Isolation of a New Virus, HBLV, in Patients with Lymphoproliferative Disorders

Abstract

A novel human B-lymphotropic virus (HBLV) was isolated from the peripheral blood leukocytes of six individuals: two HTLV-III seropositive patients from the United States (one with AIDS-related lymphoma and one with dermatopathic lymphadenopathy), three HTLV-III seronegative patients from the United States (one with angioimmunoblastic lymphadenopathy, one with cutaneous T-cell lymphoma, and one with immunoblastic lymphoma), and one HTLV-III seronegative patient with acute lymphocytic leukemia from Jamaica. All six isolates were closely related by antigenic analysis, and sera from all six virus-positive patients reacted immunologically with each virus isolate. In contrast, only four sera from 220 randomly selected healthy donors and none from 12 AIDS patients without associated lymphoma were seropositive. The virus selectively infected freshly isolated human B cells and converted them into large, refractile mono- or binucleated cells with nuclear and cytoplasmic inclusion bodies. HBLV is morphologically similar to viruses of the herpesvirus family but is readily distinguishable from the known human and nonhuman primate herpesviruses by host range, in vitro biological effects, and antigenic features.

Production of IgM antibody to HHV6 in reactivation and primary infection

“…Two other research groups also described the isolation of similar viruses from patients with AIDS or other lymphoproliferative disorders [2,3]. These viruses have double-stranded DNA with a genome of approximately 110 kb pairs [2].…”

“…Two other research groups also described the isolation of similar viruses from patients with AIDS or other lymphoproliferative disorders [2,3]. These viruses have double-stranded DNA with a genome of approximately 110 kb pairs [2]. Endonuclease restriction mapping of the genome and serological studies suggested that these isolates comprised a newly recognized human herpesvirus which has now been designated tentatively as human herpesvirus type 6 (HHV6).…”

Successful treatment of severe aplastic anemia associated with human parvovirus B19 and Epstein‐Barr virus in a healthy subject with allo‐BMT

“…Cellular suppression of normal CFU-GM-derived colony formation by co-culture with the patient's bone marrow mononuclear cells has been demonstrated in 17-year-old female with EBV-related aplastic anemia [13]. In our case, the response to treatment with prednisone was poor, which may be explained by (a) his bone marrow suppression might have resulted from a combination effect of direct viral infection and immune-mediated suppression/destruction, (b) the presence of other viruses as in reported other cases [17,18], and (c) an insufficient immunosuppression.…”

Understanding the association between chromosomally integrated human herpesvirus 6 and HIV disease: a cross-sectional study

“…The virus infects lymphocytes with a predominant tropism for CD4+ T cells 1 . In individuals with ciHHV-6, the entire viral genome is present in every nucleated cell of the body and transmitted in a Mendelian fashion 2, 3 .…”