Isolation of a Novel Lytic Bacteriophage against a Nosocomial Methicillin-Resistant Staphylococcus aureus Belonging to ST45

Pertics, Botond Zsombor; Szénásy, Dalma; Dunai, D.; Born, Yannick; Fieseler, Lars; Kovács, Tamás; Schneider, G.

doi:10.1155/2020/5463801

Cited by 7 publications

(5 citation statements)

References 60 publications

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“…This study was approved by Faculty of Pharmacy Ain Shams University Ethics Committee Number, ACUC-FP-ASU RHDIRB2020110301 REC #68. The sources of the respective specimens were chosen based on the possibility of incorporating S. aureus and hence bacteriophages specific against it ( Table 1 ) ( Clokie and Kropinski, 2009 ; Wommack et al., 2009 ; Hallajzadeh et al., 2019 ; Hyman, 2019 ; Moodley et al., 2019 ; Pertics et al., 2020 ). All samples were stored at 4°C until time of processing.…”

Section: Methodsmentioning

confidence: 99%

An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization

Abd-Allah

El-Housseiny

Alshahrani

et al. 2022

Front. Cell. Infect. Microbiol.

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Accumulating evidence has denoted the danger of resistance in tenacious organisms like methicillin-resistant Staphylococcus aureus (MRSA). MRSA, a supple bacterium that adopts a variety of antibiotic resistance mechanisms, is the cause of multiple life-threatening conditions. Approaching a post-antibiotic era, bacteria-specific natural predators, bacteriophages, are now given the chance to prove eligible for joining the antibacterial weaponry. Considering the foregoing, this study aimed at isolating bacteriophages with promising anti-MRSA lytic activity, followed by characterization and optimization of the production of the bacteriophage with the broadest host range. Five phages were isolated from different environmental sources including the rinse of raw chicken egg, raw milk, and, remarkably, the raw meat rinses of chicken and fish. Examined for lytic activity against a set of 23 MRSA isolates collected from various clinical specimens, all five phages showed relatively broad host ranges with the bacteriophage originally isolated from raw fish rinse showing lytic activity against all the isolates tested. This phage is suggested to be a member of Siphoviridae family, order Caudovirales, as revealed by electron microscopy. It also exhibited good thermal stability and viability at different pH grades. Moreover, it showed reasonable stability against UV light and all viricidal organic solvents tested. Optimization using D-optimal design by response surface methodology was carried out to enhance the phage yield. The optimum conditions suggested by the generated model were a pH value of 7, a carbon source of 0.5% w/v sucrose, and a nitrogen source of 0.1% w/v peptone, at a temperature of 28°C and a bacterial inoculum size of 107 CFU/ml, resulting in a 2 log-fold increase in the produced bacteriophage titer. Overall, the above findings indicate the lytic ability inflicted by this virus on MRSA. Apparently, its stability under some of the extreme conditions tested implies its potential to be a candidate for pharmaceutical formulation as an anti-MRSA therapeutic tool. We hope that bacteriophages could tip the balance in favor of the human front in their battle against multidrug-resistant pathogens.

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Section: Methodsmentioning

confidence: 99%

An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization

Abd-Allah

El-Housseiny

Alshahrani

et al. 2022

Front. Cell. Infect. Microbiol.

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“…The lysin (ORF26) component consisted of an N-terminal CHAP endopeptidase domain (pfam 05,257, amino acids 27–115) and an N-acetylmuramoyl-L-alanine-amidase domain (MurNAc-LAA, cd02696, amino acids 173–355), which was the same as the domain components of the lysin in phage Ph28 (Muharram et al 2020 ). In addition, the lysin component of BM31 contained a SH3 peptidoglycan-binding domain (amino acids 377–438) in the C-terminus, which confirms that modularity is prevalent in staphylococcal lysins (Oliveira et al 2013 ; Pertics et al 2020 ). The holin (ORF25) component belongs to the SPP1 family (PF04688), which corresponds to 90.59% similarity to an uncultured Caudovirales phage from South Africa (van Zyl et al 2018 ).…”

