Isolation, Identification, Biochemical and Antibiotic Sensitivity Characterization of Rhizobium Strains from Vigna mungo (L) Hepper, Cicer arietinum L and Vigna radiata (L) R Wilczek in Muzaffarnagar, Uttar Pradesh, India

Differential traits of rhizobia associated to root-nodules of gum acacia (Senegalia senegal), shittah tree (Vachellia seyal), pigeon pea (Cajanus cajan L) and cowpea (Vigna unguiculata)

“…Isolates were tested Gram negative and were microscopically rod-shaped and pink in colour after treatment with iodine reagent, confirming that they belonging to the genus Rhizobium. Related results were obtained respectively from Arachis hypogaea L. and Telfairia occidentalis in Nigeria (Agah et al, 2016), from Glycine max L. in Zambia (Kapembwa et al, 2016), from Mucuna pruriens L. in Nepal (Paudyal and Gupat, 2017), from V. mungo L. and V. radiata L. in India (Tyagi et al, 2017). These isolates were also recognized as acid producers, since they shifted the green colour of Bromothymol blue to yellow when incubated at 28±2°C for 3 days (Table 2).…”

Isolation and Biochemical Characterization of Indigenous Rhizobium Strains from Root Nodules of Field Grown Soybean Crop

“…All the top ten Rhizobium isolates showed positive test for glucose, lactose and sucrose fermentation. Similar results were reported by Sharma et al, (2009) and Tyagi et al (2017). Singh (2008) and Erum (2008) who also reported these sugar tests positive during isolation and characterization of Rhizobium.…”

“…The diversity and effectiveness of different isolates was studied on the basis of different biochemical tests viz. Triple sugar iron test, Starch utilization test and Catalase test (Tyagi et al, 2017). The isolates with an outstanding performance are identified, in a search for efficient and competitive strains for use in commercial inoculants in Chhattisgarh.…”

Isolation and characterization of Rhizobium spp. and Bradyrhizobium spp. from legume nodules

“…Starch hydrolysis, MR-VP test, and gelatin hydrolysis of 17 isolates were recorded negative results. This was consistent with Bergey manual for starch hydrolysis (Kuykendall, 2005;Kuykendall et al, 2005) but other studies showed different results (Panwar et al, 2012;Tyagi, Kumar, & Tomar, 2017). Although negative gelatin hydrolysis is a feature of Rhizobium spp.…”

“…For oxidase and catalase tests, there were both positive and negative results in rhizobial strains whereas these tests often got positive on Rhizobium spp. as reported (Deshwal & Chaubey, 2014;Gauri et al, 2011;Hossain et al, 2019;Panwar et al, 2012;Tyagi et al, 2017). The rest biochemical tests as urea hydrolysis, nitrate reduction, and citrate utilization varied in results depending on rhizobial species, as shown in Table 2, nevertheless, this illustrates diverse characteristics in rhizobia's metabolism even on the same Rhizobium species are highly affected by their living location (Becana & Sprent, 1987;Manhart & Wong, 1979;Panwar et al, 2012;Sadowsky et al, 1983;Streeter & DeVine, 1983;Thin et al, 2021).…”