Isolation, molecular identification, and phylogenetic evaluation of Cryptococcus neoformans isolated from pigeon lofts, Psittaciformes, and Passeriformes in Ahvaz, Iran

Mirpourian, S. S.; Sharifi, Neda; Talazadeh, Forough; Jafari, Ramezan Ali; Ghorbanpoor, Masoud

doi:10.1016/j.cimid.2021.101618

Cited by 4 publications

(5 citation statements)

References 43 publications

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“…These results are in agreement with a study by Mirpourian et al. ( 2021 ) that isolated C. neoformans from pigeons, parrots and sparrows in Ahvaz, Iran. They collected 80 samples from pigeon dropping, 163 samples from parrots and sparrows’ droppings, and 70 cloacal swabs from parrots and sparrows.…”

Section: Discussionsupporting

confidence: 93%

“…In this research, 105 fungal isolates were isolated from 79 samples and the most abundant isolated yeast was C. neoformans (17.14 %) (Table1). These results are in agreement with a study byMirpourian et al (2021) that isolated C. neoformans from pigeons, parrots and sparrows in Ahvaz, Iran. They collected 80 samples from pigeon dropping, 163 samples from parrots and sparrows' droppings, and 70 cloacal swabs from parrots and sparrows.…”

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confidence: 93%

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Fungal isolation and identification from parrot excreta in northeast Iran: A threat to human health

Malekifard

Ghaniei

Eidi

2023

Veterinary Medicine & Sci

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Background: One of the environmental sources of pathogenic fungi is parrot droppings.Objectives: Thus, this work aimed to investigate fungal contamination in parrots' droppings.Methods: Seventy-nine parrot droppings including Cockatiel, Cockatoo, Green check conure, Love bird, Budgerigar, African grey parrot, Alexandrine parakeet, Amazon parrot, Yellow crown parakeet, and Macaw were sampled, and suspended 1:10 in saline solution and then 0.5 mL of supernatant was cultured. The fungi were identified by standard mycological techniques.Results: Fungal contamination was detected in 66 samples (83.54%) out of 79 samples.Yeast and mould fungi were respectively isolated from 44 samples (55.69%) and 36 samples (45.56%) out of 79 samples. Overall 105 fungal isolates were isolated from parrot excreta. Cryptococcus neoformans (17.14%), Rhizopus spp. (10.47%), Rhodotorula spp. and Aspergillus niger (6.66%), and Penicillium spp. (5.71%) were the most fungi isolated from faecal samples. Conclusions:The evidence from this study suggests that the rate of fungal contamination in parrots' excreta was high. Keeping parrots in the house and their close contact with humans can double the importance of these contaminations and provide the basis for the transmission of contamination to humans. Therefore, the accumulations of parrot faeces for long periods suggest a potential threat to public health.

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Section: Discussionsupporting

confidence: 93%

supporting

confidence: 93%

Fungal isolation and identification from parrot excreta in northeast Iran: A threat to human health

Malekifard

Ghaniei

Eidi

2023

Veterinary Medicine & Sci

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“…As expected, C. neoformans was easily isolated by direct culture of pigeon dropping ( Chae et al, 2012a , b ; Soltani et al, 2013 ; Ellabib et al, 2016 ; Ghaderi et al, 2019 ; Pakshir et al, 2019 ; Mirpourian et al, 2021 ) and identification was confirmed by DNA sequencing. Sequence-based identification was confirmed by different ways.…”

Section: Discussionsupporting

confidence: 63%

“…2013; Ellabib et al, 2016;Ghaderi et al, 2019;Pakshir et al, 2019;Mirpourian et al, 2021) and identification was confirmed by DNA sequencing. Sequence-based identification was confirmed by different ways.…”

Section: Discussionmentioning

confidence: 89%

“…Due to the availability and affordability of better-resolution molecular biology techniques, such as DNA sequencing, more reports of infections with previously less reported Cryptococcus species become more evident ( Yong et al, 2016 ; Pakshir et al, 2019 ; Mirpourian et al, 2021 ). Other less common opportunistic Cryptococcus species are becoming an important cause of serious infections such as peritonitis and fungaemia in immunocompromised patients ( Ragupathi and Reyna, 2015 ; Aghaei Gharehbolagh et al, 2017 ; Choe et al, 2020 ; Rodriguez-Leguizamon et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Cryptococcus neoformans and Other Opportunistic Cryptococcus Species in Pigeon Dropping in Saudi Arabia: Identification and Characterization by DNA Sequencing

et al. 2021

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The prevalent variants of Cryptococcus neoformans, and other Cryptococcus species in pigeon excreta in Western Region of Saudi Arabia were studied. Ninety pigeon dropping samples were plated directly on Niger seed agar, and suspected colonies were sequenced using Illumina MiSeq. Species identification was determined using sequence read mapping to reference genomes of the two C. neoformans variants. In addition, sequence reads were identified using the KmerFinder tool. internal transcribed spacer 2 in the rDNA was also used for fungal barcoding of none of the C. neoformans species using two fungal identification databases. Phylogeny was studied using CSI Phylogeny (Center for Genomic Epidemiology, Denmark). The C. neoformans var. grubii mitochondrion and chromosome 1 reference sequences (accession numbers NC_004336.1 and CP022321.1, respectively) were used for sequence comparison and variant calling. Fifteen Cryptococcus isolates were isolated, 11 were identified as C. neoformans var. grubii, and 4 were found to be other opportunistic Cryptococcus species. Phylogeny analysis of C. neoformans var. grubii isolates showed a high degree of similarity between the C. neoformans isolates especially at the mitochondrial genome level. This study supports the fact that pathogenic and opportunistic Cryptococcus species are prevalent in domestic bird excreta which is an easy source of infection in the susceptible population.

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Preliminary characterization of the cutaneous fungal microbiota of domesticated Psittaciformes in Acre, Brazilian Amazon

Rezende,

da Rocha,

Bastos

et al. 2024

Ornithol. Res.

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Isolation, molecular identification, and phylogenetic evaluation of Cryptococcus neoformans isolated from pigeon lofts, Psittaciformes, and Passeriformes in Ahvaz, Iran

Cited by 4 publications

References 43 publications

Fungal isolation and identification from parrot excreta in northeast Iran: A threat to human health

Fungal isolation and identification from parrot excreta in northeast Iran: A threat to human health

Cryptococcus neoformans and Other Opportunistic Cryptococcus Species in Pigeon Dropping in Saudi Arabia: Identification and Characterization by DNA Sequencing

Preliminary characterization of the cutaneous fungal microbiota of domesticated Psittaciformes in Acre, Brazilian Amazon

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