Isolation of a mouse brain cDNA expressed in developing neuroblasts and mature neurons

Boutou, Effrossyni; Matsas, Rebecca; Mamalaki, Avgi

doi:10.1016/s0169-328x(00)00281-3

Cited by 20 publications

(14 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Figure 1B, mRNAs for schwannomin-interacting protein 1 (SCHIP-1), 26 mc7 (dystrophin-interacting protein), 27 thymosin ␤4 (G-actin sequestering protein), 28 and L-arginine:glycine (Arg/Gly) amidinotransferase 29 are more abundantly expressed in mdx-sm cells than in B10-sm cells, whereas the mRNA levels of GARG16 (glucocorticoid-attenuated response gene), 30 selenoprotein P (anti--oxidant protein), 31 and EST-MNCb1423 (accession number, XM 149185; designated as RAMP in this study) are lower in mdx-sm cells. In addition, eight other genes including myogenic transcription factor MEFC 32 were found to be up-regulated in mdx-sm cells while four other genes were down-regulated (data not shown, Table 1).…”

Section: Identification Of Differentially Expressed Genes In Mdx-sm Cmentioning

confidence: 98%

Cloning of cDNA Encoding a Regeneration-Associated Muscle Protease Whose Expression Is Attenuated in Cell Lines Derived from Duchenne Muscular Dystrophy Patients

Nakayama¹,

Nara²,

Kawakita³

et al. 2004

The American Journal of Pathology

View full text Add to dashboard Cite

In the dystrophin-mutant mdx mouse, an animal model for Duchenne muscular dystrophy (DMD), damaged skeletal muscles are efficiently regenerated and thus the animals thrive. The phenotypic differences between DMD patients and the mdx mice suggest the existence of factors that modulate the muscle wasting in the mdx mice. To identify these factors, we searched for mRNAs affected by the mdx mutation by using cDNA microarrays with newly established skeletal muscle cell lines from mdx and normal mice. We found that in the mdx muscle cell line, 12 genes, including L-arginine:glycine amidinotransferase and thymosin ␤4, are up-regulated, whereas 7 genes, including selenoprotein P and a novel regeneration-associated muscle protease (RAMP), are down-regulated. Northern blot analysis and in situ hybridization revealed that RAMP mRNA is predominantly expressed in normal skeletal muscle and brain, and its production is enhanced in the regenerating area of injured skeletal muscle in mice. RAMP expression was much lower in individual muscle cell lines derived from biopsies of six DMD patients compared to a normal muscle cell line. These results suggest that RAMP may play a role in the regeneration of skeletal muscle and that its down-regulation could be involved in the progression of DMD in humans.

show abstract

Section: Identification Of Differentially Expressed Genes In Mdx-sm Cmentioning

confidence: 98%

Cloning of cDNA Encoding a Regeneration-Associated Muscle Protease Whose Expression Is Attenuated in Cell Lines Derived from Duchenne Muscular Dystrophy Patients

Nakayama¹,

Nara²,

Kawakita³

et al. 2004

The American Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…TRIM‐containing protein 44 (TRIM44), a member of the tripartite motif (TRIM) protein family, contains B‐box, coiled‐coil domains and a zinc‐finger domain that is also found in ubiquitin hydrolases . TRIM44, a protein that may function as a “USP‐like TRIM”, was originally isolated from a mouse brain cDNA library . Accumulating data suggest that TRIM44 plays stimulatory and cancerogenic roles in multiple cancers.…”

