Isolation of a new Thermoanaerobacterium thermosaccharolyticum strain (FH1) producing a thermostable dextranase.

Hirth, Frank; Daniel, Rolf; Gottschalk, Gerhard

doi:10.2323/jgam.47.187

Cited by 31 publications

(13 citation statements)

References 18 publications

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“…Strain KKU19 showed an ability to grow on a wide variety of simple and complex carbohydrates (Table 1), which is the characterization of T. thermosaccharolyticum. The bacterium could utilize almost all of the ranges of substrate except rhamnose and dextran, similar to T. thermosaccharolyticum strain DSM571 [36], while except peptone was similar to T. thermosaccharolyticum strain FH1 [37]. The ability of strain KKU19 to utilize the substrate was identical to strain PSU-2 [6].…”

Section: Characterization Of the Strain Kku19mentioning

confidence: 86%

Biohydrogen production from xylose by Thermoanaerobacterium thermosaccharolyticum KKU19 isolated from hot spring sediment

Khamtib

Reungsang

2012

International Journal of Hydrogen Energy

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Section: Characterization Of the Strain Kku19mentioning

confidence: 86%

Biohydrogen production from xylose by Thermoanaerobacterium thermosaccharolyticum KKU19 isolated from hot spring sediment

Khamtib

Reungsang

2012

International Journal of Hydrogen Energy

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“…Thus in the year 2001 German researchers published the isolation of a strain of the thermophylic, Gram positive and strictly anaerobic dextranase producing bacteria Thermoanaerobacterium thermosaccharolyticum (Hoster et al, 2001). The enzyme showed maximum activity at pH 5.5 and between 65 and 70 ºC.…”

Section: Chronology Of the Isolation Of Dextranase Producing Microorgmentioning

confidence: 99%

“…Dextranlyticum (Sugiura et al, 1974) 7.0 -7.5 NR Streptococcus mutans (Ellis and Miller, 1977) 5.5 37 ºC Streptomyces anulatus (two enzymes) (Decker et al, 2003) 7.0 40 and 50 ºC Flavobacterium sp. M-73 (Kobayashi et al, 1983) 7.0 35 ºC Thermoanaerobacter wiegelii (Wynter et al, 1996) 5.5 70 ºC Strain of Thermoanaerobacter (Wynter et al, 1997) 4.5 -5.5 80 ºC Thermoanaerobacterium thermosaccharolyticum (Hoster et al, 2001) 5.5 65 -70 ºC…”

Section: Dextranase Enzymatic Preparations For the Sugar Industrymentioning

confidence: 99%

Dextranase in sugar industry: A review

Jiménez

2009

Sugar Tech

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In sugar production, dextrans are undesirable compounds synthesized by contaminant microorganisms from sucrose, increasing the viscosity of the flow and reducing industrial recovery, bringing about significant losses. The use of the dextranase enzyme is the most efficient method for hydrolyzing the dextrans at sugar mills. Some bacterial strains, filamentous fungi and a small number of yeasts have been shown to produce dextranase. The fungal dextranases showed the highest reaction rate at low Brix, with pH and temperature close to 5.0 and 50 ºC, respectively, that is, conditions existing in juice extraction. Some of these dextranases formulated in enzymatic preparations have been efficiently used for hydrolyzing dextrans in sugar mill juices. In more advanced stage of the process, where the dextrans have already caused losses, the conditions of temperature and Brix are high. However, although the volumes are smaller, the treatment with these enzymes in syrup showed the need to increase the dose, equaling dextranase consumption. Some thermo tolerant bacterial dextranases identified up to now showed a much reduced specific activity that makes their industrial use unfeasible. The fungal dextranases from Chaetomium sp. have shown the best results on dextrans treatment both in juices and syrups. Any attempt to obtain a new natural or recombinant dextranase enzyme, must be comparable with the Chaetomium enzyme.

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“…The chromatography of products from dextran T-500 with crude enzyme suggested a random endo-type hydrolysis resulting in liberation of long-chain oligomers together with glucose and isomaltose units ( Table 1). The production and characteristics of thermostable dextranases have been reported (69,227,228). Among the isolates, Thermoanaerobacter sp.…”

Section: Vol 69 2005 Microbial Dextran-hydrolyzing Enzymes 311mentioning

confidence: 99%

“…The enzymatic method seemed to have potential for replacing the acid hydrolysis for clinical dextran production and such VOL. 69,2005 MICROBIAL DEXTRAN-HYDROLYZING ENZYMES 319…”

Section: Clinical Applications Of Dextran and Dextranasesmentioning

confidence: 99%

Microbial Dextran-Hydrolyzing Enzymes: Fundamentals and Applications

Khalikova

Susi

Korpela

2005

Microbiol Mol Biol Rev

227

152

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Dextran is a chemically and physically complex polymer, breakdown of which is carried out by a variety of endo- and exodextranases. Enzymes in many groups can be classified as dextranases according to function: such enzymes include dextranhydrolases, glucodextranases, exoisomaltohydrolases, exoisomaltotriohydrases, and branched-dextran exo-1,2-α-glucosidases. Cycloisomalto-oligosaccharide glucanotransferase does not formally belong to the dextranases even though its side reaction produces hydrolyzed dextrans. A new classification system for glycosylhydrolases and glycosyltransferases, which is based on amino acid sequence similarities, divides the dextranases into five families. However, this classification is still incomplete since sequence information is missing for many of the enzymes that have been biochemically characterized as dextranases. Dextran-degrading enzymes have been isolated from a wide range of microorganisms. The major characteristics of these enzymes, the methods for analyzing their activities and biological roles, analysis of primary sequence data, and three-dimensional structures of dextranases have been dealt with in this review. Dextranases are promising for future use in various scientific and biotechnological applications

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Isolation of a new Thermoanaerobacterium thermosaccharolyticum strain (FH1) producing a thermostable dextranase.

Cited by 31 publications

References 18 publications

Biohydrogen production from xylose by Thermoanaerobacterium thermosaccharolyticum KKU19 isolated from hot spring sediment

Biohydrogen production from xylose by Thermoanaerobacterium thermosaccharolyticum KKU19 isolated from hot spring sediment

Dextranase in sugar industry: A review

Microbial Dextran-Hydrolyzing Enzymes: Fundamentals and Applications

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