Isolation, Identification, Antibacterial activity and Docking of Fatty acid and Fatty alcohol from Rumex dentatus Leaf Extract

Abstract

Rumex dentatus is a traditional medicinal plant that has been used in the treatment of anti-dermatitis, anti-inflammatory, antitumour, diarrhoea, eczema, constipation, and locally known Toothed dock. This article describes the isolation of compounds from CH3OH extract of Rumex dentatus leaf. The structures of the isolated compounds were identified by spectroscopic analysis, such as 1 H-NMR, 13 C-NMR, DEPT-135, COSY, HMBC, and HSQC. The extract and compounds were evaluated for antibacterial activity. The isolated compounds and antibacterial relationships were performed by molecular docking. The antibacterial activity was determined on Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa by agar well diffusion assay. Molecular docking studies were performed with the help of software such as Auto Dock Tools-1.5.6, Auto dock vina, chem3D pro 12.0.2.1076 and Discovery Studio Visualizer. The extract was showed maximum inhibition zone with Staphylococcus aureus, which indicates good antibacterial activity. The molecular docking was exhibited best results with DNA gyrase. The antibacterial and docking results were revealed that extract and compounds might be beneficially for antibacterial activities.

Isolation and Cytotoxicity Evaluation of Long Chain Bioactive Compounds from Commiphora swynnertonii (Burtt)

“…13 C NMR (150 MHz, d-CD2Cl2): δ (ppm) 14.45 (-CH3), 23.27, 25.30, 29.63, 29.82, 29.94, 30.04, 30.18, 30.27, 32.51 (each, -CH2), 34.09 (-CH2-C=O), and 177.87 (-C=O). The experimental spectral data above are in consistent with the literature data [20] . 6) as suggested by NIST library.…”

Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax

“…396.6899 equivalent to a fatty acid; hexacosanoic acid (C26H52O2). These spectral data were in agreement with Nyigo et al, 2022;Rehan et al, 2020;Yamamoto et al, 2015). Based to these spectral and literature information, compound G4 was assigned to be hexacosanoic acid (Figure 1) which is also known as cerotic acid.…”

An Overview on Rumex dentatus L.: Its Functions as a Source of Nutrient and Health-Promoting Plant

“…Ten more chemical constituents were identified and isolated from R. dentatus: isovanillic acid, succinic acid, gallic acid, p-hydroxycinnamic acid, quercetin, hexadecanoic acid 2, n-butyl-beta-D-fructopyranoside, 3dihydroxy propyl ester, daucosterol, helonioside A, and β-sitosterol. Furthermore, chlorogenic acid, vitamin C, vitamin A, p-hydroxybenzoic acid, cinnamic acid, syringic acid, ferulic acid, and vanillin benzoic acid which were also Evidence-Based Complementary and Alternative Medicine detected by HPLC, fatty acid, and momoterpenes (Figure 7) [16,[48][49][50].…”

“…is traditional Chinese medicine not only treats the Netherland dwarf fungal skin disease, but also has the functions of inhibiting bacteria and relieving itching, clearing away heat and toxic substances, strengthening the spleen and stomach, improving the immunity, and promoting the recovery of rabbit hair. More advantages include easy availability of components, scientific compatibility, simple manufacture, low cost, quick effect, no toxicity or side effects, no easy recurrence, and a high cure rate (>85%) [45,49,76]. 7.5.…”