Section: Introductionmentioning

confidence: 99%

“…mouse brain cDNA library. 8,9 Accumulating data suggest that TRIM44 plays stimulatory and cancerogenic roles in multiple cancers. TRIM44 overexpression facilitates cell proliferation, migration, and invasion in gastric cancer, 10 hepatocellular carcinoma, 11 testicular germ cell tumors, 12 and non-small cell lung cancer (NSCLC).…”

mentioning

confidence: 99%

TRIM44 promotes human esophageal cancer progression via the AKT/mTOR pathway

Xiong

Chun

et al. 2018

Cancer Science

View full text Add to dashboard Cite

Aberrant expression of TRIM‐containing protein 44 (TRIM44) acts as a promoter in multiple cancers. Here, we investigated the biological functions and clinical significance of TRIM44 in human esophageal cancer (HEC). TRIM44 expression was significantly higher in HEC tissues than corresponding normal tissues at both the mRNA (2.42 ± 0.52 vs 0.99 ± 0.25) and protein (1.01 ± 0.27 vs 0.30 ± 0.13) levels. Patients with high TRIM44 expression showed poor differentiation (P = 1.39 × 10−5), advanced TNM stage (P = 3.87 × 10−4) and, most importantly, significantly poorer prognosis (P = 2.80 × 10−5). TRIM44 played a crucial role in epithelial mesenchymal transition (EMT). A significant correlation was observed between TRIM44 and Ki67 expression. We demonstrated that TRIM44 markedly enhanced HEC cell proliferation, migration, and invasion. Additionally, TRIM44 was involved in the AKT/mTOR signaling pathway and its downstream targets, such as STAT3 phosphorylation. Thus, elevated TRIM44 expression promotes HEC development by EMT via the AKT/mTOR pathway, and TRIM44 may be a novel prognostic indicator for HEC patients after curative resection.

show abstract

“…TRIM44 was originally isolated from a mouse brain cDNA library . TRIM44 is considered to be associated with several malignancies, such as lung cancer, gastric cancer, esophageal cancer, and head/neck squamous cell carcinoma .…”

mentioning

confidence: 99%

A novel prognostic factor TRIM44 promotes cell proliferation and migration, and inhibits apoptosis in testicular germ cell tumor

et al. 2016

View full text Add to dashboard Cite

Tripartite motif 44 (TRIM44) is one of the TRIM family proteins that are involved in ubiquitination and degradation of target proteins by modulating E3 ubiquitin ligases. TRIM44 overexpression has been observed in various cancers. However, its association with testicular germ cell tumor (TGCT) is unknown. We aimed to investigate the clinical significance of TRIM44 and its function in TGCT. High expression of TRIM44 was significantly associated with α feto‐protein levels, clinical stage, nonseminomatous germ cell tumor (NSGCT), and cancer‐specific survival (P = 0.0009, P = 0.0035, P = 0.0004, and P = 0.0140, respectively). Multivariate analysis showed that positive TRIM44 IR was an independent predictor of cancer‐specific mortality (P = 0.046). Gain‐of‐function study revealed that overexpression of TRIM44 promoted cell proliferation and migration of NTERA2 and NEC8 cells. Knockdown of TRIM44 using siRNA promoted apoptosis and repressed cell proliferation and migration in these cells. Microarray analysis of NTERA2 cells revealed that tumor suppressor genes such as CADM1,CDK19, and PRKACB were upregulated in TRIM44‐knockdown cells compared to control cells. In contrast, oncogenic genes including C3AR1,ST3GAL5, and NT5E were downregulated in those cells. These results suggest that high expression of TRIM44 is associated with poor prognosis and that TRIM44 plays significant role in cell proliferation, migration, and anti‐apoptosis in TGCT.

show abstract

Isolation of a mouse brain cDNA expressed in developing neuroblasts and mature neurons

Cited by 20 publications

References 67 publications

Cloning of cDNA Encoding a Regeneration-Associated Muscle Protease Whose Expression Is Attenuated in Cell Lines Derived from Duchenne Muscular Dystrophy Patients

Cloning of cDNA Encoding a Regeneration-Associated Muscle Protease Whose Expression Is Attenuated in Cell Lines Derived from Duchenne Muscular Dystrophy Patients

TRIM44 promotes human esophageal cancer progression via the AKT/mTOR pathway

A novel prognostic factor TRIM44 promotes cell proliferation and migration, and inhibits apoptosis in testicular germ cell tumor

Contact Info

Product

Resources

About