Isolation of a Human Intestinal Bacterium Capable of Daidzein and Genistein Conversion

Matthies, Anastasia; Blaut, Michaël; Braune, Annett

doi:10.1128/aem.01795-08

Cited by 139 publications

(127 citation statements)

References 40 publications

Supporting

Mentioning

123

Contrasting

Unclassified

Order By: Relevance

“…Lactococcus and Slackia are bacteria belonging to different phyla; the former is found mainly in fish and animal milk and is rarely isolated from human (7,24,27,28), whereas the latter inhabits the human intestines (11,19,22,26). At present, although the phyletic evolution of these enzyme genes is unknown, the interbacterium propagation of the genes and their physiological roles in the bacteria are of interest.…”

Section: Discussionmentioning

confidence: 99%

Identification of an Enzyme System for Daidzein-to-Equol Conversion in Slackia sp. Strain NATTS

Tsuji

Moriyama

Nomoto

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

An Escherichia coli library comprising 8,424 strains incorporating gene fragments of the equol-producing bacterium Slackia sp. strain NATTS was constructed and screened for E. coli strains having daidzein-and dihydrodaidzein (DHD)-metabolizing activity. We obtained 3 clones that functioned to convert daidzein to DHD and 2 clones that converted DHD to equol. We then sequenced the gene fragments inserted into plasmids contained by these 5 clones. All of the gene fragments were contiguous, encoding three open reading frames (ORF-1, -2, and -3). Analysis of E. coli strains containing an expression vector incorporating one of the orf-1, -2, or -3 genes revealed that (i) the protein encoded by orf-1 was involved in the conversion of cis/trans-tetrahydrodaidzein (cis/trans-THD) to equol, (ii) the protein encoded by orf-2 was involved in the conversion of DHD to cis/trans-THD, and (iii) the protein encoded by orf-3 was involved in the conversion of daidzein to DHD. ORF-1 had a primary amino acid structure similar to that of succinate dehydrogenase. ORF-2 was presumed to be an enzyme belonging to the short-chain dehydrogenase/reductase superfamily. ORF-3 was predicted to have 42% identity to the daidzein reductase of Lactococcus strain 20-92 and belonged to the NADH:flavin oxidoreductase family. These findings showed that the daidzein-to-equol conversion reaction in the Slackia sp. NATTS strain proceeds by the action of these three enzymes.S oybean isoflavones and their derivatives have been reported to prevent sex hormone-dependent diseases, such as prostate cancer, breast cancer, menopausal disorders, premenstrual syndrome, and osteoporosis (3,9,10,16,21,30). The isoflavone equol is expected to prevent hormone-dependent diseases, such as prostate cancer, because of its ability to bind to dihydrotestosterone and its high capacity to bind to estrogen receptor ␤; moreover, it is the most potent antioxidant of all the isoflavones (1, 2, 5, 17, 23).To date, several bacteria capable of producing equol have been isolated from human or animal feces (18-20, 29, 31). Many of these strains are suggested to first metabolize daidzein as a substrate to dihydrodaidzein (DHD) and to then metabolize DHD to equol. Recently, daidzein reductase, which converts daidzein to DHD, has been purified from the equol-producing Lactococcus strain 20-92 (25). On the other hand, it has been suggested that, in the Eggerthella strain Julong 732, DHD is converted to equol by the production of cis/trans-tetrahydrodaidzein (cis/trans-THD) as an intermediate metabolite (13,14). These studies have therefore suggested that daidzein is converted to equol via DHD and cis/ trans-THD. However, the details of the enzymes involved in the production of equol from daidzein, and of the genes encoding them, remain largely unknown.We have recently isolated Slackia sp. strain NATTS, which has potent daidzein-to-equol conversion ability, from healthy human feces (26). This strain has a more potent daidzein-equol conversion activity than the other equol-producing strains...

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of an Enzyme System for Daidzein-to-Equol Conversion in Slackia sp. Strain NATTS

Tsuji

Moriyama

Nomoto

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…On the basis of these sequence similarity data, the new genus Slackia was created for these two bacterial species, and E. exiguum and Peptostreptococcus heliotrinreducens were reclassified as S. exigua and S. heliotrinireducens, respectively. Since then, on the basis of phenotypic and phylogenetic analyses, four more species have been reported in the genus Slackia: S. faecicanis (15), S. isoflavoniconvertens (17), S. equolifaciens (11), and S. piriformis (19). S. exigua was isolated from human oral lesions (20); S. heliotrinireducens was isolated from sheep rumen (14); S. faecicanis was isolated from dog feces (15), and S. isoflavoniconvertens, S. equolifaciens, and S. piriformis were isolated from human feces (11,17,19).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Slackia exigua Isolated from Human Wound Infections, Including Abscesses of Intestinal Origin

Kim

Rowlinson²,

Bennion

et al. 2010

J Clin Microbiol

View full text Add to dashboard Cite

Eleven clinical strains isolated from infected wound specimens were subjected to polyphasic taxonomic analysis. Sequence analysis of the 16S rRNA gene showed that all 11 strains were phylogenetically related to Slackia exigua. Additionally, conventional and biochemical tests of 6 of the 11 strains were performed as supplementary methods to obtain phenotypic identification by comparison with the phenotypes of the relevant type strains. S. exigua has been considered an oral bacterial species in the family Coriobacteriaceae. This organism is fastidious and grows poorly, so it may easily be overlooked. The 16S rRNA gene sequences and the biochemical characteristics of four of the S. exigua strains isolated for this study from various infections indicative of an intestinal source were almost identical to those of the validated S. exigua type strain from an oral source and two of the S. exigua strains from oral sources evaluated in this study. Thus, we show for the first time that S. exigua species can be isolated from extraoral infections as well as from oral infections. The profiles of susceptibility to selected antimicrobials of this species were also investigated for the first time.

show abstract

“…Adlercreutzia equolifaciens MT4s-5 was isolated from rat feces as reported in our previous paper. 5) Adlercreutzia equolifaciens JCM 14793, 7) Asaccharobacter celatus JCM 14811, 8,9) Slackia equolifaciens JCM 16059, 10,11) and Slackia isoflavoniconvertens JCM 16137 12) were purchased from Riken Bioresource Center (Ibaraki, Japan). All other chemicals were available products of analytical or HPLC grade.…”

Section: Methodsmentioning

confidence: 99%

“…In this report, we describe the abilities of several commercially available isoflavone-metabolizing bacteria [7][8][9][10][11][12] to degrade EGC and its isomer GC. Furthermore, we note the p-dehydroxylation abilities of EGC metabolites, 3 and 4 by these bacteria.…”

mentioning

confidence: 99%

Biotransformation of (−)-Epigallocatechin and (−)-Gallocatechin by Intestinal Bacteria Involved in Isoflavone Metabolism

Takagaki¹,

Nanjo²

2015

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

Isolation of a Human Intestinal Bacterium Capable of Daidzein and Genistein Conversion

Cited by 139 publications

References 40 publications

Identification of an Enzyme System for Daidzein-to-Equol Conversion in Slackia sp. Strain NATTS

Identification of an Enzyme System for Daidzein-to-Equol Conversion in Slackia sp. Strain NATTS

Characterization of Slackia exigua Isolated from Human Wound Infections, Including Abscesses of Intestinal Origin

Biotransformation of (−)-Epigallocatechin and (−)-Gallocatechin by Intestinal Bacteria Involved in Isoflavone Metabolism

Contact Info

Product

Resources

About