Isolation, Identification of Lactobacillus mucosae AN1 and its Antilisterial Peptide Purification and Characterization

Ayyanna, Repally; Venkatesh, Perumal; Ankaiah, Dasari; Esakkiraj, Palanichamy; Arul, Venkatesan

doi:10.1007/s12602-017-9341-3

Cited by 19 publications

(13 citation statements)

References 42 publications

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“…Similar studies showed that Pediococcus pentosaceus CE65 able to survive at 0.3% bile salts (Manini et al, 2016). Another study with L. mucosae AN1 also showed resistance towards bile salts from 0.1 to 0.7% (Repally et al, 2018).…”

Section: Discussionmentioning

confidence: 53%

Isolation and characterization of Lactobacillus brevis C23 with ability to secrete antimicrobial substance for the inhibition of a foodborne pathogen Listeria monocytogenes ATCC 7644

Sreedharan¹,

Abbasiliasi²,

Murugan³

et al. 2021

MJM

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This study aims to isolate lactic acid bacteria (LAB) from various food sources to obtain a potent strain against Listeria monocytogenes. Methodology and results: A total of 68 LAB isolates were selected to evaluate their antimicrobial activity against L. monocytogenes, a foodborne pathogen and a causative agent of listeriosis. The selected isolate was identified and characterized. The isolate C23 from cabbage showed the highest antimicrobial activity against L. monocytogenes ATCC 7644 with inhibition ability of 73.94%. The isolate was closely related to Lactobacillus brevis by 16S rRNA sequencing and subsequently deposited in GenBank with an accession number of MN880215, named as L. brevis C23. The cell free supernatant (CFS) of L. brevis C23 had high tolerance in low pH and was able to withstand up to 60 °C. The proteinaceous nature of the antimicrobial agent was also confirmed through the enzymatic test. The CFS was stable on different detergents as well as bile salts. Under transmission electron microscopy (TEM), the inhibitory effect of CFS against L. monocytogenes was proven by causing cell lysis. Conclusion, significance and impact of study: Bacteriocin-like inhibitory substances (BLIS) of L. brevis C23 showed very promising potential in food industrial application.

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Section: Discussionmentioning

confidence: 53%

Isolation and characterization of Lactobacillus brevis C23 with ability to secrete antimicrobial substance for the inhibition of a foodborne pathogen Listeria monocytogenes ATCC 7644

Sreedharan¹,

Abbasiliasi²,

Murugan³

et al. 2021

MJM

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“…Probiotic bacteria Lactobacillus mucosae (GenBank, KM068788), Lactobacillus fermentum (GenBank, KM068787) were isolated from sheep milk and fermented rice gruel, respectively (Repally et al, 2017). These probiotic strains were used for the treatment of inflammation induced in hind rats paw tissues.…”

Section: Methodsmentioning

confidence: 99%

Anti-inflammatory and Antioxidant Properties of Probiotic Bacterium Lactobacillus mucosae AN1 and Lactobacillus fermentum SNR1 in Wistar Albino Rats

2018

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The potent antioxidant probiotic strains Lactobacillus mucosae AN1 and Lactobacillus fermentum SNR1 were assessed for anti-inflammatory properties in carrageenan (acute) and complete Freund’s adjuvant-induced inflammation (chronic) models in the present study. The two probiotic strains were administered orally along with feed to the Wistar albino male rats as whole cell as well as microencapsulated form. The following experiments were performed to evaluate the anti-inflammatory properties of probiotic strains and the results were observed that the encapsulated and unencapsulated probiotic strains have exhibited statistically significant decrease in paw thickness. Percentage of inhibition in paw thickness of microencapsulated probiotic bacteria (Group VIII), unencapsulated strains (Group IX) were revealed 85 ± 13% and 77 ± 25%, respectively. In Hematoxylin and Eosin staining, results were revealed that the probiotic strains were exhibited anti-inflammatory effects on inflammation-induced paw tissues. qRT-PCR studies revealed upregulation of anti-inflammatory cytokine genes and down-regulation pro-inflammatory cytokine genes in probiotic-treated rat paw tissues. Further, the expression of anti-inflammatory and pro-inflammatory cytokines were examined using immunohistochemistry and ELISA methods. The probiotic administered rat paw tissue in different groups have exhibited the low level of lipid peroxides formation and higher anti-oxidant activities when compared to the control and inflammation control tissues.

