Isolation, identification and vaccine development of serotype III Streptococcus parauberis in turbot (Scophthalmus maximus) in China

Gao, Ye; Liu, Yaokuan; Wang, Pengmei; Mo, Zhaolan; Li, Jie; Liu, Shulan; Li, Guiyang; Zhu, Ming

doi:10.1016/j.aquaculture.2021.736525

Cited by 12 publications

(12 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, M. marinum infections are only found in the southern areas with higher temperatures. Streptococcus parauberis infection was not detected in our previous 10-year investigation (Lan et al, 2020), and had not been reported to be isolated from fish in China until 2019 in turbots (Gao et al, 2021). In the current study, five cases of S. parauberis infection were detected, accounting for 3.23% of the total number of cases.…”

Section: Photobacterium Damselaecontrasting

confidence: 53%

“…At present, several pathogens of turbot have been reported in China, including Vibrio anguillarum (Zou et al, 2004), V. harveyi (Fan et al, 2005), V. alginolyticus (Zhang et al, 2006), Edwardsiella piscicida (Li et al, 2006), Aeromonas salmonicida (Lv et al, 2009), Mycobacterium marinum (Li et al, 2019), Streptococcus parauberis (Gao et al, 2021), iridovirus (Shi et al, 2005), and scuticociliate (Wang et al, 2005). The variety of pathogens increases the complexity of disease control in turbot farming.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Epidemiology of turbot bacterial diseases in China between October 2016 and December 2019

Gao

Wang²,

Liu³

et al. 2023

Front. Mar. Sci.

Self Cite

View full text Add to dashboard Cite

Turbot (Scophthalmus maximus) is an important commercial fish in China that can be infected by a series of bacterial pathogens, leading to great economic losses. In this study we focused on the epidemiology of turbot bacterial diseases in the major farming areas in China for three years. A total of 155 cases with 446 diseased turbots were investigated, and dominant bacterial pathogens were isolated from 137 cases (344 turbots). Thus, bacteria are the major threat to farming turbot in China. Edwardsiella piscicida was the major pathogen, which isolated as the dominant colony in 62 cases (40.00%) with 151 turbots (33.85%). Aeromonas salmonicida was isolated in 57 cases (36.77%) with 116 turbots (26.01%). Vibrio anguillarum was isolated in nine cases (5.81%), and Streptococcus parauberis in five cases (3.23%). Photobacterium damselae and Mycobacterium marinum were also isolated from one or two diseased fish. Other Vibrio spp. were isolated in 15 cases (9.68%). Two species of pathogen were isolated in 13 cases, and three species (E. piscicida, A. salmonicida, and S. parauberis) in one case. In 19 cases, no bacteria were isolated. Based on the annual disease analysis, we found that the E. piscicida infection proportion of total cases was greatly decreased, which may be caused by the attenuated vaccine inoculated in 2018. The antibiotic resistance of E. piscicida strains isolated in Weifang city was also determined. We found that the resistance to ceftriaxone, doxycycline, and SMZ/TMP were significantly increased from October 2016 to June 2018, and all the E. piscicida isolates exhibited resistance to SMZ/TMP in June 2018. These results indicated that E. piscicida is the major threat to turbot farming in China, and the attenuated E. piscicida vaccine exhibits effective protection. The usage of antibiotics may induce resistance quickly. Thus, development of vaccines is an important work for sustainable development of turbot farming in the future.

show abstract

Section: Photobacterium Damselaecontrasting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Epidemiology of turbot bacterial diseases in China between October 2016 and December 2019

Gao

Wang²,

Liu³

et al. 2023

Front. Mar. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Streptococcus parauberis could be classified into five serotypes which divided three subtypes of serotype I (Ia, Ib and Ic), serotype II, III, IV and V according to capsular polysaccharides gene structure (Gao et al, 2021; Yolanda et al, 2019; Torres‐Corral & Santos, 2020). Among these, serotypes Ia and Ib/Ic are found to be the most prevalent in streptococcosis in Paralichthys olivaceus , while serotype II does not induce mortality (Woo & Park, 2013).…”