Section: Resultsmentioning

confidence: 61%

Characteristics of a novel temperate bacteriophage against Staphylococcus arlettae (vB_SarS_BM31)

et al. 2022

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Background Staphylococcus arlettae is a rarely reported coagulase-negative staphylococcus (CoNS) isolated from infected humans and livestock. Observing phage-bacteria interaction could improve the understanding of bacterial pathogenetic mechanisms, providing foundational evidence for phage therapy or phage detection. Herein, we aimed to characterise and annotate a novel bacteriophage, vB_SarS_BM31 (BM31), specific to S. arlettae . This bacteriophage was isolated from a milk sample associated with bovine mastitis and collected in the Sichuan Province, China. Results The BM31 genome comprised a linear double-stranded DNA of 42,271 base pair in length with a G + C content of 34.59%. A total of 65 open reading frames (ORFs) were assembled from phage DNA, of which 29 were functionally annotated. These functional genes were divided into four modules: the structural, DNA packing and replication, lysis, and lysogeny modules. Holin (ORF25), lysin (ORF26), and integrase (ORF28) were located closely in the entire BM31 genome and were important for lyse or lysogeny cycle of BM31. The phage was identified as a temperate phage according to whole genome analysis and life cycle assay, with basic biological characteristics such as small burst size, short latency period, and narrow host range, consistent with the characteristics of the family Siphoviridae, subcluster B14 of the Staphylococcus bacteriophage. Conclusions The present isolation and characterisation of BM31 contributes to the Staphylococcus bacteriophage database and provides a theoretical foundation for its potential applications. To the best of our knowledge, BM31 is the only shared and completely reported phage against S. arlettae in the entire public database. Supplementary Information The online version contains supplementary material available at 10.1007/s10123-022-00292-3.

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“…In addition, one prolate phage with a tail which was longer than those of the icosahedral phages was detected in the preparation of Dubai-30 (Figure 3E). Its morphology resembled prolate-headed Triavirus specimens (i.e., the genus to which phiSa2wa_st78 was previously assigned [51,54]), allowing the assumption to be made that this was the PVL phage.…”

Section: Transmission Electron Microscopy Of Phagesmentioning

confidence: 98%

Characterisation of PVL-Positive Staphylococcus argenteus from the United Arab Emirates

Monecke,

Burgold-Voigt,

Braun

et al. 2024

Antibiotics

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Staphylococcus argenteus is a recently described staphylococcal species that is related to Staphylococcus aureus but lacks the staphyloxanthin operon. It is able to acquire both resistance markers such as the SCCmec elements and mobile genetic elements carrying virulence-associated genes from S. aureus. This includes those encoding the Panton–Valentine leukocidin (PVL), which is associated mainly with severe and/or recurrent staphylococcal skin and soft tissue infections. Here, we describe the genome sequences of two PVL-positive, mecA-negative S. argenteus sequence type (ST) 2250 isolates from the United Arab Emirates in detail. The isolates were found in a dental clinic in the United Arab Emirates (UAE). Both were sequenced using Oxford Nanopore Technology (ONT). This demonstrated the presence of temperate bacteriophages in the staphylococcal genomes, including a PVL prophage. It was essentially identical to the published sequence of phiSa2wa_st78 (GenBank NC_055048), a PVL phage from an Australian S. aureus clonal complex (CC) 88 isolate. Besides the PVL prophage, one isolate carried another prophage and the second isolate carried two additional prophages, whereby the region between these two prophages was inverted. This “flipped” region comprised about 1,083,000 bp, or more than a third of the strain’s genome, and it included the PVL prophage. Prophages were induced by Mitomycin C treatment and subjected to transmission electron microscopy (TEM). This yielded, in accordance to the sequencing results, one or, respectively, two distinct populations of icosahedral phages. It also showed prolate phages which presumptively might be identified as the PVL phage. This observation highlights the significance bacteriophages have as agents of horizontal gene transfer as well as the need for monitoring emerging staphylococcal strains, especially in cosmopolitan settings such as the UAE.

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Isolation of a Novel Lytic Bacteriophage against a Nosocomial Methicillin-Resistant Staphylococcus aureus Belonging to ST45

Cited by 7 publications

References 60 publications

An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization

An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization

Characteristics of a novel temperate bacteriophage against Staphylococcus arlettae (vB_SarS_BM31)

Characterisation of PVL-Positive Staphylococcus argenteus from the United Arab Emirates

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