Isolation, molecular characterization and antimicrobial resistance of enterobacteriaceae isolated from fish and seafood

Dib, Amira Leila; Almasi‐Hashiani, Amir; Chahed, Amina; Kürekçi, Cemil; Moreno, Elena; Espigares, M.; Espigares, Elena

doi:10.1016/j.foodcont.2018.01.005

Cited by 53 publications

(36 citation statements)

References 44 publications

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“…The occurrence of ESBL-producing Escherichia coli was low in Nile perch and water samples collected at offshore fishing grounds and at landing sites of Lake Victoria but higher in fish from local fish markets in Mwanza. ESBL-producing E. coli have been reported in different wild-caught fish species in Switzerland, Algeria, and India (Abgottspon et al, 2014;Singh et al, 2017;Dib et al, 2018), as well as in farmed fish in China (Jiang et al, 2012) and Egypt (Ishida et al, 2010). Moremi et al (2016b) reported a slightly higher prevalence of ESBL-producing bacteria in wildcaught tilapia sold at markets in Mwanza (13.3%) as compared with our findings in Nile perch (8.3%).…”

Section: Discussionsupporting

confidence: 64%

“…The SHV enzyme seems more dominant in Klebsiella pneumoniae among the Enterobacteriaceae (Rupp and Fey, 2003). The CTX-M enzymes are often the most common ESBLs produced by E. coli and have been isolated in human clinical, animal food, and environmental samples (Egea et al, 2012;Boonyasiri et al, 2014;Dib et al, 2018). On the other hand, the TEM enzymes especially of TEM-1 and TEM-2 are predominantly reported in E. coli and other members of Enterobacteriaceae, although TEM-1 is not considered as ESBLs.…”

Section: Introductionmentioning

confidence: 99%

“…The reservoirs of ESBL-producing E. coli are warm-blooded animals including humans, which can transmit the bacteria to different environments, for example, aquatic environments, through fecal pollution (Jiang et al, 2012). Recently, studies have reported ESBL-producing E. coli in seafood such as shrimps, sardines, farmed fish, Nile tilapia (Oreochromis niloticus) as well as in frozen mackerel (Jiang et al, 2012;Moremi et al, 2016b;Nasreldin and Khaldoon, 2015;Dib et al, 2018). It has been documented that urban sewage, for example, from hospitals, is an important source of ESBL-producing enteric bacteria (Ojer-Usoz et al, 2013;Abgottspon et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Genome-Based Analysis of Extended-Spectrum β-Lactamase-Producing Escherichia coli in the Aquatic Environment and Nile Perch (Lates niloticus) of Lake Victoria, Tanzania

et al. 2020

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Extended-spectrum β-lactamase (ESBL)-producing bacteria constitute an emerging global health issue with food products being vehicles of transmission and the aquatic environments serving as potential reservoirs. This study aimed to characterize ESBLproducing Escherichia coli in Nile perch and water from Lake Victoria in Tanzania. A total of 180 samples of Nile perch and 60 water samples were screened for ESBL-producing E. coli on MacConkey agar supplemented with 2 µg/ml of cefotaxime and confirmed by bla CTX−M and bla TEM PCR. Antimicrobial resistance was determined by the disk diffusion method, and the ESBL-producing isolates were whole genome sequencing (WGS). ESBL-producing E. coli were detected in eight of the 180 analyzed Nile perch samples, and only one water sample was positive (1.7%, n = 60). Isolates were resistant to sulfamethoxazole-trimethoprim (100%), ampicillin/cloxacillin (100%), erythromycin 72.7% (8/11), tetracycline 90.9% (10/11), and nalidixic acid 63.6% (7/11). This mostly corroborates the resistance genes that they carried for sulfonamides (sul1 and sul2), trimethoprim (dfrA and dfrB), aminoglycosides [aac(3)-IId, strA, and strB], tetracycline [tet(B) and tet(D)], and fluoroquinolones (qepA4). They harbored plasmid replicon types IncF, IncX, IncQ, and Col and carried bla CTX−M−15 and bla TEM−1B genes generally found on the same contigs as the IncF plasmid replicon. Although epidemiologically unrelated, the strains formed three separate sequence type-phylogroup-serotypespecific clusters: C1, C2, and C3. Cluster C1 included five strains (3 to 13 SNPs) belonging to ST167, phylogroup A, and serotype O9:H21; the two C2 strains (11 SNPs) belong to ST156, phylogroup B1, and serotype ONT:H28; and C3 was made up of four strains (SNPs ranged from 4 to 17) of ST636, phylogroup B2, and serotype O45:H7. The common virulence gene gad was reported in all strains. In addition, strains in C2 and C3 possessed iss, lpfA, and nfaE virulence genes, and the vat gene was found only in C3. The present study reports the occurrence of multidrug-resistant ESBL-producing Frontiers in Microbiology | www.frontiersin.org February 2020 | Volume 11 | Article 108 Baniga et al.Genomics of ESBL-E. coli in Lake Victoria E. coli carrying plasmid-mediated ESBL genes in offshore water and Nile perch in Lake Victoria. Strains formed three clonal clusters of unknown origin. This study reveals that the Lake may serve as reservoir for ESBL-producing bacteria that can be transmitted by fish as a food chain hazard of One-Health concern.

