Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues

Abstract

Lung-derived mesenchymal stem cells (LMSCs) are considered to be important in lung tissue repair and regenerative processes. However, the biological characteristics and differentiation potential of LMSCs remain to be elucidated. In the present study, fetal lung-derived mesenchymal stem cells (FLMSCs) were isolated from fetal bovine lung tissues by collagenase digestion. The in vitro culture conditions were optimized and stabilized and the self-renewal ability and differentiation potential were evaluated. The results demonstrated that the FLMSCs were morphologically consistent with fibroblasts, were able to be cultured and passaged for at least 33 passages and the cell morphology and proliferative ability were stable during the first 10 passages. In addition, FLMSCs were found to express CD29, CD44, CD73 and CD166, however, they did not express hematopoietic cell specific markers, including CD34, CD45 and BOLA-DRα. The growth kinetics of FLMSCs consisted of a lag phase, a logarithmic phase and a plateau phase, and as the passages increased, the proliferative ability of cells gradually decreased. The majority of FLMSCs were in G0/G1 phase. Following osteogenic induction, FLMSCs were positive for the expression of osteopontin and collagen type I α2. Following neurogenic differentiation, the cells were morphologically consistent with neuronal cells and positive for microtubule-associated protein 2 and nestin expression. It was concluded that the isolated FLMSCs exhibited typical characteristics of mesenchymal stem cells and that the culture conditions were suitable for their proliferation and the maintenance of stemness. The present study illustrated the potential application of lung tissue as an adult stem cell source for regenerative therapies.

Multipotent mesenchymal stem cells in lung fibrosis

“…As generally accepted, MSC are self-renewing cells characterized by i) plastic adherence, ii) expression of CD44, CD73, CD90 and CD105, iii) lack of expression of hematopoietic markers, and iv) their ability to differentiate into multiple cell types such as adipocytes, osteocytes and chondrocytes [ 24 ]. In accordance with previous reports for the characterization of MSC [ 25 , 26 ], the cells of interest isolated from the lung stained positive for the mesenchymal markers CD44, CD90, CD105. Additionally, the cells had the capacity to differentiate into adipocytes, osteocytes and chondrocytes, identifying them as MSC.…”

Application of antibody-conjugated small intestine submucosa to capture urine-derived stem cells for bladder repair in a rabbit model

“…Also known as Integrin beta 1, an adhesion molecule, the CD29 can bind with a variety of ligands and play an important role in the mobilization, homing, migration and differentiation of stem cells as well as mediation of niche interactions [ [72] , [73] , [74] , [75] , [76] , [77] ]. As a surface marker for the MSCs, CD29 is essential for cell adhesion and recognition in a variety of biological processes including embryogenesis, tissue repair and immune response [ 78 , 79 ]. Since no single specific marker has been identified for the USC, we have chosen CD29 as a distinctive target for the capture of the USCs in this study.…”

Nestin-Expressing Cells in the Lung: The Bad and the Good Parts

“…The neural cells were positive for myelin basic protein, GFAP, and nestin. Similarly, Hu et al [ 12 ] isolated cells from fetal bovine lung tissues that were morphologically consistent with fibroblasts, expressed surface markers of MSCs, and were induced to undergo osteogenic and neurogenic differentiation. Neuronal cells were positive for microtubule-associated protein 2 and nestin expression.…”