Isolation, identification, and proteomic analysis of outer membrane vesicles of Riemerella anatipestifer SX-1

Wang, Ying; Deng, Jianfeng; Wang, Xiaorong; Zhang, Lixia; Xu, Yue; Ren, Jianle; Niu, Sheng; Zhao, Yujun; Yan, Fang; Tian, Wen-xia; Yan, Yi

doi:10.1016/j.psj.2024.103639

Cited by 1 publication

(2 citation statements)

References 38 publications

(40 reference statements)

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“…[51][52][53] CD8 + T cells, as fundamental antitumor effector cells, possess the capability to directly eliminate tumor cells and induce tumor regression. 30 Large-scale CD8 + T cells infiltration into tumor tissues reverses the immune-suppressed status of tumor and induces the transformation of cold tumor into hot ones, which helps to activate tumor immunity. 31 Bacterial-derived OMVs have been shown to activate the immune response and hold promise for application in antitumor immunotherapy.…”

Section: Discussionmentioning

confidence: 99%

“…A previously reported method was used to obtain Pd-OMVs from Pd cultures. 20,30 In brief, Parabacteroides distasonis, obtained from ATCC (ATCC 8503), was subcultured in BHI broth with 150 × g shaking at 37°C for 48 h under anaerobic conditions. The Pd cultures were then transferred to centrifuge tubes and centrifuged twice at 10,000 × g for 20 min at 4°C.…”

Section: Bacterial Culture and Pd-omvs Purificationmentioning

confidence: 99%

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Parabacteroides distasonis-Derived Outer Membrane Vesicles Enhance Antitumor Immunity Against Colon Tumors by Modulating CXCL10 and CD8+ T Cells

Liang,

Li,

Yang

et al. 2024

DDDT

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Given the potent immunostimulatory effects of bacterial outer membrane vesicles (OMVs) and the significant anti-colon tumor properties of Parabacteroides distasonis (Pd), this study aimed to elucidate the role and potential mechanisms of Pd-derived OMVs (Pd-OMVs) against colon cancer. Methods: This study isolated and purified Pd-OMVs from Pd cultures and assessed their characteristics. The effects of Pd-OMVs on CT26 cell uptake, proliferation, and invasion were investigated in vitro. In vivo, a CT26 colon tumor model was used to investigate the anti-colon tumor effects and underlying mechanisms of Pd-OMVs. Finally, we evaluated the biosafety of Pd-OMVs. Results: Purified Pd-OMVs had a uniform cup-shaped structure with an average size of 165.5 nm and a zeta potential of approximately −9.56 mV, and their proteins were associated with pathways related to immunity and apoptosis. In vitro experiments demonstrated that CT26 cells internalized the Pd-OMVs, resulting in a significant decrease in their proliferation and invasion abilities. Further in vivo studies confirmed the accumulation of Pd-OMVs in tumor tissues, which significantly inhibited the growth of colon tumors. Mechanistically, Pd-OMVs increased the expression of CXCL10, promoting infiltration of CD8 + T cells into tumor tissues and expression of pro-inflammatory factors TNF-α, IL-1β, and IL-6. Notably, Pd-OMVs demonstrated a high level of biosafety. Conclusion: This paper elucidates that Pd-OMVs can exert significant anti-colon tumor effects by upregulating the expression of the chemokine CXCL10, thereby increasing the infiltration of CD8 + T cells into tumors and enhancing antitumor immune responses. This suggests that Pd-OMVs may be developed as a novel nanoscale potent immunostimulant with great potential for application in tumor immunotherapy. As well as developed as a novel nano-delivery carrier for combination with other antitumor drugs.

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