Isolation of a Continuous Epithelioid Cell Line, HBT-3, from a Human Breast Carcinoma

Bassin, Robert H.; Plata, Ernest J.; Gerwin, Brenda I.; Mattern, Carl F. T.; Haapala, Daniel K.; Chu, Elizabeth W.

doi:10.3181/00379727-141-36850

Cited by 35 publications

(8 citation statements)

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“…Several different mouse tissue culture lines were employed as target cells: R B L J , a Rauscher murine leukemia virus (R-MuLV)-induced lymphoma (Herberman et al, 1974), and MBL-2, a Moloney murine leukemia virus (M-MuL)-induced lymphoma of B6 mice (Herberman et al, 1974); YAC-1, an M-MuLV-induced lymphoma of A/Sn mice (Herberman et al, 1974); RL61, a radiation-induced lymphoma of BALBic mice (Sendo et al, 1975); BALW 3T3, M-MuLV-infected BALB/3T3, mKSA-TU-5 (TU-5) tumor (Lozzio and Lozzio, 1973), a kidney line transformed by simian virus 40 (SV40) of BALB/c mice; and P815 mastocytoma of DBA/2 mice Herberman et al, 1974). One human myeloid leukemia line, K562, was also tested (Bassin et al, 1973) All cells were maintained in culture using…”

Section: Target Cellsmentioning

confidence: 99%

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Cytolytic activity against tumor cells by macrophage cell lines and augmentation by macrophage stimulants

Taniyama

Holden

1980

Intl Journal of Cancer

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Previous studies have shown that macrophage cell lines retained the ability to phagocytize, to secrete lysosomal enzymes, and to function as effector cells in antibody-dependent cellular cytoxicity. In this paper, the cytolytic activity of murine macrophage cell lines against tumor target cells was assessed using an 18-h 51Cr release assay. Of the macrophage cell lines tested, RAW 264, PU5-1.8 and IC-21 had intermediate to high levels of spontaneous cytolytic activity, P388D, and J774 had low to intermediate levels, while /WEHI-3 showed little or no cytolytic activity against RBL-5, MBL-2 and TU-5 target cells. Tumor-cell killing by macrophage cell lines could be augmented by the addition of macrophage stimulants, such as bacterial lipopolysaccharide and poly I:C, indicating that the activation of macrophages by these stimulants does not require the participation of other cell types. Treatment with interferon also augmented the tumor-cell killing by macrophage cell lines. Although the mechanism by which these cell lines exert their spontaneous or boosted cytotoxic activity is not clear, it does not appear to be due to depletion of nutrients since cell lines with high metabolic and proliferative activities, such as WEHI-3 and RBL-5, showed little or no cytotoxicity and supernatants from the macrophage cell lines did not exert any cytotoxic effects in their essay. Thus, it appears that the different macrophage cell lines represent different levels of activation and/or differentiation and may be useful for studying the development of these processes as well as providing a useful tool for analyzing the mechanisms of macrophage-mediated cytolysis.

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Section: Target Cellsmentioning

confidence: 99%

“…One human myeloid leukemia line, K562, was also tested (Bassin et al, 1973) All cells were maintained in culture using…”

Section: Target Cellsmentioning

confidence: 99%

Cytolytic activity against tumor cells by macrophage cell lines and augmentation by macrophage stimulants

Taniyama

Holden

1980

Intl Journal of Cancer

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“…Breast cancer and brain tumours were the first two solid malignancies in which stem cells were described [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

An update on the biology of cancer stem cells in breast cancer

et al. 2008

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Breast cancer stem cells are defined as cancer cells with self-renewal capacity. These cells represent a small subpopulation endowed with the ability to form new tumours when injected in nude mice. Markers of differentiation have been used to identify these cancer cells. In the case of breast cancer, CD44+/CD24- select a population with stem cell properties. The fact that these cells have self-renewal ability has suggested that this population could be responsible for new tumour formation and cancer relapse. These cells have been shown to be more resistant to chemotherapy and radiotherapy than normal cancer cells. The identification of the molecular druggable alterations responsible for the initiation and maintenance of cancer stem cells is an important goal. In this article we will review all these points with special emphasis on the possible role of new drugs designed to interact with molecular pathways of cancer stem cells.

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“…Both are secretory epithelial cell lines (10). The other four were unsatisfactory for the following reasons: HBT 3 (22) and HBT 39B (23) were found to be HeLa cells (8). 734B (24) had growth characteristics that produced unreproducible day-to-day results.…”

Section: The Assaymentioning

confidence: 99%

Tumor-specific cell-mediated immunity in household contacts of cancer patients..

Byers¹,

Levin²,

Hackett³

et al. 1975

J. Clin. Invest.

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A B S T R A C T Patients with osteogenic sarcoma (and related tumors), hypernephroma, and breast carcinoma, and their household contacts were tested for tumorspecific cell-mediated immunity against these tumors with the use of a short-term chromium-51 release assay. This assay, reproducible over many months and well-correlated with the clinical course of the patients, was used to demonstrate that household contacts of patients with osteogenic sarcoma and breast carcinoma have specific immunity against the tumor type with which they have been in contact. In both types of tumors, the range of cytotoxicity values produced by lymphocytes from the household contacts was significantly higher than that of the normal population. The incidence of immunity was much higher in household contacts of patients with breast carcinoma than in those of patients with osteogenic sarcoma. Immunity was found with equal frequency in men and women, as well as in genetically and nongenetically related household contacts (guardians, adopted children, spouses).

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Isolation of a Continuous Epithelioid Cell Line, HBT-3, from a Human Breast Carcinoma

Cited by 35 publications

References 0 publications

Cytolytic activity against tumor cells by macrophage cell lines and augmentation by macrophage stimulants

Cytolytic activity against tumor cells by macrophage cell lines and augmentation by macrophage stimulants

An update on the biology of cancer stem cells in breast cancer

Tumor-specific cell-mediated immunity in household contacts of cancer patients..

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