Section: Introductionmentioning

confidence: 99%

Prevalence, antibiotic susceptibility and serotyping of Streptococcus parauberis isolates from diseased marine fish

et al. 2021

View full text Add to dashboard Cite

Streptococcus parauberis is the main causative bacterial pathogen for streptococcosis in marine fish, causing a significant economic loss to the mariculture industry. In this study, we aimed to investigate the serotypes and antimicrobial susceptibilities of 83 S. parauberis isolates from diseased farmed fish (Paralichthys olivaceus, Platichthys stellatus and Sebastes schlegelii) in Korea. This study categorized the S. parauberis isolates based on PCR serotyping into serotype I (subtypes Ia and Ib/Ic) and serotype II. Serotype Ia was identified to be significantly more prevalent than serotypes Ib/Ic and II. An antimicrobial susceptibility analysis showed that all of the isolates were susceptible to amoxicillin/clavulanic acid. Overall, the isolates studied showed resistance to penicillin and ampicillin (1.2%), tetracycline (68.7%), oxytetracycline (72.3%), doxycycline (53.0%) and erythromycin (37.3%). Interestingly, we found that serotypes Ia and II showed higher degrees of tetracycline resistance than serotype Ib/Ic. The macrolide resistance gene erm(B) was present in 75.8% of the Ia isolates, whereas the mef(A) gene was not detected in any of the isolates. The tetracycline resistance genes tet(S) and tet(M) were detected in 95.2% and 60% of Ia and II isolates respectively. Moreover, β‐lactam antibiotic‐resistant Ia isolates contained a set of 11 mutations in PBP1A and PBP2X. Our study provides a valuable reference for surveillance of antimicrobial resistance and underscores the strict management of antibiotic use to prevent the emergence of resistant of S. parauberis serotypes.

show abstract

“…Several potential virulence factors, including hyaluronic acid capsule, hyaluronidase, uberis factor, antiphagocytic factors (capsule, neutrophil toxin, M-like protein and R-like protein), plasminogen activator/streptokinase factor, surface dehydrogenase protein, CAMP factor, lactoferrin binding protein and surface adhesion molecule, have been identified in both S. uberis and S. parauberis in warm-blooded animals [ 98 , 99 , 100 ], but these factors have never been studied in strains isolated from diseased fish. Some strains of S. parauberis carrying capsuled polysaccharide genes have recovered from diseased fish [ 101 ], but the role of other virulent factors in the pathogenicity of these species needs further study.…”

Section: The Diseasementioning

confidence: 99%

“…Subsequently, the disease was reported in several species including turbot ( Scophthalmus maximus ), olive flounder, sea bass ( Sebastes ventricosus ), striped bass ( Morone saxatilis ) and ram cichlid ( Mikrogeophagus ramirezi ) [ 57 , 122 , 123 , 124 , 125 ]. More recently, S. parauberis classified as serotype III has been reported as the cause of streptococcosis in different turbot farms in China, and the isolates are different from those that infect flounder ( Paralichthys olivaceus ) in Japan and South Korea but similar to strains in Spain and the USA [ 101 ].…”

Section: The Diseasementioning

confidence: 99%

Streptococcosis a Re-Emerging Disease in Aquaculture: Significance and Phytotherapy

Soltani

Leitão

Shafiei

et al. 2022

Animals

View full text Add to dashboard Cite

Streptococcosis, particularly that caused by S. iniae and S. agalactiae, is a major re-emerging bacterial disease seriously affecting the global sustainability of aquaculture development. Despite a wide spread of the disease in aquaculture, few studies have been directed at assessing the in vitro antagonistic activity and in vivo efficacy of medicinal herbs and other plants against streptococcal agents. Most in vitro studies of plant extractives against S. iniae and S. agalactiae have found antibacterial activity, but essential oils, especially those containing eugenol, carvacrol or thymol, are more effective. Although essential oils have shown better anti-streptococcal activity in in vitro assays, in vivo bioassays require more attention. The extracts examined under in vivo conditions show moderate efficacy, increasing the survival rate of infected fish, probably through the enhancement of immunity before challenge bioassays. The available data, however, lack dosage optimization, toxicity and bioavailability assays of a specific plant or its bioactive compound in fish organs; hence, it is difficult to judge the validation of clinical efficacy for the prevention or treatment of fish streptococcosis. Despite the known bioactive compounds of many tested plants, few data are available on their mode of action towards streptococcal agents. This review addresses the efficacy of medicinal plants to fish streptococcosis and discusses the current gaps.

show abstract

Isolation, identification and vaccine development of serotype III Streptococcus parauberis in turbot (Scophthalmus maximus) in China

Cited by 12 publications

References 28 publications

Epidemiology of turbot bacterial diseases in China between October 2016 and December 2019

Epidemiology of turbot bacterial diseases in China between October 2016 and December 2019

Prevalence, antibiotic susceptibility and serotyping of Streptococcus parauberis isolates from diseased marine fish

Streptococcosis a Re-Emerging Disease in Aquaculture: Significance and Phytotherapy

Contact Info

Product

Resources

About