Isolation, molecular characterization and pathogenicity studies of infectious bursal disease field virus isolates

Amer, M. M.; El-Bayomi, K. M.; Kotkat, M. Abd-A.; Abdel-Ghany, Wafaa A.; Shakal, Mohamed; Abdel-Gaied, Sherein S.

doi:10.21608/jvmr.2008.77841

Cited by 3 publications

(4 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Histopathologlogial lesions in IBDV infected groups showed severe depletion of the lymphoid follicle with activation of germinal center, deposition of intrafollicular connective tissues in examined bursa; while examined intestines showed depletion of the lymphoid follicle tissue and hypertrophy of the epithelial lining making finger like projection. These results agree with Dash et al (1991), Inoue et al (1994), Amer et al (2007), Singh et al (2015) and Ghetas et al (2022).…”

Section: Results Of Performance Parameters After Immunostimulantssupporting

confidence: 93%

“…The recorded signs and post mortem lesions in chicken groups challenged with virulent IBDV were similar to those previously reported (Amer et al, 2007;Eterradossi and Saif, 2013;Wagari, 2021;Ghetas et al, 2022). While, the recorded signs and post mortem lesions in chicken groups challenged with NDV VII.1.1 were the same to those previously mentioned by Amer et al (2018bAmer et al ( , 2019 and Ahmed et al (2022b).…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

The Effectiveness of Immunostimulants on NDV Genotype VII and IBDV Infection in Broiler Chickens

Ahmed¹,

Amer²,

El-Bayoumi³

et al. 2023

AAVS

View full text Add to dashboard Cite

The present study was conducted to investigate the pathogeneicity of Newcastle Disease Virus (NDV) genotype VII.1.1 "NDV-CHICKEN-EGY-ALEX-NRC-2020" strain in combination with Infectious Bursal Disease Virus (IBDV) "IBDV/Egypt/Qalubia/17" and/or some commercial immunostimulants (Lector ® and Orego ® ) in commercial broiler chickens. The pathogenicity studies parameters included the effects on protection, growth performance and clinico-pathological changes. The results indicated that, immunostimulants can keep maternal immunity longer where lector ® was the best; the decline in HI titers also indicates that bird groups not contract ND natural infection. The results of performance parameters after immunostimulants administration and challenged with IBDV at 14-days of age and NDV GenotypeVII.1.1 at 21-days of age in broiler chickens proved that administration of Lector ® and Orego ® solution had positive effect on average body weight gain and feed conversion rates than control non medicated groups; where the Lector medicated groups showed higher rates of average weight followed by Orego ® medicated groups. Regarding signs and mortality in infected groups IBD signs varied from mild in lector ® , moderate in Orego ® while sever signs were in non-medicated group. The mortalities began at 5 th day post-challenge (pch) in non-medicated groups with total 10-20%; while the mortalities in NDV began at 3 rd day pch in group non-medicated with 50% to reach 100% mortality rate in 7 th pch. At necropsy both IBD and NDV infection lesions were found in dead birds of all challenged groups. Chicken groups challenged by IBDV or NDV after treatment with Lector showed milder histopathological changes than Orego solution and non-treated groups showed the most sever lesions. Chicken groups challenged with both virulent IBDV and NDV either treatment with Lector ® or Orego ® solution or non-treated showed no marked difference in histopathological lesions. The results of total and differential leucocytes indicated that lector had a great high effect in WBCs levels specially lymphocytes and monocytes. In conclusion the used immunostimulants are of low value against IBD and ND virulent viruses challenge. In conclusion, is recommended to apply a strict hygienic measures and suitable vaccination programmes to protect chickens against both IBD and NDV infection as the current control and prevention regimes, including vaccines and vaccination protocol are not adequate against either single or mixed infection infections with IBD and/or ND.

show abstract

Section: Results Of Performance Parameters After Immunostimulantssupporting

confidence: 93%

Section: Discussionsupporting

confidence: 89%

The Effectiveness of Immunostimulants on NDV Genotype VII and IBDV Infection in Broiler Chickens

Ahmed¹,

Amer²,

El-Bayoumi³

et al. 2023

AAVS

View full text Add to dashboard Cite

show abstract

“…Dissimilarly, greenish dwarfing embryo, splenomegaly and cerebral edema for variant IBDV were recorded by Amer et al, (2007). The pathogenicity test with IBDV resulted in a significant decrease (p>0.05) in RWBF and histopathological lesion score in comparison with c-ve one at 5, 10 d PI that came in accordance with Kurukulasuriya et al , (2017).…”

Section: A B Csupporting

confidence: 84%

Epidemiological study on Infectious bursal disease in broiler chicken farms in some Governorates in Delta Egypt

