Isolation of 5-hydroxyuridine (iso-barbituridine) from yeast ribonucleic acids

Lis, Adam W.; Passarge, Walter E.

doi:10.1016/0003-9861(66)90384-5

Cited by 18 publications

(3 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3) was also submitted to cold and 3H-postlabel ing analysis. However, ho5U is rather unstable during the processes of column and thin layer chromatographies (23) and desalting procedure and also oxidation-reduction during postlabeling analysis. Therefore ho5U was only obtained in detectable amounts when ho5U-containing tRNA was directly analyzed.…”

Section: Nd Trnavoimentioning

confidence: 99%

On the biosynthesis of 5-methoxyuridine and uridine-5-oxyacetic acid in specific procaryotic transfer RNAs

Murao¹,

Ishikura²,

Albani³

et al. 1978

Nucl Acids Res

View full text Add to dashboard Cite

The uridine-5-0-derivatives, 5-methoxyuridine (mo5U) and uridine-5-oxyacetic acid (cmo5U) occupy the first position of anticondons in certain tRNA species of B. subtilis and E. coli, respectively. Here we present experimental evidence showing that both modifications are derived from a common precursor, 5-hydroxyuridine. Incompletely modified tRNASer and tRNAVal from E. coli met- rel-. All five tRNAs accepted methyl groups from S-adenosylmethionine with B. subtilis extracts in vitro and mo5U was formed. In B. subtilis tRNAs the mo5U was proved to be at the specific site; in E. coli tRNAVal the mo5U was demonstrated to be present in the oligonucleotide that comprises the anticodon. In submethylated E. coli tRNAVal,5-hydroxyuridine was detected whereas considerable amounts of cmo5U were lacking.

show abstract

Section: Nd Trnavoimentioning

confidence: 99%

On the biosynthesis of 5-methoxyuridine and uridine-5-oxyacetic acid in specific procaryotic transfer RNAs

Murao¹,

Ishikura²,

Albani³

et al. 1978

Nucl Acids Res

View full text Add to dashboard Cite

show abstract

“…However, as soon as the CD ring is open, β -amylase will rapidly degrade the linear oligosaccharides to G1, if the substrate contains an even number of glucose units, or to G2 plus G3 and glucose if the oligosaccharide contains an odd number of units [74] . The γ -CD was treated with crystalline porcine pancreatic α -amylase together with a 60 -fold excess of crystalline sweetpotato β -amylase.…”

Section: Enzymatic Synthesis and Degradation Of Cyclodextrin 401mentioning

confidence: 99%

Enzymatic Polysaccharide Degradation

Munteanu¹,

Ritter²

2010

Biocatalysis in Polymer Chemistry

View full text Add to dashboard Cite

show abstract

“…[8][9][10] The RNA analogue, 5-hydroxyuridine has been synthesized 11 and later isolated from yeast as a modified minor RNA nucleoside. 12 5-Hydroxy-2′-deoxyuridine (ho 5 dUrd) has been a subject of a number of computational and experimental investigations. ho 5 dUrd exists as a 5-hydroxy enol tautomer forming an intramolecular hydrogen bond with the carbonyl group in position 4 as evidenced by UV, X-ray data and computational studies.…”

Section: Introductionmentioning

confidence: 99%

The evaluation of 5-amino- and 5-hydroxyuracil derivatives as potential quadruplex-forming agents

et al. 2017

View full text Add to dashboard Cite

5-Substituted uracils (NH or OH groups in position 5) have been examined theoretically and experimentally as potential building blocks in quadruplex structures. Our high level Density Functional Theory (DFT) calculations showed that the tetramer formation and stacking energies for 5-substituted uracils are similar to the energies of purine-based xanthine (X) or guanine (G) structures. As tetrads of 5-substituted uracils cover almost exactly the same area as purine tetrads, mixed tetrads or quadruplex structures based on X or G and 5-substituted uracil motifs are possible. According to the calculations, 5-hydroxyuracil-based structures are the best candidates for experimental implementation which was corroborated by the existence of higher complexes in the mass spectra of 1-benzyl-5-hydroxyuracil. These pyrimidine-based molecules can be used as efficient building blocks in different applications including aptamers, bio-sensors or - taking into account the larger cavity in the central region of 5-hydroxyuracil structures - as an artificial ion channel.

show abstract

Isolation of 5-hydroxyuridine (iso-barbituridine) from yeast ribonucleic acids

Cited by 18 publications

References 9 publications

On the biosynthesis of 5-methoxyuridine and uridine-5-oxyacetic acid in specific procaryotic transfer RNAs

On the biosynthesis of 5-methoxyuridine and uridine-5-oxyacetic acid in specific procaryotic transfer RNAs

Enzymatic Polysaccharide Degradation

The evaluation of 5-amino- and 5-hydroxyuracil derivatives as potential quadruplex-forming agents

Contact Info

Product

Resources

About