Isolation, identification, and structure-activity relationship of novel ACE inhibitory peptides from earthworm protein in vitro gastrointestinal digestion product

Lin, Zhengli; Wu, Hui; Zhang, Mengmeng

doi:10.1016/j.fbio.2023.103010

Cited by 4 publications

(2 citation statements)

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“…The distribution of molecular masses smaller than 3 kDa was analyzed by HPLC for different digestion processes. As shown in Figure 12, the percentage of peptides with a molecular weight below 1000 Da in the digested hydrolysate underwent a notable augmentation, mirroring the findings of Zhengli Lin's investigation [11]. The extent of reduction in the share of peptides exhibiting a molecular weight of over 2000 Daltons varied.…”

Section: Changes In Molecular Mass Distribution During Digestionsupporting

confidence: 65%

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Optimization of Extraction Process and Activity of Angiotensin-Converting Enzyme (ACE) Inhibitory Peptide from Walnut Meal

Meng,

She,

Feng

et al. 2024

Foods

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In order to further realize the resource reuse of walnut meal after oil extraction, walnut meal was used as raw material to prepare polypeptide, and its angiotensin-converting enzyme (ACE) inhibitory activity was investigated. The ACE inhibitory peptides were prepared from walnut meal protein by alkaline solution and acid precipitation. The hydrolysis degree and ACE inhibition rate were used as indexes to optimize the preparation process by single-factor experiment and response surface method. The components with the highest ACE activity were screened by ultrafiltration, and their antioxidant activities were evaluated in vitro. The effect of gastrointestinal digestion on the stability of walnut peptide was analyzed by measuring molecular weight and ACE inhibition rate. The results showed that the optimal extraction conditions were pH 9.10, hydrolysis temperature 54.50 °C, and hydrolysis time 136 min. The ACE inhibition rate of walnut meal hydrolysate (WMH) prepared under these conditions was 63.93% ± 0.43%. Under the above conditions, the fraction less than 3 kDa showed the highest ACE inhibitory activity among the ACE inhibitory peptides separated by ultrafiltration. The IC50 value of scavenging ·OH free radical was 1.156 mg/mL, the IC50 value of scavenging DPPH free radical was 0.25 mg/mL, and the IC50 value of scavenging O2− was 3.026 mg/mL, showing a strong total reducing ability. After simulated gastrointestinal digestion in vitro, the ACE inhibitory rate of walnut peptide decreased significantly, but it still maintained over 90% ACE inhibitory activity. This study provides a reference for the application of low-molecular-weight walnut peptide as a potential antioxidant and ACE inhibitor.

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Section: Changes In Molecular Mass Distribution During Digestionsupporting

confidence: 65%

“…The molecular mass distribution was analyzed by HPLC [11]. The enzyme solution of walnut meal was prepared into a 2 mg/mL solution and passed through a 0.45 µm filter membrane.…”

Section: Determination Of the Relative Molecular Mass Of Peptidesmentioning

confidence: 99%