El-Shorbagy

Saad

Zyan

et al. 2018

Benha Veterinary Medical Journal

View full text Add to dashboard Cite

This study was conducted to estimate the prevalence of IBD in broiler farms from different localities in different Governorates; AlQalubia, AlGharbia, AlMenofia and ALBehera and also to make a further characterization of the obtained strains through nucleotide sequencing then evaluate the pathogenicity of the strain in SPF ECE and commercial broiler chicks. Fifteen out of a total of 32 (46.87%) bursal samples showed 620 bp amplicon size of HVR of VP2 when tested with RT-PCR. The nucleotide sequencing classified two strains as vvIBDV and one was similar to classical attenuated vaccines. The vvIBDV strain resulted in 100 % mortality of inoculated embryos with moderate to severe congestion of the body, cranial hemorrhage, congested and greenish liver with necrotic foci. The CAMs showed thickening with petechial hemorrhage. The experimental infection with (IBDV_ EGY 2018/N23) isolate was assessed in vaccinated groups with hot, intermediate and hot + intermediate vaccines and unvaccinated 25 days old Cobb broiler chicks; the c+ve group showed a significant difference in RWBF and cumulative bursal lesion in comparison with c-ve group. It recorded higher MSI than c-ve. All vaccinated groups showed a significant increase in Abs titer when compared with non-vaccinated one and the hot vac. Group recorded the highest titer. They also showed a non-significant difference in RWBF and cumulative bursal lesion score in comparison to c-ve one at ten dPI and lower MSI than c+ve but the hot + intermediate vac. group recorded the lowest lesion score and MSI. This study concluded the reemergence and circulation of vvIBDV despite the intensive vaccination strategies.

show abstract

“…The 20 of the investigated flocks showed clinical manifestations of depression, dehydration, and whitish diarrhoea, and mortality ranged from 15-20%. These findings are in concur with [4,36]. In addition, the predominant necropsy findings included lesions of the bursa of Fabricius: atrophy, enlargement, oedema, hemorrhages, and congestion with petechial and ecchymotic hemorrhages in the pectoral muscles.…”

Section: Clinical Signs Mortalities and Pm Lesionssupporting

confidence: 79%

The Potential Diversity of Intestinal Enterobacteriaceae in Broiler Chickens is Associated with Infectious Bursal Disease Virus Infection

Mosa,

Salem,

Bastamy

et al. 2024

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

HE aim of this work is to study the Enterobacteriaceae-related bacteria of the gut microbiota with infectious bursal disease virus infection (IBDV) in naturally and experimentally infected broiler chickens. From the field, samples were collected from 20 suspected Gumboro-infected and 5 apparently healthy farms. For the experimental study, 36-day-old chicks were allocated to 2 groups of 18 birds each, then at 14 days old, G1 was challenged via eye drop with IBDV strain MK088026, and G2 was kept as control negative. RT-PCR revealed that 75% of farms were IBDpositive. The aerobic bacterial counts in positive farms were significantly higher than in apparently healthy individuals. The bacterial counts on MacConkey, IBD-positive farms were significantly higher than apparently healthy. Also, positive farms showed significantly higher lactose and nonlactose fermenter counts than apparently healthy ones. Serologically, the bacterial isolates from positive farms were 5 serotypes of E. coli identified as E. coli O78, O127H6, O91H21, O159, and O1H7. On apparently healthy, 3 E. coli serotypes were recorded as O128 H2, O146H21, and O2H6. Also, in positive farms, the non-lactose fermenter was Proteus mirabillus, Provedencia rettgeri, Salmonella kentacy. and Salmonella typhemurium while, in apparently healthy, 3 isolates were Salmonella entritedis, Salmonella larochella, and Salmonella typhemurium. On the experimental level, the bacterial counts on different media in challenged birds were higher than control. The serologically identified bacteria in G1 were 3 E. coli serotypes (O26H11, O78, and O128H2) while in G2, 3 E. coli (O26H11, O78, and O55H7). Our results indicated that IBDV infection was associated with an increased number of Enterobacteriaceae-related bacteria in the chicken gut.

show abstract

Isolation, molecular characterization and pathogenicity studies of infectious bursal disease field virus isolates

Cited by 3 publications

References 48 publications

The Effectiveness of Immunostimulants on NDV Genotype VII and IBDV Infection in Broiler Chickens

The Effectiveness of Immunostimulants on NDV Genotype VII and IBDV Infection in Broiler Chickens

Epidemiological study on Infectious bursal disease in broiler chicken farms in some Governorates in Delta Egypt

The Potential Diversity of Intestinal Enterobacteriaceae in Broiler Chickens is Associated with Infectious Bursal Disease Virus Infection

Contact Info

Product

Resources

About