Isolation of a methyl-reducing methanogen outside the Euryarchaeota

Cheng, Lei; Wu, Ke‐Jia; Zhou, Lei; Tahon, Guillaume; Liu, Laiyan; Li, Jiang; Zhang, Jianchao; Zheng, Fengfeng; Deng, Chengpeng; Han, Wenfeng; Bai, Liping; Fu, Lin; Dong, Xiuzhu; Zhang, Chuanlun; Ettema, Thijs J. G.; Sousa, Diana Z.

doi:10.21203/rs.3.rs-2501667/v1

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…The rumen is mainly inhabited by hydrogenotrophic methanogens [37]. The second class, acetoclastic methanogens which are most commonly present in freshwater sediments and anaerobic digestors, but use alcohols such as ethanol or 2-propanol as electron donors to produce CH 4 [38][39][40][41][42]. The methylotrophic class which is predominant in freshwater and wetland soils [43][44][45] relies on methyl groups such as methanol and methylamines to produce CH 4 , and encompasses the order Methanococcales and Methanosarcinales.…”

Section: Brief Overview Of Methanogensmentioning

confidence: 99%

Holistic View and Novel Perspective on Ruminal and Extra-Gastrointestinal Methanogens in Cattle

Aryee,

Luecke,

Dahlen

et al. 2023

Microorganisms

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Despite the extensive research conducted on ruminal methanogens and anti-methanogenic intervention strategies over the last 50 years, most of the currently researched enteric methane (CH4) abatement approaches have shown limited efficacy. This is largely because of the complex nature of animal production and the ruminal environment, host genetic variability of CH4 production, and an incomplete understanding of the role of the ruminal microbiome in enteric CH4 emissions. Recent sequencing-based studies suggest the presence of methanogenic archaea in extra-gastrointestinal tract tissues, including respiratory and reproductive tracts of cattle. While these sequencing data require further verification via culture-dependent methods, the consistent identification of methanogens with relatively greater frequency in the airway and urogenital tract of cattle, as well as increasing appreciation of the microbiome–gut–organ axis together highlight the potential interactions between ruminal and extra-gastrointestinal methanogenic communities. Thus, a traditional singular focus on ruminal methanogens may not be sufficient, and a holistic approach which takes into consideration of the transfer of methanogens between ruminal, extra-gastrointestinal, and environmental microbial communities is of necessity to develop more efficient and long-term ruminal CH4 mitigation strategies. In the present review, we provide a holistic survey of the methanogenic archaea present in different anatomical sites of cattle and discuss potential seeding sources of the ruminal methanogens.

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Section: Brief Overview Of Methanogensmentioning

confidence: 99%

Holistic View and Novel Perspective on Ruminal and Extra-Gastrointestinal Methanogens in Cattle

Aryee,

Luecke,

Dahlen

et al. 2023

Microorganisms

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“…(Joshi et al, 2018;Kumar et al, 2018). Methanogens are currently documented under four phyla, namely, Euryarchaeota, Thermoproteota, Bathyarchaeota, and Verstraetearchaeota (Berghuis et al, 2019;Evans et al, 2015;Vanwonterghem et al, 2016;Zhou et al, 2018), although the cultured isolates till date are only from phylum Euroyarchaeota, Verstraetearchaeota and Thermoproteota (Cheng et al, 2023;Hatzenpichler, 2023). Phylogenetically, methanogens are divided into ve families, comprising 33 genera and 153 species and can survive over a broad temperature range of 0 ℃ to 110 ℃ and pH of 4.1 to 10 (Franzmann et al, 1997;Michał et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetically and physiologically diverse methanogenic archaea inhabit the Indian hot spring environments

