Isolation, LC–MS/MS and 2D-NMR characterization of alkaline degradants of tenofovir disoproxil fumarate

Anandgaonkar, Vaibhav; Gupta, Abhishek; Kona, Srinivas; Talluri, M.V.N. Kumar

doi:10.1016/j.jpba.2014.12.034

Cited by 14 publications

(4 citation statements)

References 13 publications

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“…al. 7 as degradation products of TDF which in fact should only be considered as interaction products of drug base with the solvent.…”

Section: Postulated Degradation Pathway For Non-volatile Degradation ...mentioning

confidence: 99%

“…6 Recently, a study reported two alkaline degradation products of TDF, viz., methyl hydrogen ({[1-(6-amino-9H-purin-9-yl)-propan-2-yl]oxy}methyl) phosphonate and dimethyl({[1-(6-amino-9Hpurin-9-yl)propan-2-yl]oxy} methyl)phosphonate. 7 Some other studies are available on degradation behaviour of the drug, but these are limited to solution state, and structures of the degradation products were also not elucidated. [8][9][10][11][12][13] To best of our knowledge, no comprehensive report exists on solid as well as solution state degradation behaviour of TDF, which includes characterization of its all degradation products.…”

Section: Introductionmentioning

confidence: 99%

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Stability behaviour of antiretroviral drugs and their combinations. 1: characterization of tenofovir disoproxil fumarate degradation products by mass spectrometry

et al. 2015

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“…al. 7 as degradation products of TDF which in fact should only be considered as interaction products of drug base with the solvent.…”

Section: Postulated Degradation Pathway For Non-volatile Degradation ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stability behaviour of antiretroviral drugs and their combinations. 1: characterization of tenofovir disoproxil fumarate degradation products by mass spectrometry

et al. 2015

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“…A similar approach involving comparison of the measured formulae of the eight unknown constituents and their product ions with those of compounds with similar skeletons could be used to deduce their structures. However, validation using further structural analysis methods such as NMR spectroscopy would be required 32 . The results indicated that the developed method can provide support to better study the pharmacodynamic basis of Salviae miltiorrhizae.…”

Section: Resultsmentioning

confidence: 99%

A simplified strategy for molecular formula determination of chemical constituents in traditional Chinese medicines based on accurate mass, A + 1 and A + 2 isotopic peaks using Fourier transform ion cyclotron resonance mass spectrometry

Zhang

Wang

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Recently, isotopic fine structures derived from Fourier transform ion cyclotron resonance mass spectrometry have been used to determine the molecular formula for unknown compounds in many complex systems. However, a simplified strategy for molecular formula determination of chemical constituents in traditional Chinese medicines (TCMs) based on accurate mass, A + 1 and A + 2 isotopic peaks is necessary. Methods Salviae miltiorrhizae was selected as a representative species. First, the chemical constituents were chromatographically separated and their accurate masses were obtained. The A + 1 and A + 2 isotopic peaks of all chemical constituents were then also acquired. Finally, the chemical formulae of the chemical constituents were determined. Results In the sample of Salviae miltiorrhizae, the formulae of 38 CHO‐containing chemical constituents were quickly determined, and all chemical constituents were identified using their tandem mass spectrometric data. Moreover, the method was validated by comparison of the A + 1 and A + 2 isotopic peaks, their fragmentation patterns and the retention times of six selected standard substances. Conclusions The results demonstrate that the described strategy performs well for molecular formula determination of chemical constituents in TCMs. This also indicates that this method will be meaningful for the structural identification of chemical constituents of TCMs.

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“…Different separative techniques, such as HPLC–UV [12–17], LC–MS [18,19], and high‐performance thin‐layer chromatography [20], have been introduced for the analysis of antiretroviral drugs.…”

Section: Introductionmentioning

confidence: 99%

Determination of antiretroviral drugs for buyers’ club in Switzerland using capillary electrophoresis methods

et al. 2020

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Human immunodeficiency virus‐acquired immunodeficiency syndrome continues to be a major global public health issue, having claimed almost 33 million lives to date. Due to the high cost of antiretroviral treatment, access to these drugs remains difficult for vulnerable populations, such as migrants and people living in prisons, who often do not have health insurance. These factors lead to poorer health outcomes and higher transmission rates. The personal importation scheme for unapproved generics from foreign countries is one option to access affordable human immunodeficiency virus treatment. However, the risk of importing falsified medicine remains high, and the quality control of unapproved drugs is lacking. In this context, three CE methods for the analysis of nine antiviral drugs found in commercial pharmaceutical formulations were evaluated. The selected compounds were emtricitabine, tenofovir disoproxil, tenofovir alafenamide, rilpivirine, efavirenz, raltegravir, dolutegravir, abacavir, and lamivudine. The developed methods were successfully applied to determine the active pharmaceutical ingredients of commercial formulations and unapproved generics. The quality control of unapproved generics by CE is an attractive approach due to its good standard of quality, low cost, ecofriendliness, and ease of implementation.

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Isolation, LC–MS/MS and 2D-NMR characterization of alkaline degradants of tenofovir disoproxil fumarate

Cited by 14 publications

References 13 publications

Stability behaviour of antiretroviral drugs and their combinations. 1: characterization of tenofovir disoproxil fumarate degradation products by mass spectrometry

Stability behaviour of antiretroviral drugs and their combinations. 1: characterization of tenofovir disoproxil fumarate degradation products by mass spectrometry

A simplified strategy for molecular formula determination of chemical constituents in traditional Chinese medicines based on accurate mass, A + 1 and A + 2 isotopic peaks using Fourier transform ion cyclotron resonance mass spectrometry

Determination of antiretroviral drugs for buyers’ club in Switzerland using capillary electrophoresis methods

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