Isolation of a novel family of genes related to 2-oxoglutarate-dependent dioxygenases from soybean and analysis of their expression during root nodule senescence

The Legume Root Nodule: From Symbiotic Nitrogen Fixation to Senescence

“…In order to isolate genes up-or down-regulated during nodule senescence, several genetic analyses including cDNA libraries and differential screening, mRNA differential display or cDNA-AFLP, have been performed in soybean (Alesandrini et al, 2003;Webb et al, 2008), and M. truncatula (Fedorova et al, 2002;Van de Velde et al, 2006). Using a mixture of effective nodules from 7 week-old plants, the first database specific to M. truncatula nodule senescence was obtained by isolating 140 000 Expressed Sequence Tags which are available in the J. Craig Venter Institute (http://www.jcvi.org/cms/research/groups/plant-genomics/resources/).…”

“…Amongst these genes, proteins associated to proteasome function and vesicular trafficking are not induced during dark stress suggesting partial different regulatory processes between the two types of senescence. In soybean, a screen for genes involved in root nodule senescence has led to the isolation of the senescence-associated nodulin 1 (SAN1) multigene family showing a high homology with plant 2-oxoglutarate-dependent dioxygenases and including two functional genes SAN1A and SAN1B and a pseudogene SAN1C (Webb et al, 2008). Analyses of the steady-state mRNA levels of SAN1A and SAN1B during developmental senescence showed no significant differences for both genes.…”

Exogenous hydrogen sulphide alleviates nodule senescence in Glycine max-Sinorhizobium fredii symbiotic system

“…7H). Webb et al (2008) reported that they isolated three senescence-associated nodulin1 (SAN1) genes and during nodule senescence the expression levels of SAN1 was induced. Our results was consisted with the report of Webb et al (2008), found that at late stage of symbiosis the expression abundances of GmSAN1 was induced, but H 2 S inhibited this gene highly expression.…”

“…Webb et al (2008) reported that they isolated three senescence-associated nodulin1 (SAN1) genes and during nodule senescence the expression levels of SAN1 was induced. Our results was consisted with the report of Webb et al (2008), found that at late stage of symbiosis the expression abundances of GmSAN1 was induced, but H 2 S inhibited this gene highly expression. It is known that antioxidant plays an important role in plant growth and development, and antioxidant enzyme activities are altered during nodules senescence (Puppo et al 2005).…”

Biologically Active Constituents of Soybean

“…SAN1A is downregulated whereas SAN1B is upregulated during senescence induced by treatment with fixed nitrogen or darkness. The expression of the SAN1genes is not found only in nodules, signifying a more general role in plant metabolism [52].…”