Deore,

Dhakephalkar,

Dagar

2023

Arch Microbiol

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Mesophilic and thermophilic methanogens belonging to the hydrogenotrophic, methylotrophic, and acetotrophic groups were isolated from Indian hot spring environments using BY and BCYT growth media. Following initial Hinf I based PCR-RFLP screening, 70 methanogens were sequenced to ascertain their identity. These methanogens were phylogenetically and physiologically diverse and represented different taxa distributed across three physiological groups, i.e. hydrogenotrophs (53), methylotrophs (14) and acetotrophs (3). Overall, methanogens representing three families, ve genera, and ten species, including two putative novel species, were recognized. The highest number and diversity of methanogens was observed at 40℃, dominated by Methanobacterium (10; 3 species), Methanosarcina (9; 3 species), Methanothermobacter (7; 2 species), Methanomethylovorans (5; 1 species) and Methanoculleus (3; 1 species). Both putative novel methanogen species were isolated at 40℃ and belonged to the genera Methanosarcina and Methanobacterium. At 55℃, limited diversity was observed, and resulted in the isolation of only two genera of methanogens, i.e., Methanothermobacter (28; 2 species) and Methanosarcina (4; 1 species). At 70℃, only members of the genus Methanothermobacter (5; 2 species) were isolated, whereas no methanogen could be cultured at 85℃. Ours is the rst study that documents the extensive range of cultivable methanogenic archaea inhabiting hot springs across various geothermal provinces of India.

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Methylotrophic methanogenesis in the Archaeoglobi revealed by cultivation ofCa.Methanoglobus hypatiae from a Yellowstone hot spring

Lynes,

Jay,

Kohtz

et al. 2023

Preprint

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Over the past decade, environmental metagenomics and PCR-based marker gene surveys have revealed that several lineages beyond just a few well-established groups within the Euryarchaeota superphylum harbor the genetic potential for methanogenesis. One of these groups are the Archaeoglobi, a class of obligately thermophilic Euryarchaeotes that have long been considered to live a non-methanogenic lifestyle. Here, we enrichedCandidatusMethanoglobus hypatiae, a methanogen affiliated with the family Archaeoglobaceae, from a hot spring in Yellowstone National Park. The enrichment is sediment-free, grows at 64-70 °C and a pH of 7.8, and produces methane from mono-, di-, and tri-methylamine.Ca.M. hypatiae is represented by a 1.62 Mb metagenome-assembled genome with an estimated completeness of 100% and accounts for up to 67% of cells in the culture according to fluorescencein situhybridization. Via genome-resolved metatranscriptomics and stable isotope tracing, we demonstrate thatCa.M. hypatiae expresses methylotrophic methanogenesis and energy-conserving pathways for reducing monomethylamine to methane. The detection of Archaeoglobi populations related toCa.M. hypatiae in 36 geochemically diverse geothermal sites within Yellowstone National Park (pH 2.61-9.35; 18.4 to 93.8 °C), as revealed through the examination of previously published gene amplicon datasets, implies a previously underestimated contribution to anaerobic carbon cycling in extreme ecosystems.Significance statementThe majority of global methane emissions are attributed to the activity of methane-producing anaerobic archaea, the methanogens. Over the last decade, environmental DNA sequencing demonstrated that culture collections do not adequately represent the true taxonomic and metabolic diversity of methanogens present in nature. One group of archaea postulated to contribute to methane production in high temperature marine and terrestrial environments are the Archaeoglobi, a group of obligate thermophilic archaea within the Euryarchaeota superphylum. Here, we report the cultivation and physiological characterization of a methanogenic member of the Archaeoglobi,Ca.Methanoglobus hypatiae, from a hot spring in Yellowstone National Park. Via a combination of growth experiments, stable isotope tracing, metatranscriptomics, microscopy analyses, and re-examination of gene amplicon surveys, our study provides direct experimental evidence of methanogenesis in the Archaeoglobi and shows that closely related archaea are widely distributed in Yellowstone’s geothermal features.

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Isolation of a methyl-reducing methanogen outside the Euryarchaeota

Cited by 5 publications

References 28 publications

Holistic View and Novel Perspective on Ruminal and Extra-Gastrointestinal Methanogens in Cattle

Holistic View and Novel Perspective on Ruminal and Extra-Gastrointestinal Methanogens in Cattle

Phylogenetically and physiologically diverse methanogenic archaea inhabit the Indian hot spring environments

Methylotrophic methanogenesis in the Archaeoglobi revealed by cultivation ofCa.Methanoglobus hypatiae from a Yellowstone hot spring

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