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Section: Discussionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Based Analysis of Extended-Spectrum β-Lactamase-Producing Escherichia coli in the Aquatic Environment and Nile Perch (Lates niloticus) of Lake Victoria, Tanzania

et al. 2020

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“…Muitas bactérias produtoras de genes de resistência são encontradas em ambiente hospitalar, em diversos reservatórios ambientais (BLAAK et al, 2014;FRANZ et al, 2015), alimentos (THORSTEINSDOTTIR et al, 2010HUSSAIN et al, 2017), peixes e frutos do mar (DIB et al, 2018).…”

Section: Resultsunclassified

Caracterização Físico-Química, Antioxidante E De Aminoácidos Da Castanha Do Baru, Castanha De Caju E Castanha-Do-Brasil

Barboza¹,

Pereira²,

Fuzinatto³

et al. 2019

Qualidade De Produtos De Origem Animal 2

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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“…Also, seafood-associated foodborne pathogen outbreaks are concern. Most of them are commonly contaminated with several pathogenic microorganisms such as Vibrio parahaemolyticus, Escherichia coli, Salmonella, and Listeria monocytogenes; however, others such as Clostridium botulinum and Aeromonas hydrophila are also associated with marine food products [52,53]. Consumption of the contaminated seafood with pathogenic microorganisms can lead to foodborne illnesses in the form of infection, intoxication, or both [53].…”

Section: Seafood-based Productsmentioning

confidence: 99%

Edible Films Incorporated with Active Compounds: Their Properties and Application

Rawdkuen¹

2019

Active Antimicrobial Food Packaging

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Antimicrobial compounds are food additives, which play a major role to reduce food spoilage. There are three main groups of antimicrobial compounds such as chemical agent, natural extract, and probiotics. The direct incorporations of the active compounds on the surface of food may have limited benefit because they are rapidly diffused from the food surface into the food product, resulting in the limited efficacy of these compounds. Thus, incorporation of antimicrobial compounds into packaging matrix, especially biopolymer film is a promising technique to reduce contaminations and inhibit, retard, and/or kill the microorganisms. Edible films are thin layer of natural polymers used to maintain the physicochemical quality of foods and extend their shelf life. A variety of biopolymeric-based materials including polysaccharides, proteins, and lipids have been extensively used for antimicrobial packaging and can be used as a carrier of active compounds. Incorporation of antimicrobial compounds may or may not enhance the mechanical properties and water vapor permeability of biopolymer films. The applications of active films can reduce contamination through the releasing of antimicrobial compound, thus reducing the risk from pathogen, extending shelf life of the packaged foods, and providing better quality with high safety.

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Isolation, molecular characterization and antimicrobial resistance of enterobacteriaceae isolated from fish and seafood

Cited by 53 publications

References 44 publications

Genome-Based Analysis of Extended-Spectrum β-Lactamase-Producing Escherichia coli in the Aquatic Environment and Nile Perch (Lates niloticus) of Lake Victoria, Tanzania

Genome-Based Analysis of Extended-Spectrum β-Lactamase-Producing Escherichia coli in the Aquatic Environment and Nile Perch (Lates niloticus) of Lake Victoria, Tanzania

Caracterização Físico-Química, Antioxidante E De Aminoácidos Da Castanha Do Baru, Castanha De Caju E Castanha-Do-Brasil

Edible Films Incorporated with Active Compounds: Their Properties and Application

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