Isolation, Identification and Pollution Prevention of Bacteria and Fungi during the Tissue Culture of Dwarf Hygro (Hygrophila polysperma) Explants

Li, Weijie; Cao, Guojian; Zhu, Mengqian; Zhang, Yilin; Zhou, Rong; Zhao, Zhenyang; Guo, Yanliang; Yang, Wanli; Zheng, Bo; Tan, Jiabo; Sun, Yanling

doi:10.3390/microorganisms10122476

Cited by 2 publications

(1 citation statement)

References 55 publications

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“…Contamination by fungi was indicated by the presence of thread-like structures (hyphae) and white to grey fungi colonies. Translucent to white mucoid structures that appeared on the media is bacteria colonies [17]. Contaminations could happen due to the inability of sterilant to eliminated the contaminants from the surface of the explant.…”

Section: Resultsmentioning

confidence: 99%

In vitro culture of wild Vanda tricolor Lindley seeds from Kintamani, Bali, as initial step for conservation

Gayatri,

Astarini,

Pharmawati

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Vanda tricolor is an endemic orchid from Indonesia that can be found in Bali. As part of Orchidaceae, Vanda tricolor is facing the same problem as other orchid species, their seeds have various requirements for germination which causes a low germination rate. Preventing this endangered orchid from extinction with tissue culture not only maintains the genetic diversity but also understanding the growth factors of this very species. The aim of this research was to analyze how sterilization, medium, and light affect the germination of V. tricolor. Wild V. tricolor pod from Buahan Village, Kintamani, Bangli Regency, Bali, was sterilized with 2g/L fungicide, 1,05% and 0,79% NaClO; while seeds were sterilized with 1,05% NaClO. The seeds from these two different sterilization methods were cultured on Murashige and Skoog (MS) medium, Vacin and Went (VW) medium, Organic 1 (O1) medium, and Organic 2 (O2) medium, then half of them were placed under LED light and the rest were kept on dark. Each one of the sixteen treatment combinations was replicated six times. Seed sterilization with 1,05% NaClO showed better results than pod sterilization. Seeds started to germinate 3 weeks after sown with the highest germination rate occurred on seeds cultured on MS medium under LED light. This study revealed tissue culture with different treatments will aid to understand the preferred germination condition for wild Vanda tricolor and support its conservation.

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Section: Resultsmentioning

confidence: 99%

In vitro culture of wild Vanda tricolor Lindley seeds from Kintamani, Bali, as initial step for conservation

Gayatri,

Astarini,

Pharmawati

2023

IOP Conf. Ser.: Earth Environ. Sci.

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In vitro propagation of triploid Toxicodendron vernicifluum (Stokes) F. A. Barkl

Zhang,

Feng,

Luo

et al. 2024

In Vitro Cell.Dev.Biol.-Plant

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Triploid lacquer trees have a higher yield of raw lacquer and greater stress resistance; however, it is challenging to develop efficient and rapid seedling culture techniques because of the influence of metabolites with raw lacquer. In this study, the stem segments of a triploid lacquer tree were used to investigate the effects of explant sterilization treatment, different combinations of exogenous hormone concentrations on the induction of axillary buds, seedling development, and rooting, as well as transplanting substrate ratios. The results showed that the optimal combination of disinfection treatment for triploid Toxicodendron vernicifluum (Stokes) F. A. Barkl explants involves 75% alcohol immersion for 10 s, followed by 2.0% benzalkonium chloride treatment for 1 min and 0.1% HgCl2 treatment for 4 min. The suitable medium for axillary bud induction was Murashige and Skoog (MS) containing 0.1 mg•L−1 zeatin (ZT) and 0.1 mg•L−1 6-benzylaminopurine (6-BA), resulting in a high induction rate of 95.0%. Use of 1/2 MS supplemented with 0.5 mg•L−1 6-BA and 0.3 mg•L−1 2,4-dichlorophenoxyacetic acid (2,4-D) was the most effective for axillary bud proliferation. The combination of 1/2 MS containing 0.1 mg•L−1 6-BA, 0.5 mg•L−1 2,4-D, 0.5 mg•L−1 indole-3-butyric acid (IBA), and 0.2% activated carbon (AC) was an optimum for hardening-off. With woody plant medium (WPM) supplemented with 0.5 mg•L−1 naphthaleneacetic acid (NAA) and 0.5 mg•L−1 2,4-D and 0.5 g•L−1 AC as a rooting medium, the rooting rate was 67.8%. Also, the appropriate transplanting substrate ratio for triploid Toxicodendron vernicifluum (Stokes) F. A. Barkl was grass charcoal:laterite:humus = 1:1:1. Collectively, the results provided the technical assurance for seedling scale multiplication and propagation, facilitating its application in production.

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Isolation, Identification and Pollution Prevention of Bacteria and Fungi during the Tissue Culture of Dwarf Hygro (Hygrophila polysperma) Explants

Cited by 2 publications

References 55 publications

In vitro culture of wild Vanda tricolor Lindley seeds from Kintamani, Bali, as initial step for conservation

In vitro culture of wild Vanda tricolor Lindley seeds from Kintamani, Bali, as initial step for conservation

In vitro propagation of triploid Toxicodendron vernicifluum (Stokes) F. A. Barkl

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