Isolation of a membrane associated iron chelator from Pseudomonas aeruginosa

Royt, Paulette W.

doi:10.1016/0005-2736(88)90096-x

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…The reaction mixture was extracted with 75 ml of methylene chloride followed by two subsequent extractions of 75 ml of methylene chloride. The combined methylene chloride layers were washed with three 100 ml portions of water and dried over MgSO 4 . The solution was suction filtered and evaporated in vacuo.…”

Section: Synthesis Of 4-hydroxy-2-nonylquinolinementioning

confidence: 99%

“…A study of iron transport and metabolism in Pseudomonas aeruginosa led to the isolation of an iron chelator associated with the cytoplasmic membrane of iron-rich cells (4). Known as the membrane associated iron chelator (MAIC), the compound is extracted from membranes with ethanol, and in earlier studies, isolated on thinlayer chromatograms.…”

Section: Introductionmentioning

confidence: 99%

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4-Hydroxy-2-nonylquinoline: A Novel Iron Chelator Isolated from a Bacterial Cell Membrane

Royt¹,

Honeychuck

Ravich³

et al. 2001

Bioorganic Chemistry

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Section: Synthesis Of 4-hydroxy-2-nonylquinolinementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

4-Hydroxy-2-nonylquinoline: A Novel Iron Chelator Isolated from a Bacterial Cell Membrane

Royt¹,

Honeychuck

Ravich³

et al. 2001

Bioorganic Chemistry

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“…Entrance pathways for these compounds remain unknown. Finally, in addition to these well-demonstrated iron transport systems, other hypothetical methods could involve, for example, membrane-associated iron chelators (Royt, 1988) or even less-defined, lowaffinity systems (Neilands et ai., 1987) to explain the ability of bacteria to grow well in an iron-rich environment without apparently producing any siderophores.…”

Section: Iron Uptake From Other Iron Sourcesmentioning

confidence: 99%

Iron Metabolism and Siderophores in Pseudomonas and Related Species

Meyer¹,

Stintzi²

1998

Pseudomonas

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“…Many microorganisms are also able to utilize the Fe3" complexes of siderophores which they have not synthesized (8). The importance of Fe is reflected by the number of mechanisms developed by microorganisms for its acquisition, which include a membrane-bound chelator (31), reduction of Fe chelates (10,19), and an unknown mechanism in Serratia marcescens (38) in addition to those already mentioned.…”

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confidence: 99%

Differential Siderophore Utilization and Iron Uptake by Soil and Rhizosphere Bacteria

Jurkevitch

Hadar

Chen

1992

Appl Environ Microbiol

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The differential availabilities of the hydroxamate siderophores ferrioxamine B (FOB) and ferrichrome (FC) and the pseudobactin siderophores St3, 7NSK2, and WCS 358 as sources of Fe for soil and rhizosphere bacteria were studied. About 20% of the total bacterial CFU from the rhizospheres of four plant species were able to use FOB as the sole Fe source in an Fe-deficient medium, while about 12, 10, 2, and >1 % were able to use FC and pseudobactins 7NSK2, St3, and WCS 358, respectively. Of the 165 colonies isolated from plates containing pseudobactins, 64 were able to use the pseudobactin on which they were isolated as the sole Fe source in pure culture. Cross-feeding tests showed that almost all of these 64 strains were also able to use at least one of the other siderophores studied (pseudobactin, FOB, or FC). Pseudomonas putida StS2, Pseudomonas maltophilia 7NM1, and Vibriofluvialis WS1, which were originally isolated on pseudobactins St3, 7NSK2, and WCS 358, respectively, were selected for their ability to grow with pseudobactin St3 as the sole Fe source. They incorporated 55Fe3+ mediated by pseudobactin St3 at various rates (71.5, 4, and 23 pmol/min/mg [dry weight] of cells, respectively). Similarly, P. putida St3 was shown to incorporate 55Fe3+ mediated by FOB and FC. We suggest that the ability of bacteria to utilize a large variety of siderophores confers an ecological advantage. Fe forms insoluble hydroxides at neutral and basic pHs (20). Microorganisms have therefore evolved efficient uptake mechanisms to obtain sufficient amounts of this essential element (26). Most aerobic and facultative aerobic bacteria possess a high-affinity Fe transport system in which siderophores are excreted and the consequent Fe complex is taken up via the cognate-specific receptor and a transport pathway * Corresponding author. their ability to utilize exogenous siderophores. Receptors for

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Isolation of a membrane associated iron chelator from Pseudomonas aeruginosa

Cited by 9 publications

References 19 publications

4-Hydroxy-2-nonylquinoline: A Novel Iron Chelator Isolated from a Bacterial Cell Membrane

4-Hydroxy-2-nonylquinoline: A Novel Iron Chelator Isolated from a Bacterial Cell Membrane

Iron Metabolism and Siderophores in Pseudomonas and Related Species

Differential Siderophore Utilization and Iron Uptake by Soil and Rhizosphere Bacteria

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