Isolation, molecular and phenotypic characterization, and antibiotic susceptibility of Cronobacter spp. from Brazilian retail foods

Brandão, Marcelo Luiz Lima; Umeda, Natália Scudeller; Jackson, Emily E.; Forsythe, Stephen; Filippis, Ivano de

doi:10.1016/j.fm.2016.11.011

Cited by 60 publications

(63 citation statements)

References 44 publications

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“…The C. malonaticus ST440 strain was susceptible to the 13 antibiotics tested. This result was similar to those obtained by other authors who reported that Cronobacter strains isolated from food and water samples were susceptible to almost all the antibiotics tested (Brandao et al ; Fei et al ; Vasconcellos et al ).…”

Section: Resultssupporting

confidence: 92%

“…Cronobacter has been isolated from different categories of retail foods in Brazil (Brandao et al ; Vasconcellos et al ), but no research has been undertaken on drinking water. Ayaz et al () evaluated the cleaning practices and contamination status of contents and teats of feeding bottles used by children admitted in a hospitals in Pakistan and observed that 5·6% of the mothers/caretakers use natural mineral waters to prepare feeding bottle for children.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of total coliforms,Escherichia coliandCronobacterspecies in commercially available 20 l bottled drinking water sold in Rio de Janeiro State, Brazil

Vasconcellos¹,

Medeiros²,

Rosas³

et al. 2019

Lett Appl Microbiol

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Cronobacter infections of infants are commonly regarded as due to the ingestion of contaminated feed. The aim of this study was to determine the occurrence of Cronobacter, total coliforms and Escherichia coli in different brands of natural mineral waters as sold in 20 l returnable bottles in Rio de Janeiro, Brazil. The quantification of total coliforms and E. coli was performed by Most Probable Number. The detection of Cronobacter was as according to the ISO 22964:2017 and Bacteriological Analytical Manual/FDA. Molecular characterization of Cronobacter isolates was performed by real‐time PCR and by multi‐locus sequence typing. The antibiotic susceptibility profile was determined and biofilm production was evaluated in polystyrene microplates. Total coliforms and E. coli were detected in 13 (39·4%) and 2 (6·1%) of the 33 lots analysed respectively, and were considered unsatisfactory for human consumption according to Brazilian law. One (3·0%) lot showed contamination by C. malonaticus ST440 (Cronobacter MLST Databases accession no. ID 2646). The strain was susceptible to all (n = 13) antibiotics tested and only formed a weak biofilm. Since there is a high consumption of natural mineral waters by elderly and immunosuppressed persons, epidemiological surveillance agencies should be aware of the risk that these waters may represent for these groups. Significance and Impact of the Study Cronobacter malonaticus ST440 was isolated from 20 l bottled drinking natural mineral waters sold in markets in Rio de Janeiro State, Brazil, and can be a potential threat to human health, particularly for neonates. Thirteen lots (39·4%) were unsatisfactory for human consumption due to the presence of total coliforms and/or Escherichia coli.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Occurrence of total coliforms,Escherichia coliandCronobacterspecies in commercially available 20 l bottled drinking water sold in Rio de Janeiro State, Brazil

Vasconcellos¹,

Medeiros²,

Rosas³

et al. 2019

Lett Appl Microbiol

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“…Notably, these organisms can cause rare but life-threatening diseases in neonates and immunocompromised infants, including meningitis, necrotizing enterocolitis, and septicemia (4,5). The bacterium has been isolated from the environment, food, and clinical sources (2,(6)(7)(8)(9)(10)(11)(12)(13). To date, three species, C. sakazakii, C. malonaticus, and C. turicensis, have been reported to cause clinical infection (14).…”

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confidence: 99%

Reconstituting the History of Cronobacter Evolution Driven by Differentiated CRISPR Activity

