Isolation of a DNA sequence related to several plasmids from Bacillus thuringiensis after a mating involving the Streptococcus faecalis plasmid pAMβ1

Lereclus, Didier; Menou, Ghislaine; Lecadet, Marguerite-M.

doi:10.1007/bf00334831

Cited by 70 publications

(40 citation statements)

References 16 publications

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“…The bacterial strains Kpn223 and Kpn555 were strikingly different as recipients, prompting a comparison of the strains. The number of plasmids and type of replicon differed between strains Kpn223 and Kpn555, but the role of the number of recipient plasmids on conjugation efficiency is inconclusive (51)(52)(53). Kpn223 seems to carry only a plasmid with limited presumptive transfer genes and unclear conjugal ability, which may explain, in part, its high-efficiency recipient ability.…”

Section: Discussionmentioning

confidence: 99%

Horizontal Transfer of Carbapenemase-Encoding Plasmids and Comparison with Hospital Epidemiology Data

Hardiman

Weingarten

Conlan

et al. 2016

Antimicrob Agents Chemother

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dCarbapenemase-producing organisms have spread worldwide, and infections with these bacteria cause significant morbidity. Horizontal transfer of plasmids carrying genes that encode carbapenemases plays an important role in the spread of multidrug-resistant Gramnegative bacteria. Here we investigate parameters regulating conjugation using an Escherichia coli laboratory strain that lacks plasmids or restriction enzyme modification systems as a recipient and also using patient isolates as donors and recipients. Because conjugation is tightly regulated, we performed a systematic analysis of the transfer of Klebsiella pneumoniae carbapenemase (bla KPC )-encoding plasmids into multiple strains under different environmental conditions to investigate critical variables. We used four bla KPC -carrying plasmids isolated from patient strains obtained from two hospitals: pKpQIL and pKPC-47e from the National Institutes of Health, and pKPC_UVA01 and pKPC_UVA02 from the University of Virginia. Plasmid transfer frequency differed substantially between different donor and recipient pairs, and the frequency was influenced by plasmid content, temperature, and substrate, in addition to donor and recipient strain. pKPC-47e was attenuated in conjugation efficiency across all conditions tested. Despite its presence in multiple clinical species, pKPC_UVA01 had lower conjugation efficiencies than pKpQIL into recipient strains. The conjugation frequency of these plasmids into K. pneumoniae and E. coli patient isolates ranged widely without a clear correlation with clinical epidemiological data. Our results highlight the importance of each variable examined in these controlled experiments. The in vitro models did not reliably predict plasmid mobilization observed in a patient population, indicating that further studies are needed to understand the most important variables affecting horizontal transfer in vivo.

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Section: Discussionmentioning

confidence: 99%

Horizontal Transfer of Carbapenemase-Encoding Plasmids and Comparison with Hospital Epidemiology Data

Hardiman

Weingarten

Conlan

et al. 2016

Antimicrob Agents Chemother

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“…There are reports in the literature presenting evidence of repetitive sequences and transposonlike elements present on B. thuringiensis plasmids (18,(20)(21)(22)(23)(24). Therefore, the variant plasmids might result from transposition of sequences from B. thuringiensis plasmids to pXO1.…”

Section: Discussionmentioning

confidence: 99%

Identification of self-transmissible plasmids in four Bacillus thuringiensis subspecies

1987

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The transfer of plasmids by mating from four Bacillus thuringiensis subspecies to Bacillus anthracis and Bacillus cereus recipients was monitored by selecting transcipients which acquired plasmid pBC16 (Tcr). Transcipients also inherited a specific large plasmid from each B. thuringiensis donor at a high frequency along with a random array of smaller plasmids. The large plasmids (ca. 50 between the presence of these plasmids in a strain and its corresponding donor ability. The results indicate that these large B. thuringiensis plasmids are self-transmissible plasmids which can also mobilize pBC16 and other plasmids. Several of the B. thuringiensis self-transmissible plasmids isolated in our laboratory were examined for DNA homology and entry exclusion effects, and evidence that the plasmids are distinct but related is presented. The