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“…The bacterial strains were isolated aseptically from the gut content of indigenous country chicken and South Indian fermented tomato pickle (homemade) by spreading samples over MRS agar plates (a selective media to obtain Gram positive LAB only) and incubated anaerobically at 37°C. Several bacterial colonies were observed on the MRS agar plates after 42 h of incubation and randomly picked colonies were subjected for antimicrobial activity test by well diffusion method according to Repally et al (2018). Bacterial colonies were grown in MRS broth overnight at 37°C in an incubator shaker.…”

Section: Isolation and Identification Of Bacterial Isolatesmentioning

confidence: 99%

“…Further, the selected bacterial isolates were identified by 16s DNA sequencing. Universal 16S rRNA primers were used to amplify the 16S rRNA genes and the PCR conditions were implemented according to Repally et al (2018). The identified strains were labeled as B. subtilis CS (MG694567), Bacillus tequilensis CL (MG694566) and, Weissella cibaria p3B (MH473733).…”

Section: Isolation and Identification Of Bacterial Isolatesmentioning

confidence: 99%

Probiotic characterization of bacterial strains from fermented South Indian tomato pickle and country chicken intestine having antioxidative and antiproliferative activities

et al. 2021

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Aim: The present study aims to evaluate the potential antioxidant and antiproliferative properties of probiotic bacterial isolates Weissella cibaria p3B, Bacillus subtilis CS, and Bacillus tequilensis CL, isolated from South Indian fermented tomato pickle (homemade) and gut content of indigenous country chicken. Methods and Results: The bacterial isolates exhibited antimicrobial activity against food-borne, human pathogenic bacteria, along with better survival under different bile and acidic conditions, hydrophobicity towards several hydrocarbons, and adherence to intestinal epithelial cells (INT-407 cells). Also, the intact cell (IC) mixture of the three species showed better DPPH, ABTS, and Fe 2+ chelating activity as compared to the individual IC or cell extract (CE) activity. Among the three bacterial species, W. cibaria p3B revealed maximum antiproliferative activity against HeLa and Caco-2 cancer cells, all of which were nontoxic to INT-407 cells. Apart from being non-hemolytic, the bacterial isolates did not display any necrotic inhibition in HeLa and Caco-2 cells. The cell free supernatant (CFS) of the three bacterial isolates were tested for the production of antimicrobial peptides or bacteriocins. It found that the CFS of bacterial isolates was stable at various temperature, pH and sensitive to proteolytic enzymes confirms protenoius in nature of the antimicrobil peptides or bacteriocins. Conclusion:The bacterial isolates showed promising antimicrobial, antioxidant as well as antiproliferative activities with better survival ability at different pH and bile concentrations. The three bacterial isolates were able to produce potential antimicrobial peptides or bacteriocins. Significance and Impact of the study: These results indicate better compatibility of our bacterial isolates against synthetic drugs to avoid adverse side effects and can be processed as dietary supplements against food and human pathogens. They can also provide antioxidative and antiproliferative benefits to humans and animals.

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Isolation, Identification of Lactobacillus mucosae AN1 and its Antilisterial Peptide Purification and Characterization

Cited by 19 publications

References 42 publications

Isolation and characterization of Lactobacillus brevis C23 with ability to secrete antimicrobial substance for the inhibition of a foodborne pathogen Listeria monocytogenes ATCC 7644

Isolation and characterization of Lactobacillus brevis C23 with ability to secrete antimicrobial substance for the inhibition of a foodborne pathogen Listeria monocytogenes ATCC 7644

Anti-inflammatory and Antioxidant Properties of Probiotic Bacterium Lactobacillus mucosae AN1 and Lactobacillus fermentum SNR1 in Wistar Albino Rats

Probiotic characterization of bacterial strains from fermented South Indian tomato pickle and country chicken intestine having antioxidative and antiproliferative activities

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