Zeng¹,

Zhang²,

Wu³

et al. 2018

Appl Environ Microbiol

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strains harboring the CRISPR-Cas system are important foodborne pathogens causing serious neonatal infections. However, the specific role of the CRISPR-Cas system in bacterial evolution remains relatively unexplored. In this study, we investigated the impact of CRISPR-Cas in evolution and obtained 137 new whole-genome sequences of by next-generation sequencing technology. Among the strains examined (n=240), 90.6% (193/213) of prevalent species ,, and strains had intact CRISPR-Cas systems. Two rare species, (n=2) and (n=6), lacked and preserved the CRISPR-Cas system at a low frequency (1/6), respectively. These results suggest that the presence of one CRISPR-Cas system in is important for the species to maintain genome homeostasis for survival. The ancestral strain was likely to harbored both subtype I-E and I-F CRISPR-Cas systems, during the long evolutionary process, subtype I-E was retained, while subtype I-F selectively degenerated in species and was even lost in the major pathovars. Moreover, significantly higher CRISPR activity was observed in plant-associated species than in the virulence-related species and Similar spacers of CRISPR arrays were rarely found among species, suggesting intensive change through adaptive acquisition and loss. Differentiated CRISPR activity appears to be the product of environmental selective pressure and might contribute to the bidirectional divergence and speciation of This study reports the evolutionary history of under the selective pressure of the CRISPR-Cas system. One CRISPR-Cas system in is important for maintaining genome homeostasis, whereas two types of systems may be redundant and not conducive for acquiring beneficial DNA for environmental adaption and pathogenicity. Differentiated CRISPR activity has contributed to the bidirectional divergence and genetic diversity of This perspective makes a significant contribution to the literature by providing new insights into CRISPR-Cas systems in general, while further expanding the roles of CRISPR beyond conferring adaptive immunity and demonstrating a link to adaptation and species divergence in a genus. Moreover, our study provides new insights into the balance between genome homeostasis and the uptake of beneficial DNA related to CRISPR-based activity in the evolution of .

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“…from foods, using conventional microbiological and molecular techniques worldwide. These surveillance studies include research on raw dried pasta from the German market [42], wheat flour from China [43], dehydrated rice powder from the Chinese Supermarket [44], dried food from Japan [45], retails foods from Brazil and Czech Republic [11,46], ready-to-eat foods other than infant formula from Ireland and Switzerland [47], ready-to-eat foods from China [48], herbs and spices from Jordon [49], medicinal plants, herbs, and spices from India [50], spices and herbs from Poland [51], infant formula production factory premises and powdered infant formula from China [52]. The molecular tools were also successfully used in the reevaluation of a suspected Cronobacter sakazakii outbreak in Mexico [53].…”

Section: Discussionmentioning

confidence: 99%

Molecular Surveillance of Cronobacter spp. Isolated from a Wide Variety of Foods from 44 Different Countries by Sequence Typing of 16S rRNA, rpoB and O-Antigen Genes

Miranda

Banerjee

Simpson

et al. 2017

Foods

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Cronobacter spp. are emerging infectious bacteria that can cause acute meningitis and necrotizing enterocolitis in neonatal and immunocompromised individuals. Although this opportunistic human-pathogenic microorganism has been isolated from a wide variety of food and environmental samples, it has been primarily linked to foodborne outbreaks associated with powdered infant formula. The U.S. Food and Drug Administration use the presence of these microbes as one of the criteria to assess food adulteration and to implement regulatory actions. In this study, we have examined 195 aliquots of enrichments from the nine major categories of foods (including baby and medical food, dairy products, dried food, frozen food, pet food, produce, ready-to-eat snacks, seafood, and spices) from 44 countries using conventional microbiological and molecular techniques. The typical colonies of Cronobacter were then identified by VITEK2 and real-time PCR. Subsequently, sequence typing was performed on the 51 recovered Cronobacter isolates at the 16S rRNA, rpoB and seven O-antigen loci for species identification in order to accomplish an effective surveillance program for the control and prevention of foodborne illnesses.

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Isolation, molecular and phenotypic characterization, and antibiotic susceptibility of Cronobacter spp. from Brazilian retail foods

Cited by 60 publications

References 44 publications

Occurrence of total coliforms,Escherichia coliandCronobacterspecies in commercially available 20 l bottled drinking water sold in Rio de Janeiro State, Brazil

Occurrence of total coliforms,Escherichia coliandCronobacterspecies in commercially available 20 l bottled drinking water sold in Rio de Janeiro State, Brazil

Reconstituting the History of Cronobacter Evolution Driven by Differentiated CRISPR Activity

Molecular Surveillance of Cronobacter spp. Isolated from a Wide Variety of Foods from 44 Different Countries by Sequence Typing of 16S rRNA, rpoB and O-Antigen Genes

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