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“…Studies by Lereclus et al (12,13) have shown that Tn4430 promotes deletions in DNA sequences adjacent to its endpoints. It is possible then that Tn4430 or insertion elements related to IR1 are responsible for the spontaneous loss of DNA sequences on this region of pXO12.…”

Section: Discussionmentioning

confidence: 99%

Involvement of Tn4430 in transfer of Bacillus anthracis plasmids mediated by Bacillus thuringiensis plasmid pXO12

1989

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The self-transmissible plasmid pXO12 (112.5 kilobases [kbj), originally isolated from strain 4042A of BaciUus thuringiensis subsp. thuringiensis, codes for production of the insecticidal crystal protein (Cry'). The mechanism of pXO12-mediated plasmid transfer was investigated by monitoring the cotransfer of the tetracycline resistance plasmid pBC16 (4.2 kb) and the Bacillus anthracis toxin and capsule plasmids, pXOl (168 kb) and pXO2 (85.6 kb), respectively. In matings of B. anthracis donors with B. anthracis and Bacillus cereus recipients, the number of Tcr transcipients ranged from 4.8 x 104 to 3.9 x 106/ml (frequencies ranged from 1.6 x 10-4 to 7.1 x 10-2), and 0.3 to 0.4% of them simultaneously inherited pXO1 or pXO2. Physical analysis of the transferred plasmids suggested that pBC16 was transferred by the process of donation and that the large B. anthracis plasmids were transferred by the process of conduction. The transfer of pXO1 and pXO2 involved the transposition of Tn4430 from pXO12 onto these plasmids. DNA-DNA hybridization experiments demonstrated that Tn4430 was located on a 16.0-kb AvaI fragment of pXO12. Examination of Tra-and Cryderivatives of pXO12 showed that this fragment also harbored information involved in crystal formation and was adjacent to a restriction fragment containing DNA sequences carrying information required for conjugal transfer.Recent reports from our laboratory have concerned the identification and characterization of six self-transmissible plasmids from five subspecies of Bacillus thuringiensis (1,19). One of these plasmids, designated pXO12 (112.5 kilobases [kb]), was isolated from strain 4042A of B. thuringiensis subsp. thuringiensis. Plasmid pXO12 is capable of mediating its own transfer as well as that of a large range of Bacillus plasmids among strains of Bacillus anthracis, Bacillus cereus, and B. thuringiensis. In addition to conjugal transfer functions, pXO12 also encodes production of the insecticidal toxin known as the 8-endotoxin or the parasporal crystal (Cry').The widespread occurrence of large self-transmissible plasmids among B. thuringiensis strains suggests that conjugation may be an important means of plasmid dissemination in naturally occurring Bacillus populations (4). In the laboratory, this mating system has provided us with an efficient method of shuttling a wide range of plasmids among B. thuringiensis, B. cereus and B. anthracis. For example, the utility of this conjugation system in the genetic analysis of the B. anthracis toxin and capsule plasmids, pXO1 and pXO2, respectively, has been well documented (1,6,19,24).Prior to the development of this mating system, the most reliable system available for transferring plasmids among these three Bacillus species was transduction (21). Therefore, the importance of this system as an additional means of genetic exchange among these organisms warrants further genetic and physical analysis of these conjugative plasmids. We have been interested in determining the mechanism of pXO12-mediated transfer of both lar...

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Isolation of a DNA sequence related to several plasmids from Bacillus thuringiensis after a mating involving the Streptococcus faecalis plasmid pAMβ1

Cited by 70 publications

References 16 publications

Horizontal Transfer of Carbapenemase-Encoding Plasmids and Comparison with Hospital Epidemiology Data

Horizontal Transfer of Carbapenemase-Encoding Plasmids and Comparison with Hospital Epidemiology Data

Identification of self-transmissible plasmids in four Bacillus thuringiensis subspecies

Involvement of Tn4430 in transfer of Bacillus anthracis plasmids mediated by Bacillus thuringiensis plasmid pXO12

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