Isolation of 8-methoxypsoralen accessible DNA domains from chromatin of intact cells

Mathis, Georg A.; Althaus, Felix R.

doi:10.1007/bf00135025

Cited by 12 publications

(5 citation statements)

References 31 publications

(42 reference statements)

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“…Thus, the magnitude of chromatin rearrangements is a critical issue. Specifically, despite the short size of the NER patch (~30 nucleotides in length) [46], chromatin disorganization can extend several kilobases away from the damage site, as shown by probing psoralen accessibility of chromatin in rat hepatocytes upon induction of bulky DNA adducts [47]. Furthermore, relaxation of chromatin affecting the whole nucleus has been reported in response to UVC irradiation of a limited nuclear area.…”

Section: Access/prime Stepmentioning

confidence: 99%

Chromatin dynamics after DNA damage: The legacy of the access–repair–restore model

2015

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Eukaryotic genomes are packaged into chromatin, which is the physiological substrate for all DNA transactions, including DNA damage and repair. Chromatin organization imposes major constraints on DNA damage repair and thus undergoes critical rearrangements during the repair process. These rearrangements have been integrated into the "Access-Repair-Restore" (ARR) model, which provides a molecular framework for chromatin dynamics in response to DNA damage. Here, we take a historical perspective on the elaboration of this model and describe the molecular players involved in damaged chromatin reorganization in human cells. In particular, we present our current knowledge of chromatin assembly coupled to DNA damage repair, focusing on the role of histone variants and their dedicated chaperones. Finally, we discuss the impact of chromatin rearrangements after DNA damage on chromatin function and epigenome maintenance.

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Section: Access/prime Stepmentioning

confidence: 99%

Chromatin dynamics after DNA damage: The legacy of the access–repair–restore model

2015

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“…Remarkably, these rearrangements can span several kilobases on chromatin [20] despite the small size the repair patch, which is around 30 nucleotides for Nucleotide Excision Repair (NER) of UVC damage in human cells [21]. Furthermore, a global relaxation of chromatin affecting the whole nucleus has been reported in response to local UVC irradiation, as highlighted by an increased sensitivity of chromatin to denaturation by hydrochloric acid [22].…”

Section: Monitoring Histone Dynamics In Response To Dna Damage In Hummentioning

confidence: 99%

Reshaping Chromatin after DNA Damage: The Choreography of Histone Proteins

Polo

2015

Journal of Molecular Biology

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DNA damage signaling and repair machineries operate in a nuclear environment, where DNA is wrapped around histone proteins and packaged into chromatin. Understanding how chromatin structure is restored together with the DNA sequence during DNA damage repair has been a topic of intense research. Indeed, chromatin integrity is central to cell functions and identity. Yet, chromatin shows remarkable plasticity in response to DNA damage. This review presents our current knowledge of chromatin dynamics in the mammalian cell nucleus in response to DNA-double strand breaks and UV lesions. I provide an overview of the key players involved in regulating histone dynamics in damaged chromatin regions, focusing on histone chaperones and their concerted action with histone modifiers, chromatin remodelers and repair factors. I also discuss how these dynamics contribute to reshaping chromatin and, by altering the chromatin landscape, may affect the maintenance of epigenetic information.

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“…). Such rearrangements of chromatin may extend up to several kilobases from UV lesions , even though the size of the oligonucleotide excised during UV damage repair in vivo is only approximately 30 nucleotides . Chromatin rearrangements in response to DNA damage probably involve nucleosome dynamics (i.e.…”

Section: Chromatin Dynamics In Response To Dna Damagementioning

confidence: 99%

How to restore chromatin structure and function in response to DNA damage – let the chaperones play

2014

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Histone deposition onto DNA assisted by specific chaperones forms the chromatin basic unit and serves to package the genome within the cell nucleus. The resulting chromatin organization, often referred to as the epigenome, contributes to a unique transcriptional program that defines cell identity. Importantly, during cellular life, substantial alterations in chromatin structure may arise due to cell stress, including DNA damage, which not only challenges the integrity of the genome but also threatens the epigenome. Considerable efforts have been made to decipher chromatin dynamics in response to genotoxic stress, and to assess how it affects both genome and epigenome stability. Here, we review recent advances in understanding the mechanisms of DNA damage‐induced chromatin plasticity in mammalian cells. We focus specifically on the dynamics of histone H3 variants in response to UV irradiation, and highlight the role of their dedicated chaperones in restoring both chromatin structure and function. Finally, we discuss how, in addition to restoring chromatin integrity, the cellular networks that signal and repair DNA damage may also provide a window of opportunity for modulating the information conveyed by chromatin.

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Isolation of 8-methoxypsoralen accessible DNA domains from chromatin of intact cells

Cited by 12 publications

References 31 publications

Chromatin dynamics after DNA damage: The legacy of the access–repair–restore model

Chromatin dynamics after DNA damage: The legacy of the access–repair–restore model

Reshaping Chromatin after DNA Damage: The Choreography of Histone Proteins

How to restore chromatin structure and function in response to DNA damage – let the chaperones play

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