ABSTRACT. Two field isolates of feline herpesvirus type 1 (FHV-1) designated as 00-015 and 00-035, were obtained from cats diagnosed as feline viral rhinotracheitis (FVR) in Japan. To analyze the character of recent FHV-1, these two isolates and our laboratory strain C7301 were inoculated experimentally to specific-pathogen-free cats. Although all cats showed typical FVR symptoms, more severe clinical symptoms were observed on cats infected with the isolates 00-015 and 00-035 compared with those of C7301-infected cats. Severe ocular lesions including conjunctivitis were found in the cats infected with the isolates, indicating that the recent FHV-1 has a potential to induce severe FVR symptoms including ocular lesions. KEY WORDS: conjunctivitis, feline herpesvirus type 1, feline viral rhinotracheitis.J. Vet. Med. Sci. 65(8): 939-943, 2003 Feline herpesvirus type 1 (FHV-1), a member of the subfamily Alphaherpesvirinae of the family Herpesviridae, causes an upper respiratory tract disease known as feline viral rhinotracheitis (FVR), characterized by upper respiratory symptoms such as sneezing, ocular and nasal discharges, and conjunctivitis [20,30,32,33,35]. Infection in newborn or debilitated cats with FHV-1 can result in a severe generalized disease with high mortality rate [32]. Most of cats that recover from acute FHV-1 infection become viral carriers [14].In 1958, FHV-1 was first isolated from kittens showing an upper respiratory syndrome. This original isolate, designated as C-27, is the prototype strain of FHV-1 [4]. Since then, many FHV-1 strains have been isolated and the clinical, virological, immunological and pathological features on FVR have been reported [8-12, 16, 19, 21-27, 29, 37]. We classified various field isolates of FHV-1 into 3 genotypes (F2, C7301 and C7805) using restriction endonuclease MluI cleavage pattern [22]. In the study, 8 of 80 field isolates were revealed to be the genotype of the attenuated vaccine strain F2. However, the pathogenicity of these genotypes is still to be analyzed.In 2000, two isolates of FHV-1 (00-015 and 00-035) were obtained from ocular discharges of cats diagnosed as FVR in Tokyo and Chiba Prefecture in Japan. These cats showed severe upper respiratory tract symptoms and conjunctivitis. In both cases, feline calicivirus and Chlamydia psittaci were not detected in the ocular swab samples by the methods described previously [27]. To evaluate the pathogenicity of these strains in vivo, we inoculated the two isolates and our laboratory strain C7301 [25] to specificpathogen-free (SPF) cats.The field isolates were propagated less than five times in Crandell feline kidney (CRFK) cell cultures [6]. MluI cleavage patterns of the genome of the isolates were determined by the methods described by Horimoto et al. [16]. The cells were grown in Dulbecco's modified Eagle's minimum essential medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum (FCS) and antibiotics. The maintenance medium for harvesting the virus was free from FCS.To determine the titer of ...