Isolation of a cinnamoyl CoA reductase gene involved in formation of stone cells in pear (Pyrus pyrifolia)

Lu, Xiaopeng; Liu, Yong-Zhong; An, Ji-Cui; Hu, Hongju; Peng, Shuang

doi:10.1007/s11738-010-0583-x

Cited by 10 publications

(9 citation statements)

References 28 publications

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“…The levels of PbCCOMT3 expression between 47 and 63 DAF were higher than those of PbCCOMT1. These results are consistent with the trend observed in pear for lignin and stone cell contents (Cai et al, 2010;Lu et al, 2011), indicating that the two candidate CCOMT genes are probably involved in stone cell development and lignin biosynthesis in DangShan Su pear fruit.…”

Section: Identification and Spatio-temporal Expression Analysis Of Casupporting

confidence: 90%

“…During the formation of stone cells, lignin is deposited in the corner of the primary wall of parenchyma cells, from where it extends to the intercellular and secondary wall layers along the primary wall corner until the whole cell lumen is covered. Ultimately, a solid stone cell is formed (Lu et al, 2011;Jin et al, 2013). Therefore, lignin biosynthesis is thought to be involved in the generation of enlarged stone cells in pear fruit and in their presence in increased numbers.…”

Section: Introductionmentioning

confidence: 99%

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Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Cheng

Xiong

et al. 2016

Genet. Mol. Res.

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ABSTRACT. With high nutritional value in its fruits, Dangshan Su pear has been widely cultivated in China. The stone cell content in fruits is a key factor affecting fruit quality in pear, and the formation of stone cells has been associated with lignin biosynthesis. O-Methyltransferase (OMT) is a key enzyme involved in lignin metabolism within the phenylpropanoid pathway. Here, we screened 26 OMT genes from the Pyrus bretschneideri cv. Dangshan Su genome using the DNATOOLs software. To characterize the OMT gene family in pear, gene structure, chromosomal localization, and conserved motifs of PbOMTs were analyzed. PbOMTs were divided into two categories, type I (designated PbCCOMTs) and type II (designated PbCOMTs), indicating the differentiation of function during evolution. Based on the analysis of multiple sequence alignment, cis-element prediction, and phylogenetic relationships, two candidate genes, PbCCOMT1 and PbCCOMT3, were selected for the analysis of temporal and spatial gene expression in pear. The promoter regions of both PbCCOMT1 and PbCCOMT3 contain regulatory motifs for lignin synthesis. Moreover, the two genes show high similarity and close phylogenetic relationships with CCOMTs in other species. Expression analysis showed that transcript levels of two PbCCOMTs were positively associated with the contents of both stone cells and lignin during the development of pear fruit. These results suggest that PbCCOMT1 and PbCCOMT3 are closely associated with lignin biosynthesis. These findings will help clarify the function of PbOMTs in lignin metabolism and to elucidate the mechanisms underlying stone cell formation in pear.

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Section: Identification and Spatio-temporal Expression Analysis Of Casupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Cheng

Xiong

et al. 2016

Genet. Mol. Res.

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“…The primary purpose of the present study was to compare gene expression patterns in loquat fruits stored at 0 °C after an HT or LTC treatment, both of which cause significantly reduced lignification, in order to identify structural and regulatory genes that contribute to loquat fruit lignification during cold storage. Some studies have focused on structural genes to understand the lignin regulation pathway in fruits, such as CCR in pear 38 and 4CL in mulberry 39 . Here we have also identified several structural genes, such as those encoding PAL, 4CL, COMT, HCT, and CAD, that contribute to the lignification of loquat fruits during cold storage (Fig.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis provides insights into the regulation of metabolic processes during postharvest cold storage of loquat (Eriobotrya japonica) fruit

et al. 2019

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Loquat ( Eriobotrya japonica ) fruit accumulates lignin during postharvest storage under chilling conditions (0 °C), while low-temperature conditioning (LTC; 5 °C for 6 days followed by transfer to 0 °C) or heat treatment (HT; 40 °C for 4 h followed by transfer to 0 °C) can alleviate lignification. Here we compared transcriptome profiles of loquat fruit samples under LTC or HT to those stored at 0 °C at five time points from day 1 to day 8 after treatment. High-throughput transcriptome sequences were de novo assembled into 53,319 unique transcripts with an N50 length of 1306 bp. A total of 2235 differentially expressed genes were identified in LTC, and 1020 were identified in HT compared to 0 °C. Key genes in the lignin biosynthetic pathway, including EjPAL2 , EjCAD1 , EjCAD3 , 4CL , COMT , and HCT , were responsive to LTC or HT treatment, but they showed different expression patterns during the treatments, indicating that different structural genes could regulate lignification at different treatment stages. Coexpression network analysis showed that these candidate biosynthetic genes were associated with a number of transcription factors, including those belonging to the AP2, MYB, and NAC families. Gene ontology (GO) enrichment analysis of differentially expressed genes indicated that biological processes such as stress responses, cell wall and lignin metabolism, hormone metabolism, and metal ion transport were significantly affected under LTC or HT treatment when compared to 0 °C. Our analyses provide insights into transcriptome responses to postharvest treatments in loquat fruit.

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“…It was reported that lignin plays a key role in the formation of stone cells. Lignin is deposited on the cell walls of pear fruit and makes the secondary cell wall thicker (Cai et al, 2010, Choi et al, 2007, Jin et al, 2013, Lu et al, 2011, Tao et al, 2009). The increase in stone cell content leads to tissue lignification, which can influence fruit texture and postharvest handling (Cai et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Cinnamate-4-Hydroxylase Gene Is Involved in the Step of Lignin Biosynthesis in Chinese White Pear

Tao

Wang

Jin

et al. 2015

J. Amer. Soc. Hort. Sci.

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Lignin is the main component of stone cells, and stone cell content is one of the crucial factors for fruit quality in chinese white pear (Pyrus ×bretschneideri). The lignin biosynthesis pathway is complex and involves many enzymatic reactions. Cinnamate-4-hydroxylase [C4H (EC.1.14.13.11)] is an essential enzyme in lignin metabolism. This study was conducted to investigate the effect of bagging on lignin metabolism during fruit development in chinese white pear. The study showed that bagging had little effect on stone cell content, lignin content, C4H activity, and C4H gene expression and that there was a positive correlation between C4H gene expression and lignin content as well as stone cell content. Moreover, a full-length complementary DNA (cDNA) encoding C4H (PbrC4H, GenBank accession number KJ577541.1) was isolated from chinese white pear fruit. The cDNA is 1515 bp long and encodes a protein of 504 amino acids. Sequence alignment suggested that the deduced protein belongs to the P450 gene family and that C4H might be located subcellularly in the cell membrane. The results indicate that bagging cannot change the lignin and stone cell content significantly and that C4H catalyzes a step in lignin biosynthesis. These findings provide certain theoretical references and practical criteria for improving the quality of chinese white pear.

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Isolation of a cinnamoyl CoA reductase gene involved in formation of stone cells in pear (Pyrus pyrifolia)

Abstract: Stone cell which appears in most pear cultivars contributes badly to pear internal quality.

Cited by 10 publications

References 28 publications

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Transcriptome analysis provides insights into the regulation of metabolic processes during postharvest cold storage of loquat (Eriobotrya japonica) fruit

Cinnamate-4-Hydroxylase Gene Is Involved in the Step of Lignin Biosynthesis in Chinese White Pear

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