-molecular weight polypeptide was immunoprecipitated by the anti-p24 serum and not by an antiserum to the major envelope glycoprotein, gp6O. It contained all the tryptic peptides of Pr7g"t'(l and additional peptides unique to it, and thus represents an elongation product of Pr7e"'g' in an adjacent gene, presumably the pol gene. The 18,000-molecular weight product was antigenically unrelated to p24 and gp6O and shared no peptides in common with Pr701'', Pr451"', or the 145,000-molecular weight polypeptide. It was maximally synthesized on a polyadenylic acid-containing virion 16 to 18S RNA, and we present evidence that this RNA is a 3' endderived subgenomic fragment of the bovine leukemia virus genome rather than a contaminating cellular RNA.

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Isolation of a p15 polypeptide from bovine leukemia virus and detection of specific antibodies in leukemic cattle

Cited by 13 publications

References 16 publications

Spontaneous immune response of bovine leukemia‐virusinfected cattle against five different viral proteins

Spontaneous immune response of bovine leukemia‐virusinfected cattle against five different viral proteins

Bovine leukemia virus‐specific antibodies in french cattle. III. Prevalence of the BLV‐gp 51 radioimmunoassay for the detection of BLV‐infected animals

Translation of bovine leukemia virus virion RNAs in heterologous protein-synthesizing systems

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